PYK2 (PTK2B) (NM_004103) Human Untagged Clone

CAT#: SC109209

PTK2B (untagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYK2
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004103 edited
  ATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAA
  GGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATC
  CTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAA
  TGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATC
  GGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGAT
  GAGATCCACTGGCTGCACCCACAGATGACAGTGGGTGAGGTGCAGGACAAGTATGAGTGT
  CTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTC
  ATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAAC
  GACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGC
  CTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAAC
  TTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAG
  AACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCG
  CTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATC
  GACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC
  ATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCC
  GAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTT
  CAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAG
  GCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGC
  TCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCC
  ATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGTCAGAC
  ATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATT
  GCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTAT
  GAAGGTGTCTACACAAATCACAAAGGGGAGAAAACCAATGTAGCTGTCAAGACCTGCAAG
  AAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAAC
  CTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCTGGATC
  ATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCC
  CTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTACCTG
  GAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCT
  GAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTAC
  AAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGA
  CGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGC
  TTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAA
  GGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGC
  TGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGT
  GACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGA
  ACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCT
  AAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCT
  GAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTT
  AACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGG
  GAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAA
  AAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAG
  TGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATACGTCCCCA
  TTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCG
  AGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACCGATGACCTGGTG
  TACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAG
  CTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTC
  ATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAG
  GGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCG
  CAGCAGAACGCCGTGACCTCCCTGAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCA
  CACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTG
  GCCAATCTGGCCCACCCACCTGCAGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004103 unedited
  GGTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGAGCAGCAGG
  GGTGTGGTTAACAACTCAGAGGAGGAGGGAGAATCTAACCTGTCAGCCCTTTTACTCAGC
  CACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACTTACCTGTACTTGCCGCC
  GTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGAC
  TGCAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAG
  TCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAGAGCCCATGGTGGTGGT
  ACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAG
  CTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGA
  GATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTG
  CTATGGGCTGAGGCTGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGAT
  GACAGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTA
  TGACCTTCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGAC
  CACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGCAGCGCTACGCCAGCAA
  GGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCANGCGGTTCTTCAGGAT
  ATGCCCCACAATGCACTTGACNAGAAGTCCAACTTCGAGCTNCTAGAANAGGAAGTGGNG
  CTGGNACTGNTNTNNCCCAGCAGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004103 unedited
  ATTTGGGTTTNTAAACCACTTTCAGTCAAAAGNAAGGAGCCTAAAGNATGTTTATGCAAA
  CTCTTGANAAAANAAGGTGCAGATGAGAATGGGGGTTGGGCGAGAGAAAAGAGGAGGATG
  TAAGAAAAGCAGGGAAAAGCAAGGGAAAGTAAAGGAAGAAAGAGAAAGAGGGCAGGAAGA
  GAGCGGATTTGGCCCAAGGTCCTATCTTGGTTTTTTTTTTCTGCTTCTTCCCCCTGATGC
  TTGGTTTGTTGACAACACAGCATCCTGTGCCTGGACTCCCAATTAGCTTGTTCCTGGACT
  GTGCCCCAGGTCCTCCCTCAGGAGGGCACATGCTGTCAGTCCAGACCAAACTCACATTAA
  ATAAATTTCAATATACACTGTACAAGAATGCCAGGCCCATCCCTCATCTCACTGGCTGCC
  TGACCCCCAAAACAAAGCTCCTCCCCAGCTTCTCTGTGCATCAAGGGACATCTGAGGGCC
  GAAGAGGAAGGGAAAGGAATAGCTTGGTTCCCAAATCGGCCTGCCATGTCCATATATTAA
  AGCTTGGGGCCACCATGTGACACCTCCCCACCCCCAGGAGCCCCCTGCATGGACCCAGGC
  TGGCTTAAGGAGCAGGGGCCAGGCCGCCACAGTCCCCCTCTGAGCCATTTTTGTCACCTG
  CACCCTGGGTCCATTATCCAGAGTCCCCTTTGGCCAGCTTCCGGCTGAGCCATTCATCCC
  AGGGTATGTGTTGGGGATAGGGCGTTTTGCCCAAGTGTCTGGGGAAGGGTGACTTCCCTT
  TGGCTTTTCCCCTTGGTAGTTCCCCATGTACCCACTCCCTGCTCGGGGTGGTTCCTGGGC
  CGGCCTGGGTCTGAATACATAGTTGGGGGCCCCCATCCTTCTGTAATTATAAGGTTAATG
  GGCCCATATTGCCATAAATTTTGTCTTGGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004103
• Insert Size: 4400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004103.3, NP_004094.3
• RefSeq Size: 4555 bp
• RefSeq ORF: 3030 bp
• Locus ID: 2185
• UniProt ID: Q14289
• Cytogenetics: 8p21.2
• Domains: B41, pkinase, TyrKc, S_TKc, Focal_AT
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.