DDX21 (NM_004728) Human Untagged Clone

CAT#: SC108813

DDX21 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (DDX21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX21
• Synonyms: GUA; GURDB; RH-II/GU; RH-II/GuA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004728, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGGAAAACTCCGTAGTGACGCTGGTTTGGAATCAGACACCGCAATGAAAAAAGGGGAGACACTGC
  GAAAGCAAACCGAGGAGAAAGAGAAAAAAGAGAAGCCAAAATCTGATAAGACTGAAGAGATAGCAGAAGA
  GGAAGAAACTGTTTTCCCCAAAGCTAAACAAGTTAAAAAGAAAGCAGAGCCTTCTGAAGTTGACATGAAT
  TCTCCTAAATCCAAAAAGGCAAAAAAGAAAGAGGAGCCATCTCAAAATGACATTTCTCCTAAAACCAAAA
  GTTTGAGAAAGAAAAAGGAGCCCATTGAAAAGAAAGTGGTTTCTTCTAAAACCAAAAAAGTGACAAAAAA
  TGAGGAGCCTTCTGAGGAAGAAATAGATGCTCCTAAGCCCAAGAAGATGAAGAAAGAAAAGGAAATGAAT
  GGAGAAACTAGAGAGAAAAGCCCCAAACTGAAGAATGGATTTCCTCATCCTGAACCGGACTGTAACCCCA
  GTGAAGCTGCCAGTGAAGAAAGTAACAGTGAGATAGAGCAGGAAATACCTGTGGAACAAAAAGAAGGCGC
  TTTCTCTAATTTTCCCATATCTGAAGAAACTATTAAACTTCTCAAAGGCCGAGGAGTGACCTTCCTATTT
  CCTATACAAGCAAAGACATTCCATCATGTTTACAGCGGGAAGGACTTAATTGCACAGGCACGGACAGGAA
  CTGGGAAGACATTCTCCTTTGCCATCCCTTTGATTGAGAAACTTCATGGGGAACTGCAAGACAGGAAGAG
  AGGCCGTGCCCCTCAGGTACTGGTTCTTGCACCTACAAGAGAGTTGGCAAATCAAGTAAGCAAAGACTTC
  AGTGACATCACAAAAAAGCTGTCAGTGGCTTGTTTTTATGGTGGAACTCCCTATGGAGGTCAATTTGAAC
  GCATGAGGAATGGGATTGATATCCTGGTTGGAACACCAGGTCGTATCAAAGACCACATACAGAATGGCAA
  ACTAGATCTCACCAAACTTAAGCATGTTGTCCTGGATGAAGTGGACCAGATGTTGGATATGGGATTTGCT
  GATCAAGTGGAAGAGATTTTAAGTGTGGCATACAAGAAAGATTCTGAAGACAATCCCCAAACATTGCTTT
  TTTCTGCAACTTGCCCTCATTGGGTATTTAATGTTGCCAAGAAATACATGAAATCTACATATGAACAGGT
  GGACCTGATTGGTAAAAAGACTCAGAAAACGGCAATAACTGTGGAGCATCTGGCTATTAAGTGCCACTGG
  ACTCAGAGGGCAGCAGTTATTGGGGATGTCATCCGAGTATATAGTGGTCATCAAGGACGCACTATCATCT
  TTTGTGAAACCAAGAAAGAAGCCCAGGAGCTGTCCCAGAATTCAGCTATAAAGCAGGATGCTCAGTCCTT
  GCATGGAGACATTCCACAGAAGCAAAGGGAAATCACCCTGAAAGGTTTTAGAAATGGTAGTTTTGGAGTT
  TTGGTGGCAACCAATGTTGCTGCACGTGGGTTAGACATCCCTGAGGTTGATTTGGTTATACAAAGCTCTC
  CACCAAAGGATGTAGAGTCCTACATTCATCGATCCGGGCGGACAGGCAGAGCTGGAAGGACGGGGGTGTG
  CATCTGCTTTTATCAGCACAAGGAAGAATATCAGTTAGTACAAGTGGAGCAAAAAGCGGGAATTAAGTTC
  AAACGAATAGGTGTTCCTTCTGCAACAGAAATAATAAAAGCTTCCAGCAAAGATGCCATCAGGCTTTTGG
  ATTCCGTGCCTCCCACTGCCATTAGTCACTTCAAACAATCAGCTGAGAAGCTGATAGAGGAGAAGGGAGC
  TGTGGAAGCTCTGGCAGCAGCACTGGCCCATATTTCAGGTGCCACGTCCGTAGACCAGCGCTCCTTGATC
  AACTCAAATGTGGGTTTTGTGACCATGATCTTGCAGTGCTCAATTGAAATGCCAAATATTAGTTATGCTT
  GGAAAGAACTTAAAGAGCAGCTGGGCGAGGAGATTGATTCCAAAGTGAAGGGAATGGTTTTTCTCAAAGG
  AAAGCTGGGTGTTTGCTTTGATGTACCTACCGCATCAGTAACAGAAATACAGGAGAAATGGCATGATTCA
  CGACGCTGGCAGCTCTCTGTGGCCACAGAGCAACCAGAACTGGAAGGACCACGGGAAGGATATGGAGGCT
  TCAGGGGACAGCGGGAAGGCAGTCGAGGCTTCAGGGGACAGCGGGACGGAAACAGAAGATTCAGAGGACA
  GCGGGAAGGCAGTAGAGGCCCGAGAGGACAGCGATCAGGAGGTGGCAACAAAAGTAACAGATCCCAAAAC
  AAAGGCCAGAAGCGGAGTTTCAGTAAAGCATTTGGTCAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004728 unedited
  GTCAAATGTTGTATACGACTCCTATAGGCGGCCGCGAAATTCTCACGAGGTTGAGAAGAC
  CGGTCGGCCTGGGCAACCTGCGCTGAAATGCCGGGAAAACTCCGTAGTGACGCTGGTTTG
  GAATCAGACACCGCAATGAAAAAAGGGGAGACACTGCGAAAGCAAACCGAGGAGAAAGAG
  AAAAAAGAGAAGCCAAAATCTGATAAGACTGAAGAGATAGCAAAAGAGGAAGAAACTGTT
  TTCCCCAAAGCTAAACAAGTTAAAAAGAAAGCAGAGCCTTCTGAAGTTGACATGAATTCT
  CCTAAATCCAAAAAGGCAAAAAAGAAAGAGGAGCCATCTCAAAATGACATTTCTCCTAAA
  ACCAAAAGTTTGAGAAAGAAAAAGGAGCCCATTGAAAAGAAAGTGGTTTCTTCTAAAACC
  AAAAAAGTGACAAAAAATGAGGAGCCTTCTGAGGAAGAAATAGATGCTCCTAAGCCCAAG
  AAGATGAAGAAAGAAAAGGAAATGAATGGAGAAACTAGAGAGAAAAGCCCCAAACTGAAG
  AATGGATTTCCTCATCCTGAACCGGACTGTAACCCCAGTGAAGCTGCCAGTGAAGAAAGT
  AACAGTGAGATAGAGCAGGAAATACCTGTGGAACAAAAAGAAGGCGCTTTCTCTAATTTT
  CCCATANTCTAAAGAAACTATTAAACTTCTCAAGGGCCGAGGAGTGACCTTCCTATTTCC
  TATACNAGCAAAGACATTCCATCATGTTTACAGCGGNGAAAGACTTAATTGCACAGGCAC
  GGACAGGAACTGGGGAAGACATTCTNCTTTGCCATCCTTTTGATTGAGAACTNCATGGNG
  AACTGCAGACAGGAAGAANAGCCGTGCCCCTCAGGTACTGGGTCTTGCACTACNNAAAAA
  GTTGCAAATCAGTAGCAAGAACTTCGTGACTACAAAAAACTGCATGGGCTGTTTTATAGG
  GG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004728 unedited
  TTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTACAAACT
  CTTGAGCAATGCTAATCTGCGCCCCTTACTCCCTTAAGTCCTTCTTGGTAAATAATGTTA
  ATCTTCCAATAGGAAGAAGTGGAGTACATTACCATTTAAGCACCATTTATCCAGCCTACT
  TACAAATAAAGCTATGGAGCCACCTTATACATGTGAAATTCCTTAAAACCCTGGCTTTCT
  ATTAAAATGTACTTTTATATATACTATCTATGAAGAATTCACTAAAGCATGAATCACCTT
  ATAATGAGAAGCTAAAAATGTATCAAAACGAACATAAGTATAGGTAATCCACATCAAACA
  TACTACATCTTCCAAGTCTAGAGCATACACTGGTATAAACTGTATTACAACCCAGATTAG
  TTTGAAATCTTGTTTCAAAACATTGCTCAGTATTAAGTCTCAGTAGACAAATAATAGGAC
  CACATGAGAAACTGTTCGGCAGGTGGCTGAGGAAACCTTAACTTCCAAAGGCTCAAAGTG
  GTCCTCCAGAGACTGTTACACTCCCTTAGGTATTTATTTCAGGGAAGGACACTATTAAGG
  GACACTTTTGAGTATAAAGACAGGTGAACTCACAAAGTATAGGCAGATACATGCTTGATT
  TTATCTTCTAATCTACCAGATATACATTAGAAATAAAATGGTATGAATTCATACACCCTT
  TCAAAAGGAAAAACTGATGAAGTAACAATAAAGTTTTAAATGATCATGATCAGATGAAAT
  AATTTAATGAAGCTTGTCTGTGTCTGAAAAACACAAGTACTTGGCAAGGGTCAAAGGGAG
  CCCAAGCGCTTTTAACTTGCATGAAAAAAAGTTAAGTCTATTTGCCAACTCATTCTTTTT
  GGCATAAAAACTTCATTTCCATTATGACCAAGCTTTATGAAACCCCCTTTGGGCTTGTTT
  GGAATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004728
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004728.2, NP_004719.2
• RefSeq Size: 4153 bp
• RefSeq ORF: 2352 bp
• Locus ID: 9188
• UniProt ID: Q9NR30
• Cytogenetics: 10q22.1
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.