hnRNP A1 (HNRNPA1) (NM_002136) Human Untagged Clone

CAT#: SC109308

HNRNPA1 (untagged)-Human heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hnRNP A1
• Synonyms: ALS19; ALS20; hnRNP-A1; hnRNP A1; HNRPA1; HNRPA1L3; IBMPFD3; UP 1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109308 sequence for NM_002136 edited (data generated by NextGen Sequencing)
  ATGTCTAAGTCAGAGTCTCCTAAAGAGCCCGAACAGCTGAGGAAGCTCTTCATTGGAGGG
  TTGAGCTTTGAAACAACTGATGAGAGCCTGAGGAGCCATTTTGAGCAATGGGGAACGCTC
  ACGGACTGTGTGGTAATGAGAGATCCAAACACCAAGCGCTCCAGGGGCTTTGGGTTTGTC
  ACATATGCCACTGTGGAGGAGGTGGATGCAGCTATGAATGCAAGGCCACACAAGGTGGAT
  GGAAGAGTTGTGGAACCAAAGAGAGCTGTCTCCAGAGAAGATTCTCAAAGACCAGGTGCC
  CACTTAACTGTGAAAAAGATATTTGTTGGTGGCATTAAAGAAGACACTGAAGAACATCAC
  CTAAGAGATTATTTTGAACAGTATGGAAAAATTGAAGTGATTGAAATCATGACTGACCGA
  GGCAGTGGCAAGAAAAGGGGCTTTGCCTTTGTAACCTTTGACGACCATGACTCCGTGGAT
  AAGATTGTCATTCAGAAATACCATACTGTGAATGGCCACAACTGTGAAGTTAGAAAAGCC
  CTGTCAAAGCAAGAGATGGCTAGTGCTTCATCCAGCCAAAGAGGTCGAAGTGGTTCTGGA
  AACTTTGGTGGTGGTCGTGGAGGTGGTTTCGGTGGGAATGACAACTTCGGTCGTGGAGGA
  AACTTCAGTGGTCGTGGTGGCTTTGGTGGCAGCCGTGGTGGTGGTGGATATGGTGGCAGT
  GGGGATGGCTATAATGGATTTGGTAATGATGGAAGCAATTTTGGAGGTGGTGGAAGCTAC
  AATGATTTTGGGAATTACAACAATCAGTCTTCAAATTTTGGACCCATGAAGGGAGGAAAT
  TTTGGAGGCAGAAGCTCTGGCCCCTATGGCGGTGGAGGCCAATACTTTGCAAAACCACGA
  AACCAAGGTGGCTATGGCGGTTCCAGCAGCAGCAGTAGCTATGGCAGTGGCAGAAGATTT
  TAA
  
  Clone variation with respect to NM_002136.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002136 unedited
  TATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGCATCGTTAAAGTCTCTCTTC
  ACCCTGCCGTCATGTCTAAGTCAGAGTCTCCTAAAGAGCCCGAACAGCTGAGGAAGCTCT
  TCATTGGAGGGTTGAGCTTTGAAACAACTGATGAGAGCCTGAGGAGCCATTTTGAGCAAT
  GGGGAACGCTCACGGACTGTGTGGTAATGAGAGATCCAAACACCAAGCGCTCCAGGGGCT
  TTGGGTTTGTCACATATGCCACTGTGGAGGAGGTGGATGCAGCTATGAATGCAAGGCCAC
  ACAAGGTGGATGGAAGAGTTGTGGAACCAAAGAGAGCTGTCTCCAGAGAAGATTCTCAAA
  GACCAGGTGCCCACTTAACTGTGAAAAAGATATTTGTTGGTGGCATTAAAGAAGACACTG
  AAGAACATCACCTAAGAGATTATTTTGAACAGTATGGAAAAATTGAAGTGATTGAAATCA
  TGACTGACCGAGGCAGTGGCAAGAAAAGGGGCTTTGCCTTTGTAACCTTTGACGACCATG
  ACTCCGTGGATAAGATTGTCATTCAGAAATACCATACTGTGAATGGCCACAACTGTGAAG
  TTAGAAAAGCCCTGTCAAAGCAAGAGATGGCTAGTGCTTCATCCAGCCAAAGAGGTCGAA
  GTGGTTCTGGAAACTTTGGTGGGTGGTCGTGGGAGGTGTTTTCGGTGGGAATGACAACTN
  TCNGTCGTGGAGGAAACTTNCAGTGGTCGTGGTGGCTTTTGTGGCAGCCGTGGTTGGTGG
  TGGATATGGTGGCAGTGGGGGATGGCTATAATGGATTTGGTAATGATGGAAGCAATTTGG
  AGTGGNTGGAGCTACAATGATTTGGGAAATACACATCAGTCTTAATTTTGGACCCTGAAG
  GGAGAATTTTGAGGCAGAGCTTTGCCCTTATGCGGTGGAGCCATACTTTGCAACCACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002136 unedited
  ACTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAAAAAAAT
  AAATCGTTTATTGATTACACATGATAATGGATACACAAGCTTCATTCCCATCTATAATTT
  TATCTGGTACCATTATTCAATTTAAATATATTGCATAGGATGTGCCAACAATCACTTTTA
  TAACCATTCCATGATTTTGCTTGGGTAATCCCTTTTAATGGTGAACTTCAGGTCACAACA
  GTAACTATCAGTTCAACTACACCAAGGTTTCCGAAGACAATGGCTTCTCCACCCAAGCAG
  GTTGTATATAAATTCCAAATAGAACCTGGCATCACCCTGAAGGAATTCTAACTTCACACT
  GTTGGGGAAATTTACCAAGATGGCTTAAGAGTAGACTAACTTTACACAGCACATTAAAAA
  AAAGACATTTATTCAGCGTCACGATCAGACTGTTACATTTAGCAATCAACAGCATGGGGT
  GCAAAAAAAAAAATCTACATTAAAACCCTTTGTTGGAATGCTTTACACTTTCCACAGAAC
  AGAAACTAAAATAACCTGTTATACAATTAGTCACAAATACAGTCCTCGAGTTTTTTGCCC
  ATACACATGAGTATTTGTCTAAAACATGTCTTCTTTGTAGCAGCTAGGCCCTGCCACCAC
  TGTGCTTGGCTGAGTTCACAAATCTGTTGTAACCTGAAGCTTCCCTGTCACTTCTCTGGC
  TCTCCTCTCCTGCTAAGCTTTGTTTCCTAATTAAATTTTCTGCCACTGCCATAGCTACTG
  CTGCTGCTGGAAACCGCATAGCCACCTTGTTTTCCGGGTTTTGCAAGTATTGCCCTCCAC
  CGCATAGGGCCAAAGCTTCTGCTCCAAAATCCCTCCCTCATGGGCCAAAATTTGAAACGA
  TTGGGGNAATTCCAAATATTTGAGCTCCCCACCTCCAAATGGTTCCTCTTTCCAATACCT
  TTACCCTCCCCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002136
• Insert Size: 1840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002136.1, NP_002127.1
• RefSeq Size: 1769 bp
• RefSeq ORF: 963 bp
• Locus ID: 3178
• UniProt ID: P09651
• Cytogenetics: 12q13.13
• Domains: RRM
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1, also known as A1) encodes isoform a.