L1CAM (NM_000425) Human Untagged Clone

SKU SC109376

L1CAM (untagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: L1CAM
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109376 sequence for NM_000425 edited (data generated by NextGen Sequencing); ATGGTCGTGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATC; CAGATCCCCGAGGAATATGAAGGACACCATGTGATGGAGCCACCTGTCATCACGGAACAG; TCTCCACGGCGCCTGGTTGTCTTCCCCACAGATGACATCAGCCTCAAGTGTGAGGCCAGT; GGCAAGCCCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCACTTCAAACCCAAGGAA; GAGCTGGGTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGGGCAAC; AACAGCAACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTG; GGCACCGCCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAG; GAGACAGTGAAGCCCGTGGAGGTGGAGGAAGGGGAGTCAGTGGTTCTGCCTTGCAACCCT; CCCCCAAGTGCAGAGCCTCTCCGGATCTACTGGATGAACAGCAAGATCTTGCACATCAAG; CAGGACGAGCGGGTGACGATGGGCCAGAACGGCAACCTCTACTTTGCCAATGTGCTCACC; TCCGACAACCACTCAGACTACATCTGCCACGCCCACTTCCCAGGCACCAGGACCATCATT; CAGAAGGAACCCATTGACCTCCGGGTCAAGGCCACCAACAGCATGATTGACAGGAAGCCG; CGCCTGCTCTTCCCCACCAACTCCAGCAGCCACCTGGTGGCCTTGCAGGGGCAGCCATTG; GTCCTGGAGTGCATCGCCGAGGGCTTTCCCACGCCCACCATCAAATGGCTGCGCCCCAGT; GGCCCCATGCCAGCCGACCGTGTCACCTACCAGAACCACAACAAGACCCTGCAGCTGCTG; AAAGTGGGCGAGGAGGATGATGGCGAGTACCGCTGCCTGGCCGAGAACTCACTGGGCAGT; GCCCGGCATGCGTACTATGTCACCGTGGAGGCTGCCCCGTACTGGCTGCACAAGCCCCAG; AGCCATCTATATGGGCCAGGAGAGACTGCCCGCCTGGACTGCCAAGTCCAGGGCAGGCCC; CAACCAGAGGTCACCTGGAGAATCAACGGGATCCCTGTGGAGGAGCTGGCCAAAGACCAG; AAGTACCGGATTCAGCGTGGCGCCCTGATCCTGAGCAACGTGCAGCCCAGTGACACAATG; GTGACCCAATGTGAGGCCCGCAACCGGCACGGGCTCTTGCTGGCCAATGCCTACATCTAC; GTTGTCCAGCTGCCAGCCAAGATCCTGACTGCGGACAATCAGACGTACATGGCTGTCCAG; GGCAGCACTGCCTACCTTCTGTGCAAGGCCTTCGGAGCGCCTGTGCCCAGTGTTCAGTGG; CTGGACGAGGATGGGACAACAGTGCTTCAGGACGAACGCTTCTTCCCCTATGCCAATGGG; ACCCTGGGCATTCGAGACCTCCAGGCCAATGACACCGGACGCTACTTCTGCCTGGCTGCC; AATGACCAAAACAATGTTACCATCATGGCTAACCTGAAGGTTAAAGATGCAACTCAGATC; ACTCAGGGGCCCCGCAGCACAATCGAGAAGAAAGGTTCCAGGGTGACCTTCACGTGCCAG; GCCTCCTTTGACCCCTCCTTGCAGCCCAGCATCACCTGGCGTGGGGACGGTCGAGACCTC; CAGGAGCTTGGGGACAGTGACAAGTACTTCATAGAGGATGGGCGCCTGGTCATCCACAGC; CTGGACTACAGCGACCAGGGCAACTACAGCTGCGTGGCCAGTACCGAACTGGATGTGGTG; GAGAGTAGGGCACAGCTCTTGGTGGTGGGGAGCCCTGGGCCGGTGCCACGGCTGGTGCTG; TCCGACCTGCACCTGCTGACGCAGAGCCAGGTGCGCGTGTCCTGGAGTCCTGCAGAAGAC; CACAATGCCCCCATTGAGAAATATGACATTGAATTTGAGGACAAGGAAATGGCGCCTGAA; AAATGGTACAGTCTGGGCAAGGTTCCAGGGAACCAGACCTCTACCACCCTCAAGCTGTCG; CCCTATGTCCACTACACCTTTAGGGTTACTGCCATAAACAAATATGGCCCCGGGGAGCCC; AGCCCGGTCTCTGAGACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGAT; GTGAAGGGGGAAGGAAATGAGACCACCAATATGGTCATCACGTGGAAGCCGCTCCGGTGG; ATGGACTGGAACGCCCCCCAGGTTCAGTACCGCGTGCAGTGGCGCCCTCAGGGGACACGA; GGGCCCTGGCAGGAGCAGATTGTCAGCGACCCCTTCCTGGTGGTGTCCAACACGTCCACC; TTCGTGCCCTATGAGATCAAAGTCCAGGCCGTCAACAGCCAGGGCAAGGGACCAGAGCCC; CAGGTCACTATCGGCTACTCTGGAGAGGACTACCCCCAGGCAATCCCTGAGCTGGAAGGC; ATTGAAATCCTCAACTCAAGTGCCGTGCTGGTCAAGTGGCGGCCGGTGGACCTGGCCCAG; GTCAAGGGCCACCTCCGCGGATACAATGTGACGTACTGGAGGGAGGGCAGTCAGAGGAAG; CACAGCAAGAGACATATCCACAAAGACCATGTGGTGGTGCCCGCCAACACCACCAGTGTC; ATCCTCAGTGGCTTGCGGCCCTATAGCTCCTACCACCTGGAGGTGCAGGCCTTTAACGGG; CGAGGATCGGGGCCCGCCAGCGAGTTCACCTTCAGCACCCCAGAGGGAGTGCCTGGCCAC; CCCGAGGCGTTGCACCTGGAGTGCCAGTCGAACACCAGCCTGCTGCTGCGCTGGCAGCCC; CCACTCAGCCACAACGGCGTGCTCACCGGCTACGTGCTCTCCTACCACCCCCTGGATGAG; GGGGGCAAGGGGCAACTGTCCTTCAACCTTCGGGACCCCGAACTTCGGACACACAACCTG; ACCGATCTCAGCCCCCACCTGCGGTACCGCTTCCAGCTTCAGGCCACCACCAAAGAGGGC; CCTGGTGAAGCCATCGTACGGGAAGGAGGCACTATGGCCTTGTCTGGGATCTCAGATTTT; GGCAACATCTCAGCCACAGCGGGTGAAAACTACAGTGTCGTCTCCTGGGTCCCCAAGGAG; GGCCAGTGCAACTTCAGGTTCCATATCTTGTTCAAAGCCTTGGGAGAAGAGAAGGGTGGG; GCTTCCCTTTCGCCACAGTATGTCAGCTACAACCAGAGCTCCTACACGCAGTGGGACCTG; CAGCCTGACACTGACTACGAGATCCACTTGTTTAAGGAGAGGATGTTCCGGCACCAAATG; GCTGTGAAGACCAATGGCACAGGCCGCGTGAGGCTCCCTCCTGCTGGCTTCGCCACTGAG; GGCTGGTTCATCGGCTTTGTGAGTGCCATCATCCTCCTGCTCCTCGTCCTGCTCATCCTC; TGCTTCATCAAGCGCAGCAAGGGCGGCAAATACTCAGTGAAGGATAAGGAGGACACCCAG; GTGGACTCTGAGGCCCGACCGATGAAAGATGAGACCTTCGGCGAGTACAGGTCCCTGGAG; AGTGACAACGAGGAGAAGGCCTTTGGCAGCAGCCAGCCATCGCTCAACGGGGACATCAAG; CCCCTGGGCAGTGACGACAGCCTGGCCGATTATGGGGGCAGCGTGGATGTTCAGTTCAAC; GAGGATGGTTCGTTCATTGGCCAGTACAGTGGCAAGAAGGAGAAGGAGGCGGCAGGGGGC; AATGACAGCTCAGGGGCCACTTCCCCCATCAACCCTGCCGTGGCCCTAGAATAG; ; Clone variation with respect to NM_000425.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000425 unedited; NTTTGTTCGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGG; CGGCGGGTCCGAGGCCGGCGTGCGCGGAGGCTGGGCGGGCAGCCCGAGCGGTGGCCGCAG; CGCAGTGCCCCCACTCCCAACTCCCGCCCCAAGCCGCCCACCAGCCCCCTTCCCCTCCGG; CCGGAGCCTGAACCGAGCCCGGCTGGCTGTGCTGCGCGGTGCCGCCGGGAAAGATGGTCG; TGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATCCAGATCC; CCGAGGAATATGAAGGACACCATGTGATGGAGCCACCTGTCATCACGGAACAGTCTCCAC; GGCGCCTGGTTGTCTTCCCCACAGATGACATCAGCCTCAAGTGTGAGGCCAGTGGCAAGC; CCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCACTTCAAACCCAAGGAAGAGCTGG; GTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGGGCAACAACAGCA; ACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTGGGCACCG; CCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAGGAGACAG; TGAAGCCCGTGGAGGTGGAGGAAGGGNAGTCAGTGGNTCTGCCTTGCAACCCTNCCCCCA; GTGCAGAGCCTCTCCGGATCTACTGGATGAACAGCAAGATCTTGCACATCAAGCAGGACG; AAGCGGGTGACGATGGCCCAGAACGGGCACCTCTACTTTGCCATGTGCTCACCTNCGACA; CCACTCAGATACATCTGCCACGCCCACTTCCAAGCAACCAGACATCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000425 unedited; NNTTTTACTCTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAT; TTTCAGGTTTTATTTGCCTGTTATAATTCTGATATTATGTACATATATATATATACTTTT; GTTTTTTTGTAGAGGGCTTATCAGTAGACCAAGCACAGGCATACAGGGAGGCTGTGGCTG; CTGAGCAGTGCTGGGAGAACCAATCTTTCCTTTTTTTTTGTCTGTTTTTATTTTTCCTGG; GATGGGTTCATCAAAGCTAATTGCTATCAATCCTAGAAACCACCTAGGGACTCAGACATC; TGCCTACACACTAGTGGCGTAAAGGGAAGGACAGGGGTACAAACTTTCAAAGTCACGGTG; TATTTACATTCCCAAGGTGGGGGCGGGAGGAGGCTGAGGAAGACAACTGTTCAGACGATA; GGGAGGGCAGGGCTGCTAAGGGGTCCTGGGGGGTGGGGGCGGCTGGAAGAGGCCGGCCAG; TGGGCAGTGGGAGGAGGTGTGCCCGCCCCGCAGCCAGGTAGTGACGGAGGCCACTTGGAT; GTTGTGTGGTGGGTACCGAAGGCAGCGTGTGTATGGAGCTCCTGAAAGCCGGCATGGGGT; GGGCTGGGCGGCGGGAGAAGGCGGTGCTGCCAGAGTGCGATGCTGGGGAGTGGNGGGACT; CTGGGCTCTNCAGATGGCCTCCCCTGCCCCCGCGGCCAGTTTGCCCGGCTCCCATCCCGG; TGGAGCANAGATGGCAAAGAAAAGAAGCAGATGGTGGGGACACCCGGTTCTGTTCCCAGT; CCCACCCAAGCTGCTGGGGAATGGCTTGGGGAGAGGGAAAAAGGGCCAACCTTGGTCCTG; AGGAAACCCCCCATTTTTGTCCCCACTTCCGGCACCAAGAAAGACAAATTTTCGGGAAAC; ACTTTTGGGCCACCCCTTACCTTTTCTTCCCCAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000425
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000425.2, NP_000416.1
• RefSeq Size: 4525 bp
• RefSeq ORF: 3774 bp
• Locus ID: 3897
• UniProt ID: P32004
• Cytogenetics: Xq28
• Domains: FN3, ig, IG, IGc2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)
• Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]; Transcript Variant: This variant (1, also known as FL-L1CAM) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 4. Variants 1 and 4 encode the same isoform (1).