HMGN3 (NM_138730) Human Untagged Clone

CAT#: SC110044

HMGN3 (untagged)-Human high mobility group nucleosomal binding domain 3 (HMGN3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGN3
• Synonyms: PNAS-24; PNAS-25; TRIP7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110044 sequence for NM_138730 edited (data generated by NextGen Sequencing)
  ATGCCGAAGAGAAAGTCTCCAGAGAATACAGAGGGCAAAGATGGATCCAAAGTAACTAAA
  CAGGAGCCCACAAGACGGTCTGCCAGATTGTCAGCGAAACCTGCTCCACCAAAACCTGAA
  CCCAAACCAAGAAAAACATCTGCTAAGAAAGAACCTGGAGCAAAGATTAGCAGAGGTGCT
  AAAGGGAAGAAGGAGGAAAAGCAGGAAGCTGGAAAGGAAGGCACAGAAAACTGA
  
  Clone variation with respect to NM_138730.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138730 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTTCCCTGCGCGTG
  AGCAGCTGCAGCGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAGTCCGTTGCT
  TTACTTTTTGCTTCACCGACATAGTCATTATGCCGAAGAGAAAGTCTCCAGAGAATACAG
  AGGGCAAAGATGGATCCAAAGTAACTAAACAGGAGCCCACAAGACGGTCTGCCAGATTGT
  CAGCGAAACCTGCTCCACCAAAACCTGAACCCAAACCAAGAAAAACATCTGCTAAGAAAG
  AACCTGGAGCAAAGATTAGCAGAGGTGCTAAAGGGAAGAAGGAGGAAAAGCAGGAAGCTG
  GAAAGGAAGGCACAGAAAACTGAATCTGTAGATAACGAGGGAGAATGAATTGTCATGAAA
  AATTGGGGTTGATTTTATGTATCTCTTGGGACAACTTTTAAAAGCTATTTTTACCAAGTA
  TTTTGTAAATGCTAATTTTTTAGGACTCTACTAGTTGGCATACGAAAATATATAAGGATG
  GACATTTTATCGTCTCATAGTCATGCTTTTTGGAAATTTACATCATCCTCAAGTAAAATA
  AATATCAGTTAAATATTGGAANGCTGTGTGTAAGATTGATTCAGCATTCCATGCACTTGC
  TTTAAAATTTAGTCCTGTGCATACTGGNNGGTGTTTNTACTGTGCATATTTGAAATTTTC
  ATGCAGTTTTTCTAGAGCATATCAGTGGTGCTTTTGACCCTAGTTTTTATGTGATTTTAT
  GAACTGGATATNGTTGGGGCCACTGCTGACATTTTTGTGGTTAAATAAAAGGTTTTACTT
  GTCTGCAAAAAAAAAAAAACTCATTCTGATTGGGGCGCGGTCATAACTGTTCTGAAAATA
  ACCGGTGGACATCCTGGGACCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138730 unedited
  TTTAAGGTCTACTATGNACCGCGCCGCAATCTANGATCGATTTTTTTTTTTTTTTTTTGC
  AGACAAGTAAACCTTTTATTTTAAACCACAAATAGTCAGCAGGTGGCCACAACTATCCAT
  GTTTCATTAAAATCACATAAAACCTAGGTACAAAAGCACCACTGATTATTGCTCTAGAAA
  AACTGCATGAAAAATTCAAATATGCACAGTAAAAACACCACAGTATGCACAGGACTAAAT
  TTTAAAGCAAGTGCATGGAATGCTGAATCAATCTTACACACAGCTTCCAATATTTAACTG
  ATATTTATTTTACTTGAGGATGATGTAAATTTCCAAAAAGCATGACTATGAGACGATAAA
  ATGTCCATCCTTATATATTTTCGTATGCCAACTAGTAGAGTCCTAAAAAATTAGCATTTA
  CAAAATACTTGGTAAAAATAGCTTTTAAAAGTTGTCCCAAGAGATACATAAAATCAACCC
  CAATTTTTCATGACAATTCATTCTCCCTCGTTATCTACAGATTCAGTTTTCTGTGCCTTC
  CTTTCCAGCTTCCTGCTTTTCCTCCTTCTTCCCTTTAGCACCTCTGCTAATCTTTGCTCC
  AGGTTCTTTCTTAGCAGATGTTTTTCTTGGTTTGGGTTCAAGTTTTGGTGGAGCAAGTTT
  CGCTGACAATCTGGCAGACCGTCTTGTGGGCTCCTGTTTAGTTACTTTGGATCCATCTTT
  GCCCTCTGTATTCTCTGGAGACTTTCTCTTCGGCATAATGACTATGTCGGTGAAGCAAAA
  AGTAAAGCAACGGACTGGAACTGCTGGCGCCGCCGCTGGATGCTGCCTCTGCCGCTGCAN
  CTGCTCACGCGCAGGGAACGACCCTCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138730
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138730.1, NP_620058.1
• RefSeq Size: 899 bp
• RefSeq ORF: 234 bp
• Locus ID: 9324
• UniProt ID: Q15651
• Cytogenetics: 6q14.1
• Domains: HMG14_17
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene binds thyroid hormone receptor beta in the presence of thyroid hormone. The encoded protein, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is a related pseudogene on chromosome 1. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.