Dystrobrevin alpha (DTNA) (NM_032979) Human Untagged Clone

SKU SC109177

DTNA (untagged)-Human dystrobrevin, alpha (DTNA), transcript variant 5

Product Data

• Type: Human Untagged Clone
• Target Symbol: Dystrobrevin alpha
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032979, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATTGAAGATAGTGGGAAAAGAGGAAATACCATGGCAGAAAGAAGACAGCTGTTTGCAGAGATGAGGG; CTCAAGATCTGGATCGCATCCGACTCTCCACCTACAGAACAGCATGCAAGCTTAGGTTTGTTCAGAAGAA; ATGCAATTTGCACCTGGTGGACATATGGAATGTCATAGAAGCATTGCGGGAAAATGCTCTGAACAACCTG; GACCCAAACACTGAACTCAACGTGTCCCGCTTAGAGGCTGTGCTCTCCACTATTTTTTACCAGCTCAACA; AACGGATGCCAACCACTCACCAAATCCATGTGGAGCAGTCCATCAGCCTCCTCCTTAACTTCCTGCTTGC; AGCGTTTGATCCGGAAGGCCATGGTAAAATTTCAGTATTTGCTGTCAAAATGGCTTTAGCCACATTGTGT; GGAGGGAAGATCATGGACAAATTAAGATATATTTTCTCAATGATTTCTGACTCCAGTGGGGTGATGGTTT; ATGGACGATATGACCAATTCCTTCGGGAAGTTCTCAAACTACCCACGGCAGTTTTTGAAGGTCCTTCATT; TGGTTACACAGAACAGTCAGCCAGATCCTGTTTCTCCCAACAGAAAAAAGTCACGTTAAATGGTTTCTTG; GACACGCTTATGTCAGATCCTCCCCCGCAGTGTCTGGTCTGGTTGCCTCTTCTGCATCGACTAGCAAATG; TGGAAAATGTCTTCCATCCGGTTGAGTGTTCCTACTGCCACAGTGAGAGTATGATGGGATTTCGCTACCG; ATGCCAACAGTGTCACAATTACCAGCTCTGTCAGGACTGCTTCTGGAGGGGACATGCCGGTGGTTCTCAT; AGCAACCAGCACCAAATGAAAGAGTACACGTCATGGAAATCACCTGCTAAGAAGCTGACTAATGCATTAA; GCAAGTCCCTGAGCTGTGCTTCCAGCCGTGAACCTTTGCACCCCATGTTCCCAGATCAGCCTGAGAAGCC; ACTCAACTTGGCTCACATCGTTGATACTTGGCCTCCCAGACCTGTAACCAGCATGAACGACACCCTGTTC; TCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTTATTACCAGGAGCATGCTTGAGAGTTCAAACCGGCTTG; ATGAAGAACACAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAGCAGAGTCCTCTTCGTCTCAGCCACC; TCAGCAGAGAAGTGCTCCTGACATCTCTTTCACCATCGATGCGAATAAGCAGCAAAGGCAGCTGATTGCT; GAGCTAGAAAACAAGAACAGAGAAATCTTACAGGAGATCCAGAGACTTCGGCTAGAGCATGAACAAGCTT; CTCAGCCCACGCCAGAGAAGGCACAGCAAAACCCCACCCTGCTGGCAGAACTCCGGCTCCTCAGACAGCG; CAAAGATGAGCTGGAACAGAGAATGTCTGCTCTCCAGGAGAGCCGGAGAGAGCTAATGGTCCAGTTGGAG; GGTCTCATGAAGCTACTAAAGGAAGAAGAACTGAAGCAGGGAGTAAGTTATGTCCCCTACTGCAGGTCTT; AA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032979 unedited; ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGCCAGGAC; TGCGGCGCGGCGCCTCCCCGGGCGGCGGGCGGCGCCTCAGGCCCGCGGGGCGGCGGTAGC; GGCTGCTGCAGGATGTCCGGCCTGGGGGCGCGGGGCCGCGCAGATAAATAGGATTTACCA; ATCCTTGGATGAAGTGCTTGGGAAGTCTTTAAGTGCCATAATCAACTGCCATTTCAAAGA; ATATAGATGGTTTTGAAAAGTTCATGCTGTCCCTTCATTGAATTTTAGAATGATTGAAGA; TAGTGGGAAAAGAGGAAATACCATGGCAGAAAGAAGACAGCTGTTTGCAGAGATGAGGGC; TCAAGATCTGGATCGCATCCGACTCTCCACCTACAGAACAGCATGCAAGCTTAGGTTTGT; TCAGAAGAAATGCAATTTGCACCTGGTGGACATATGGAATGTCATAGAAGCATTGCGGGA; AAATGCTCTGAACAACCTGGACCCAAACACTGAACTCAACGTGTCCCGCTTAGAGGCTGT; GCTCTCCACTATTTTTTACCAGCTCAACAAACGGATGCCAACCACTCACCAAATCCATGT; GGAGCAGTCCATCAGCCTCCTCCTTAACTTCCTGCTTGCAGCGTTTGATCCCGAAGGCCA; TGGTAAAATTTCAGTATTTGCTGTCAAAATGGCTCTAGCCCACCATTGTGTGGAGGCAAG; ATCATGGACANATTAAGATATATTTTCTCAATGATTTCTGACTCCAGTGGGGTGATGGTT; TATGGACGATATGACCAATTCCTTCGGGAGGTCTCAAACTACCACCGCAGTTTTTGAAGG; TCCTCATTTGGTACCAGAACATCAGCCAGATCTGTTCTCCCACGAANAAAGTCCCTTAAT; GGTTCTTTNCACCCTTAGTTATATCTCCCCCGAAGGCTGGCTGGTGCCTTTTGAATTACT; ACAAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032979 unedited; NNAAGATTTTGACTCTGNAACCGCGGCCGCATACNANGATCGNGTTTTTTTTTTTTTTTT; TTAATGTAATACATTATCTTTATTGTCTGACATTACAGATTGATTTTACACTTTCCTTTT; TTACAGACCATGGNGAATGAAGGGTAAAAATGTGTCCAGCATTTTCCAAACATTCTTTGT; TACACACAAACACACATAATGAATAATAGAAAAGAGCATACAGAAGAAATGAAAGATACG; TTGAAATTGGACAGGTTGTGGTTCTAATTGATCCATGGAAATAATGTGAAACTTTTAAAT; AATGCTAGTGAGTGGGATATATTGTCATACATAGCGTGGTACATGCACTTGAAATTAAAG; CTGCTTTTATTTTGCTTCTATTTAATTTAATTTAATTTAATTTAATTTGTTTTTTTTTTG; AGTTTGCTGCTCCCCCTGCTGGCTCTTGAGCCTGACCACAGGTTCAGACAGCTCCAGTTT; AGGCTATAAAGTCACAATGCCCCTATTTCCAGAAAAAAAAAAAAGTATGTAATGACCCTC; TCGTCTCCCGGAGCCGAAAGGCTCTGGTGCTAAGGAAAAAGCTGAGAGTTACTTTTTAAA; AAGAGATTACTGTCAACCAACAGTAAGGATTCTGCTTTGGGGAAGAGCCAAAAAGATGGG; ACGTGAATAGTCAGTTATGGAGTACAGTCTTTCTCTCACAAAATTGAGCACATTTTGGTG; AGCCCATGGGAACAAAGGCTCAATAATTGTGTGATGAAAAGGGATTTAANTGCCTGATTT; TTATAAAAAGAAGTTCCACAGGTTTTTCAGTAAAATGAAACCGAACTGAATACTCACCAG; TGTGAGTGGGGTGTAAAAGTTAACTTCTGAGTGCTGTGGGGTTTTGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_032979
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032979.2, NP_116761.2
• RefSeq Size: 3110 bp
• RefSeq ORF: 1542 bp
• Locus ID: 1837
• UniProt ID: Q9Y4J8
• Cytogenetics: 18q12.1
• Domains: ZnF_ZZ
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (5), also known as gamma transcript, lacks two consecutive in-frame coding exons and multiple exons from the 3' end, and contains an alternate 3' exon, compared to transcript variant 1. This results in a shorter isoform (5) with a distinct C-terminus and missing an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.