AP2S1 (NM_021575) Human Untagged Clone

SKU SC109070

AP2S1 (untagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta

Product Data

• Type: Human Untagged Clone
• Target Symbol: AP2S1
• Synonyms: AP17; CLAPS2; FBH3; FBHOk; HHC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_021575 edited; ATGATCCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGGCCAAGTGGTAC; ATGCAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCATGCCGTGGTCACC; GTCCGAGACGCCAAACACACCAACTTTGTGGAGTTCCGGAACTTTAAGATCATTTACCGC; CGCTATGCTGGCCTCTACTTCTGCATCTGTGTGGATGTCAATGACAACAACCTGGCTTAC; CTGGAGGCCATTCACAACTTCGTGGAGGTCTTAAACGAATATTTCCACAATGTCTGTGAA; CTGGACCTGGTGTTCAACTTCTACAAGGTTTACACGGTCGTGGACGAGATGTTCCTGGCT; GGCGAAATCCGAGAGACCAGCCAGACGAAGGTGCTGAAACAGCTGCTGATGCTACAGTCC; CTGGAGTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021575 unedited; GGGGTTTAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGACC; GGGGCCGCTGGGGTCTGGACGGGGGTCGCCATGATCCGCTTTATCCTCATCCAGAACCGG; GCAGGCAAGACGCGCCTGGCCAAGTGGTACATGCAGTTTGATGATGATGAGAAACAGAAG; CTGATCGAGGAGGTGCATGCCGTGGTCACCGTCCGAGACGCCAAACACACCAACTTTGTG; GAGTTCCGGAACTTTAAGATCATTTACCGCCGCTATGCTGGCCTCTACTTCTGCATCTGT; GTGGATGTCAATGACAACAACCTGGCTTACCTGGAGGCCATTCACAACTTCGTGGAGGTC; TTAAACGAATATTTCCACAATGTCTGTGAACTGGACCTGGTGTTCAACTTCTACAAGGTT; TACACGGTCGTGGACGAGATGTTCCTGGCTGGCGAAATCCGAGAGACCAGCCAGACGAAG; GTGCTGAAACAGCTGCTGATGCTACAGTCCCTGGAGTGAGGGCAGGCGAGCCCCACCCCG; GCCCCGGCCCCTCCTGGACTCGCCTGCTCGCTTCCCCTTCCCAGGCCCGTGGCCAACCCA; GCAGTCCTTCCCTCAGCTGCCTAGGAGGAAGGGACCCAGCTGGGTCTGGGCCACAAGGGA; GGAGACTGCACCCCACTGCCTCTGGGCCCTGGCTGTGGGCAGAGGCCACCGTGTGTGTCC; CGATAACCGTGCCGTTGTCGTGTGATGCCATAAGCGTCTGTGCGTGGAGTTCCCCATAAA; CCTGTGGTCCTGCNNNNAANNNNNNNNNNNCNNNNNTTTTTCNNNCTTTTTCNTNNCNTT; TTTTNNTTTTNTTNTTTTTTTTTTNNTTTTTTTTTTTTTTCTCTTTTTTTTTTTCCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_021575
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021575.2, NP_067586.1
• RefSeq Size: 776 bp
• RefSeq ORF: 315 bp
• Locus ID: 1175
• UniProt ID: P53680
• Cytogenetics: 19q13.32
• Domains: Clat_adaptor_s
• Protein Pathways: Endocytosis, Huntington's disease
• Summary: One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]; Transcript Variant: This variant (AP17delta) contains an alternate 5' terminal exon, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the coding region, compared to variant 3. It encodes isoform AP17delta, which is shorter and has a distinct N-terminus, compared to isoform 3.