SMRC2 (SMARCC2) (NM_139067) Human Untagged Clone

SKU SC109778

SMARCC2 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SMRC2
• Synonyms: BAF170; CRACC2; CSS8; Rsc8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_139067 edited; GAATTCGGCACGAGGAGATGGCGGTGCGGAAGAAGGACGGCGGCCCCAACGTGAAGTACT; ACGAGGCCGCGGACACCGTGACCCAGTTCGACAACGTGCGGCTGTGGCTCGGCAAGAACT; ACAAGAAGTATATACAAGCTGAACCACCCACCAACAAGTCCCTGTCTAGCCTGGTTGTAC; AGTTGCTACAATTTCAGGAAGAAGTTTTTGGCAAACATGTCAGCAATGCACCGCTCACTA; AACTGCCGATCAAATGTTTCCTAGATTTCAAAGCGGGAGGCTCCTTGTGCCACATTCTTG; CAGCTGCCTACAAATTCAAGAGTGACCAGGGATGGCGGCGTTACGATTTCCAGAATCCAT; CACGCATGGACCGCAATGTGGAAATGTTTATGACCATTGAGAAGTCCTTGGTGCAGAATA; ATTGCCTGTCTCGACCTAACATTTTTCTGTGCCCAGAAATTGAGCCCAAACTACTAGGGA; AATTAAAGGACATTATCAAGAGACACCAGGGAACAGTCACTGAGGATAAGAACAATGCCT; CCCATGTTGTGTATCCTGTCCCGGGGAATCTAGAAGAAGAGGAATGGGTACGACCAGTCA; TGAAGAGGGATAAGCAGGTTCTTCTGCACTGGGGCTACTATCCTGACAGTTACGACACGT; GGATCCCAGCGAGTGAAATTGAGGCATCTGTGGAAGATGCTCCAACTCCTGAGAAACCTA; GGAAGGTTCATGCAAAGTGGATCCTGGACACCGACACCTTCAATGAATGGATGAATGAGG; AAGACTATGAAGTAAATGATGACAAAAACCCTGTCTCCCGCCGAAAGAAGATTTCAGCCA; AGACACTGACAGATGAGGTGAACAGCCCAGATTCAGATCGACGGGACAAGAAGGGGGGAA; ACTATAAGAAGAGGAAGCGCTCCCCCTCTCCTTCACCAACCCCAGAAGCAAAGAAGAAAA; ATGCTAAGAAAGGTCCCTCAACACCTTACACTAAGTCAAAGCGTGGCCACAGAGAAGAGG; AGCAAGAAGACCTGACAAAGGACATGGACGAGCCCTCACCAGTCCCCAATGTAGAAGAGG; TGACACTTCCCAAAACAGTCAACACAAAGAAAGACTCAGAGTCGGCCCCAGTCAAAGGCG; GCACCATGACCGACCTGGATGAACAGGAAGATGAAAGCATGGAGACGACGGGCAAGGATG; AGGATGAGAACAGTACGGGGAACAAGGGAGAGCAGACCAAGAATCCAGACCTGCATGAGG; ACAATGTGACTGAACAGACCCACCACATCATCATTCCCAGCTACGCTGCCTGGTTTGACT; ACAATAGTGTTCATGCCATTGAGCGGAGGGCTCTCCCCGAGTTCTTCAACGGCAAGAACA; AGTCCAAGACTCCAGAGATCTACCTGGCCTATCGAAACTTTATGATTGACACTTACCGAC; TGAACCCCCAAGAGTATCTTACCTCTACCGCCTGCCGCCGAAACCTAGCGGGTGATGTCT; GTGCCATCATGAGGGTCCATGCCTTCCTAGAACAGTGGGGTCTTATTAACTACCAGGTGG; ATGCTGAGAGTCGACCAACCCCAATGGGGCCTCCGCCTACCTCTCACTTCCATGTCTTGG; CTGACACACCATCAGGGCTGGTGCCTCTGCAGCCCAAGACACCTCAGGGCCGCCAGGTTG; ATGCTGATACCAAGGCTGGGCGAAAGGGCAAAGAGCTGGATGACCTGGTGCCAGAGACGG; CTAAGGGCAAGCCAGAGCTGCAGACCTCTGCTTCCCAACAAATGCTCAACTTTCCTGACA; AAGGCAAAGAGAAACCAACAGACATGCAAAACTTTGGGCTGCGCACAGACATGTACACAA; AAAAGAATGTTCCCTCCAAGAGCAAGGCTGCAGCCAGTGCCACTCGTGAGTGGACAGAAC; AGGAAACCCTGCTTCTCCTGGAGGCACTGGAAATGTACAAAGATGACTGGAACAAAGTGT; CCGAGCATGTGGGAAGCCGCACACAGGACGAGTGCATCTTGCATTTTCTTCGTCTTCCCA; TTGAAGACCCATACCTGGAGGACTCAGAGGCCTCCCTAGGCCCCCTGGCCTACCAACCCA; TCCCCTTCAGTCAGTCGGGCAACCCTGTTATGAGCACTGTTGCCTTCCTGGCCTCTGTCG; TCGATCCCCGAGTCGCCTCTGCTGCTGCAAAGTCAGCCCTAGAGGAGTTCTCCAAAATGA; AGGAAGAGGTACCCACGGCCTTGGTGGAGGCCCATGTTCGAAAAGTGGAAGAAGCAGCCA; AAGTAACAGGCAAGGCGGACCCTGCCTTCGGTCTGGAAAGCAGTGGCATTGCAGGAACCA; CCTCTGATGAGCCTGAGCGGATTGAGGAGAGCGGGAATGACGAGGCTCGGGTGGAAGGCC; AGGCCACAGATGAGAAGAAGGAGCCCAAGGAACCCCGAGAAGGAGGGGGTGCTATAGAGG; AGGAAGCAAAAGAGAAAACCAGCGAGGCTCCCAAGAAGGATGAGGAGAAAGGGAAAGAAG; GCGACAGTGAGAAGGAGTCCGAGAAGAGTGATGGAGACCCAATAGTCGATCCTGAGAAGG; AGAAGGAGCCAAAGGAAGGGCAGGAGGAAGTGCTGAAGGAAGTGGTGGAGTCTGAGGGGG; AAAGGAAGACAAAGGTGGAGCGGGACATTGGCGAGGGCAACCTCTCCACCGCTGCTGCCG; CCGCCCTGGCCGCCGCCGCAGTGAAAGCTAAGCACTTGGCTGCTGTTGAGGAAAGGAAGA; TCAAATCTTTGGTGGCCCTGCTGGTGGAGACCCAGATGAAAAAGTTGGAGATCAAACTTC; GGCACTTTGAGGAGCTGGAGACTATCATGGACCGGGAGCGAGAAGCACTGGAGTATCAGA; GGCAGCAGCTCCTGGCCGACAGACAAGCCTTCCACATGGAGCAGCTGAAGTATGCGGAGA; TGAGGGCTCGGCAGCAGCACTTCCAACAGATGCACCAACAGCAGCAGCAGCCACCACCAG; CCCTGCCCCCAGGCTCCCAGCCTATCCCCCCAACAGGGGCTGCTGGGCCACCCGCAGTCC; ATGGCTTGGCTGTGGCTCCAGCCTCTGTAGTCCCTGCTCCTGCTGGCAGTGGGGCCCCTC; CAGGAAGTTTGGGCCCTTCTGAACAGATTGGGCAGGCAGGGTCAACTGCAGGGCCACAGC; AGCAGCAACCAGCTGGAGCCCCCCAGCCTGGGGCAGTCCCACCAGGGGTTCCCCCCCCTG; GACCCCATGGCCCCTCACCGTTCCCCAACCAACAAACTCCTCCCTCAATGATGCCAGGGG; CAGTGCCAGGCAGCGGGCACCCAGGCGTGGCGGACCCAGGCACCCCCCTGCCTCCAGACC; CCACAGCCCCGAGCCCAGGCACGGTCACCCCTGTGCCACCTCCACAGTGAGGAGCCAGCC; AGACATCTCTCCCCCTCACCCCCTGTGGACATCACGGTTCCAGGAACAGCCCTTCCCCCA; CCACTGGGACCCTCCCCAGCCTGGAGAGTTCATCACTACGTAAGGAAAGCTCCTTCCGCC; CCTCCAAAGCCCTCACCATGCCTAACAGAGGCATGCATTTTTATATCAGATTATTCAAGG; ACTTCTGTTTAAAAGATGTTTATAATGTCTGGGAGAGAGGATAGGATGGGAATGCTGCCC; TAAAGGAAGGGCTGGTGAAAGGTGTTTATACAAGGTTCTATTAACCACTTCTAAGGGTAC; ACCTCCCTCCAAACTACTGCATTTTCTATGGATTAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139067 unedited; CGAATTCGGCACGAGGAGATGGCGGTGCGGAAGAAGGACGGCGGCCCCAACGTGAAGTAC; TACGAGGCCGCGGACACCGTGACCCAGTTCGACAACGTGCGGCTGTGGCTCGGCAAGAAC; TACAAGAAGTATATACAAGCTGAACCACCCACCAACAAGTCCCTGTCTAGCCTGGTTGTA; CAGTTGCTACAATTTCAGGAAGAAGTTTTTGGCAAACATGTCAGCAATGCACCGCTCACT; AAACTGCCGATCAAATGTTTCCTAGATTTCAAAGCGGGAGGCTCCTTGTGCCACATTCTT; GCAGCTGCCTACAAATTCAAGAGTGACCAGGGATGGCGGCGTTACGATTTCCAGAATCCA; TCACGCATGGACCGCAATGTGGAAATGTTTATGACCATTGAGAAGTCCTTGGTGCAGAAT; AATTGCCTGTCTCGACCTAACATTTTTCTGTGCCCAGAAATTGAGCCCAAACTACTAGGG; AAATTAAAGGACATTATCAAGAGACACCAGGGAACAGTCACTGAGGATAAGAACAATGCC; TCCCATGTTGTGTATCCTGTCCCGGGGAATCTAGAAGAAGAGGAATGGGTACGACCAGTC; ATGAAGAGGGATAAGCAGGTTCTTCTGCACTGGGGCTACTATCCTGACAGTTACGACACG; TGGATCCCAGCGAGTGAAATTGAGGCATCTGTGGAAGATGCTCNCACTCCTGAGAAACTA; NGAGGTTCATGCANAGTGGATCCTGGACACCGACACCTTCATGAATGGATGANTGAGGAA; GACTATGAAGTAAATGATGACAAAAACCCTGGGTCTCCGGCCGAAGAAGATTTCAGGCCA; GAAACTGACAGATGAGGTGAACAGCCCAGATTCAAATCGACGGGACAGGAGGGGGGGAAA; CTATAGAAGAGGAGCGCTCCCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139067 unedited; TGCTATGCACCGGCGGCCGCAATCTANGGTCGAGTTTTTTTTTTTTTTTTTTAATCCATA; GAAAATGCAGTAGTTTGGAGGGAGGTGTACCCTTAGAAGTGGTTAATAGAACCTTGTATA; AACACCTTTCACCAGCCCTTCCTTTAGGGCAGCATTCCCATCCTATCCTCTCTCCCAGAC; ATTATAAACATCTTTTAAACAGAAGTCCTTGAATAATCTGATATAAAAATGCATGCCTCT; GTTAGGCATGGTGAGGGCTTTGGAGGGGCGGAAGGAGCTTTCCTTACGTAGTGATGAACT; CTCCAGGCTGGGGAGGGCCCCAGTGGTGGGGGAAGGGCTGCTCCTGGAACCGTGATGTCC; ACAGGGGGCGAGGGGGAGAGATGCCTGGCTGCCTCCTCACTGTGGAGGTGGCACAGGGGT; GACCGTGCCTGGGCTCGGCGCTGCGGGGCCTGGAGGCAGGGGGGCGCCTGGGTCCGCCAC; GCCTGCGGGCCCGCTGCCTGTCCCTCGCCTCGGCCCATCGAGGGACGAGCCCGTCGTCCT; GCCCCCGGCTGCGCGTCCACTTCCATCCCCGCCTCTCCCCCCCTTCCGTCCACCCCCCCC; CCCCGTACGTCCCCTCCGCCGAATAACAATATTATCTCATCTTTTATCCCCCCCCGCCTC; ATTTCGTACCCACACTCCACCTCCCCCGCATCCCCCCCGCCCCCCCACCCTCCCCCACTA; TCTCCCCCCCCCTCCCTCCCCTCCCCATCTCCCCTCATTTTCCCCCCTCCCCCATTTTCA; TCACCTCCCCTTTCCGCCCCCCTCCCCATTTCTACGTCCAACGCGCCTCTCCTATTCTTC; TCCATTCCCACCCCCCCCCCCCCCCATTCCCTTCCCCCTTTCCGCTCCCTCCCCCCCCTC; TGTCCCCTCCTTCGTATATTCCCTCCCTCCTCACTTTCCCCCTATCCGCCCTCCCTTTTT; CT;
• Restriction Sites: NotI-NotI
• ACCN: NM_139067
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139067.1, NP_620706.1
• RefSeq Size: 3786 bp
• RefSeq ORF: 3393 bp
• Locus ID: 6601
• UniProt ID: Q8TAQ2
• Cytogenetics: 12q13.2
• Domains: CHROMO, myb_DNA-binding, SWIRM
• Protein Families: Transcription Factors
• Summary: The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) contains an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b), contains a novel internal segment, lacks a segment near the C-terminus, and is shorter than isoform a.