NIPP1 (PPP1R8) (NM_138558) Human Untagged Clone

CAT#: SC109604

PPP1R8 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NIPP1
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_138558 edited
  AGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCAAGATGGCG
  GCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACTGCCCAACCTGGGCAGGTAAG
  CCCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATT
  ATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATT
  GACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTGAAGAGA
  GTTTTCCTGATAGATCTCAACAGTACACACGGCACTTTCTTGGGTCACATTCGGTTGGAA
  CCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATTTGGCGCATCCACAAGG
  GCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGATGAGAAG
  ATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAAACTGAG
  CTTGATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTTACCATT
  GAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAGAGGAAGAACTCACGGGTGACA
  TTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTGGATCCCTCAGTTGGTCGATTC
  AGGAACATGGTGCAAACTGCAGTGGTCCCAGTCAAGAAGAAGCGTGTGGAGGGCCCTGGC
  TCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGCGGAGGA
  CTCTACGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGCATCCAT
  GGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGATGTGGAC
  TTGACTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTAT
  AACCCTGAAGCTGTAAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGC
  AAGAAGCCCACACCTTCCTTGCTGATTTGATATTTTTGGTCATGGAGAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138558 unedited
  TTTCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCA
  GAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAA
  TTCGGCACCAGCAAGATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGAC
  TGCCCAACCTGGGCAGGTAAGCCCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGAC
  AAACTAATTGAGAAACTGATTATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCT
  GATTTGTGTGACTTTACCATTGACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTC
  TACCACAAGCATCTGAAGAGAGTTTTCCTGATAGATCTCAACAGTACACACGGCACTTTC
  TTGGGTCACATTCGGTTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTC
  TCATTTGGCGCATCCACAAGGGCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCG
  GCTGTGAAAGGAGATGAGAAGATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGG
  CTTCCAGAGGAGGAAACTGAGCTTGATAACCTGACAGAGTTCAACACTGCCCACAACAAG
  CGGATTTCTACCCTTACCATTGAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAG
  AGGAAGAACTCACGGGTGACATTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTG
  GATCCCTCAGTTGGTCGATTCANGAACATGGTGCANACTGCAGTGGTCCCAGTCAAGAAA
  AAGCGTGTGGNAGGCCCCTGGNCTCCTGGGGCCTGGNAGAATCAGGNAGCANGCGCATGG
  CAGACTTTGCCTTCAGCGGAGGACTCTACGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138558 unedited
  TCTTGAACCGCGCCGCTTTCTAGGATCGAGTTTTTCTTTTTTTTTTTTTTTTTTTTTTTG
  ACAAAAGTTACGTTTAATTTACAATGTAATAAAGGTTACAGGTAAAAAGCTACACATAAA
  GCATGAAAAGGCCTATTACACAAATGTACAAATCTCTGTACAAAGCTTGTATCAATGTTT
  CCTAACTTTGTCTCCTATGTTTGTTAGCCAGGTCTAGTCTTGGAGCTCTGAATAACAAGA
  GCTCTCAATTGAGGGGGGAAAGAGCCTCCAAACTGACATCTAAAAATTCCTCTTAGAGAC
  ACCAGTTACTTCCAAACAAATCTTTTCCTTTTTAATTTGAATTACCCCAACTGGTCATAT
  CCTTTGAAAACTGCCTTCATAATACACTTAAAAGTAATGCCTTAGAGTAGAGGACAGTAC
  TCTGGAATTAAATAAGAAACTTCAGCAGGAGGAGTGCTCAGGTTTGACAAGAAAAACAGG
  TAAATTTCAAACAATACTGTAAGTTAAAACTATAAACCTGTGTCTATGGCACCAGTCACA
  GATGAAGGAAAATCGAAATGTTTTCATTCCAACAGGAAGGACAAAAGAGAAAAGCCTAGC
  TACTGGACCTCAAAACCACTGACCCAAGGAAACATTTTTTAGTTTAAACCTCAGCTTAAC
  TGCAGAATTCTAAGCCTTCTTTTTGATCAAACTGAGATCTATGGCAACCCTTCTGCTACC
  CGGGACTTGGAAGGATAGTGAATATAAATTAGGCTCTGGACCCCCAAGGCTGGTGATGCT
  ACTGTAACCTCTACAGCTCTGGATTCAACCTGAGGGCCTGGGATATGTGACTTTCATAAA
  AATGATCCTAGAAAAGTGGAGAAAAAAGGAGGTAGTGNAAAGCCCTAGAAAGGACCCAGG
  CAGTCTATTGTAAAGGATATAGAACCCTTTCTCTACATGCAGTGGTGTGAAAACAGTCTT
  TTGGGCTATTCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138558
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138558.1, NP_612568.1
• RefSeq Size: 2691 bp
• RefSeq ORF: 630 bp
• Locus ID: 5511
• UniProt ID: Q12972
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) is the longest transcript and has its first exon longer at the 3' end as compared to that of transcript variant 1. The resulting isoform (beta/delta) uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform beta/delta contains protein phosphatase inhibitory activity.