WDR5 (NM_017588) Human Untagged Clone

CAT#: SC110220

WDR5 (untagged)-Human WD repeat domain 5 (WDR5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR5
• Synonyms: BIG-3; CFAP89; SWD3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110220 sequence for NM_017588 edited (data generated by NextGen Sequencing)
  ATGGCGACGGAGGAGAAGAAGCCTGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
  TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
  CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
  GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
  AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
  CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
  TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
  TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
  GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
  CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
  GCCTCAGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
  AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
  TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
  TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
  AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
  ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
  CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAA
  
  Clone variation with respect to NM_017588.2
  24 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017588 unedited
  NGGTTCACATTTGTAAACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCTGAGTC
  CGCGCTCCCGCCCAGCGGCGGCCGACGCGACGCCCCGAGCGCCCGGCCCCGCCGCCGCGG
  CCCGGCAGACTGCCTCTGTCACCGGGTCCCTCCACCCTTGTCTCCTGTGCGGCCAGCGTC
  AGAGCCATGGCGACGGAGGAGAAGAAGCCTGAGACCGAGGCCGCCAGAGCACAGCCAACC
  CCTTCGTCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAG
  TTCACCCTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAG
  TGGCTGGCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAA
  TTTGAGAAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGAT
  TCTAACCTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCG
  GGCAAGTGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAAT
  CCCCAGTCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTG
  AAAACAGGGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCA
  TTTTAATCGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTG
  GGACACCGCCTCAGGCCAGTGCCCTGAGACGCTCATCGATGACGACAACCCCCCCGTGTC
  TTTTGTGAAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGGACACACTCT
  GAAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017588 unedited
  CAGTTTTAATCTTTTTTTTTTAAAAAACTGAAATTTTTTATTTTCAAAAAGAAGTATTTT
  TACATAAATCTACAACAGAATTCAAATGTAGAAAAAATAAACTATATTCAGGGATACAGC
  GCTCAGCACAGCACACCCCCTCCCCGGAGTGGGCCGTCCCCCCAGAACAAGACTGAAGGG
  GCCTCGCCGGCCCCAGGACCCCCACAGCAGGGCAGCCACAGGCTGGCACGCGGAGCTCCC
  CGTGTCTGTCACAACAGACTGCAAATGCGAGGCGCCCAGACCCTGAGCGTCGAAGACAAA
  AACCAACCCATTTTCACTGTGGCAACTCACTTCTTAGTGCAGGTCACTCATCCGTACACC
  TCATCGGAAACAGAGGGGCAGGGGAAACCACAGGATATACATCTCCAGATGCCTCAGATA
  ATCCCCTCCTCACCCCTGCCACTCGAGGACCTCCAGGAGAGCCCACCTGACAAACCCCCC
  GCTTCCTCCCTGGGCCGGTGCAACCAAAAACCTGCAGGATTCATCCAGGACTCCAGGCCC
  TGAGAAAGGCAGAGCTGGCTGAGCTCCCCCAGGACTCCAGGCCCTGAGAGAGGCAGAGCT
  GGCTGGGCTCCGGNGCCAGGGCCCTCTGTAGACCCAGGACCCAAGCTGGCTTCCCAGAGC
  ATCACAGCCCCAAAGCCCTGCCCCGTGGGACAGACCCAGGCACCTGTGCCCTCAGCCACC
  CTGCTCCGCCCTCACTGTGTGTGGGCACCCTCNACACACTGTCTNCATGGGCAGGTTCAG
  GTTNCAGCCTCGCTCCCCAGAGGCTCCCGCTCGGCCCCTGCTGGTAACTGCTGTTGCAGT
  GAGCACCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017588
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017588.2, NP_060058.1
• RefSeq Size: 3163 bp
• RefSeq ORF: 1005 bp
• Locus ID: 11091
• UniProt ID: P61964
• Cytogenetics: 9q34.2
• Domains: WD40
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. The two variants encode the same protein.