HE4 (WFDC2) (NM_006103) Human Untagged Clone

SKU SC110148

WFDC2 (untagged)-Human WAP four-disulfide core domain 2 (WFDC2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HE4
• Synonyms: dJ461P17.6; EDDM4; HE4; WAP5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006103 edited; ATGCCTGCTTGTCGCCTAGGCCCGCTAGCCGCCGCCCTCCTCCTCAGCCTGCTGCTGTTC; GGCTTCACCCTAGTCTCAGGCACAGGAGCAGAGAAGACTGGCGTGTGCCCCGAGCTCCAG; GCTGACCAGAACTGCACGCAAGAGTGCGTCTCGGACAGCGAATGCGCCGACAACCTCAAG; TGCTGCAGCGCGGGCTGTGCCACCTTCTGCTCTCTGCCCAATGATAAGGAGGGTTCCTGC; CCCCAGGTGAACATTAACTTTCCCCAGCTCGGCCTCTGTCGGGACCAGTGCCAGGTGGAC; AGCCAGTGTCCTGGCCAGATGAAATGCTGCCGCAATGGCTGTGGGAAGGTGTCCTGTGTC; ACTCCCAATTTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006103 unedited; NGGGTTTTTCAAATTTTGNTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGCAC; ACCTGCACCCCGCCCGGGCATAGCACCATGCCTGCTTGTCGCCTAGGCCCGCTAGCCGCC; GCCCTCCTCCTCAGCCTGCTGCTGTTCGGCTTCACCCTAGTCTCAGGCACAGGAGCAGAG; AAGACTGGCGTGTGCCCCGAGCTCCAGGCTGACCAGAACTGCACGCAAGAGTGCGTCTCG; GACAGCGAATGCGCCGACAACCTCAAGTGCTGCAGCGCGGGCTGTGCCACCTTCTGCTCT; CTGCCCAATGATAAGGAGGGTTCCTGCCCCCAGGTGAACATTAACTTTCCCCAGCTCGGC; CTCTGTCGGGACCAGTGCCAGGTGGACAGCCAGTGTCCTGGCCAGATGAAATGCTGCCGC; AATGGCTGTGGGAAGGTGTCCTGTGTCACTCCCAATTTCTGAGCTCCAGCCACCACCAGG; CTGAGCAGTGAGGAGAGAAAGTTTCTGCCTGGCCCTGCATCTGGTTCCAGCCCACCTGCC; CTCCCCTTTTTCGGGACTCTGTATTCCCTCTTGGGCTGACCACAGCTTCTCCCTTTCCCA; ACCAATAAAGTAACCACTTTCAGCCCCCAAAACACAAACATACACCACCTTCTCACCCTT; CCCACACCACCCATTCTCCCACTATTCCCACTCCCCTTTCAATCCTAACACCACAAACAA; AAAAACCCCCTCCTTTAATTCGCGGCCGGGCTTAACGTTTTTCCCGACACAACCCCGGGG; GGGTTCCTTGGGCCCCTCCCCACGGGCCTTTCGGGCCCTGGGAAGTCCCCCTCCAGGCCC; CCCCCTTTGGCTAAAAAAAAAAGGGCCTTTTGGCGGCAAGGGGCCCTTTAAAATTTCGGG; GGGGGGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006103 unedited; NCCCTGGTGGTTACTTCTTTGGCTGGGNAAAGGGANAAGCTGTGGTCAGCCCAANAGGGA; TACAGAGTCCCGAAAAAGGGAGGGCAGGTGGGCTGGAACCAGATGCAGGGCCAGGCAGAA; ACTTTCTCTCCTCACTGCTCAGCCTGGTGGTGGCTGGAGCTCAGAAATTGGGAGTGACAC; AGGACACCTTCCCACAGCCATTGCGGCAGCATTTCATCTGGCCAGGACACTGGCTGTCCA; CCTGGCACTGGTCCCGACAGAGGCCGAGCTGGGGAAAGTTAATGTTCACCTGGGGGCAGG; AACCCTCCTTATCATTGGGCAGAGAGCAGAACGTGGCACATCCCGCGCTGCACCACTTGA; CGTTGTCAGCGCCTTCACTGTCCCAGACGCCCTCTTGCGTGCAGTTCTGGTCACCCTGGA; TCCCAGGGCTCACGCCATCCTTTTCTGCTCGTGACGCTGTTACTCTGGTATTTTTATATA; ATTTGTAGTTTAGTATGACGCCGCTGTCCTCCCCCCCCTACCCCCCCCCCCTCTTCGTCT; TTCCCCCCCGCCAGTCCCTTACTTCTCTACACCTCATAGTATTTTTATTCTCCCACTGTT; ATCCTCTTTCCTTTTTCTTCTCGCGTCGTTATGGGTTTGTGCCTCACCCCCGCCCCCCCC; CACTCCCTCCCACCCCGGACGTGGTGTTTCTTATTTCGTTGTCCCCTCCGGACCCTAATA; GTTTTGCCCCCTTCTTCCAGCCGGTTCGGCGTGGGCCCGCGTGCGTATGTGATAATCCCG; TCTTGGCCCCGCCGCCAGTTCTGGCCTGCGCTATTTGCCGTCGGCCGTGCGGCCCGTACC; TCTCCCCTTCGCCACCCGTGTCGGGCTCGTGTCTGCGCGGGCACCATCTGTGCGTAACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_006103
• Insert Size: 660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006103.3, NP_006094.3
• RefSeq Size: 570 bp
• RefSeq ORF: 375 bp
• Locus ID: 10406
• UniProt ID: Q14508
• Cytogenetics: 20q13.12
• Domains: WAP
• Protein Families: Secreted Protein, Transmembrane
• Summary: This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]