ENPP1 (NM_006208) Human Untagged Clone

SKU SC108777

ENPP1 (untagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ENPP1
• Synonyms: ARHR2; COLED; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006208, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCGGGAGGGCC; CGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGGGGACCCGCAGGCGGCCGC; GTCCTTGCTGGCCCCTATGGACGTGGGGGAGGAGCCGCTGGAGAAGGCGGCGCGCGCCCGCACTGCCAAG; GACCCCAACACCTATAAAGTACTCTCGCTGGTATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTA; TATTTGGGTTGAAACCAAGCTGTGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATT; TGGGAACTGTCGCTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGC; ATAGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAGAAGCCTCT; GTGCCTGTTCAGATGACTGCAAGGACAAGGGCGACTGCTGCATCAACTACAGTTCTGTGTGTCAAGGTGA; GAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCCACAGTGCCCAGCAGGGTTTGAAACGCCT; CCTACCCTCTTATTTTCTTTGGATGGATTCAGGGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTG; TTATTAGCAAACTAAAAAAATGTGGAACATATACTAAAAACATGAGACCGGTATATCCAACAAAAACTTT; CCCCAATCACTACAGCATTGTCACCGGATTGTATCCAGAATCTCATGGCATAATCGACAATAAAATGTAT; GATCCCAAAATGAATGCTTCCTTTTCACTTAAAAGTAAAGAGAAATTTAATCCTGAGTGGTACAAAGGAG; AACCAATTTGGGTCACAGCTAAGTATCAAGGCCTCAAGTCTGGCACATTTTTCTGGCCAGGATCAGATGT; GGAAATTAACGGAATTTTCCCAGACATCTATAAAATGTATAATGGTTCAGTACCATTTGAAGAAAGGATT; TTAGCTGTTCTTCAGTGGCTACAGCTTCCTAAAGATGAAAGACCACACTTTTACACTCTGTATTTAGAAG; AACCAGATTCTTCAGGTCATTCATATGGACCAGTCAGCAGTGAAGTCATCAAAGCCTTGCAGAGGGTTGA; TGGTATGGTTGGTATGCTGATGGATGGTCTGAAAGAGCTGAACTTGCACAGATGCCTGAACCTCATCCTT; ATTTCAGATCATGGCATGGAACAAGGCAGTTGTAAGAAATACATATATCTGAATAAATATTTGGGGGATG; TTAAAAATATTAAAGTTATCTATGGACCTGCAGCTCGATTGAGACCCTCTGATGTCCCAGATAAATACTA; TTCATTTAACTATGAAGGCATTGCCCGAAATCTTTCTTGCCGGGAACCAAACCAGCACTTCAAACCTTAC; CTGAAACATTTCTTACCTAAGCGTTTGCACTTTGCTAAGAGTGATAGAATTGAGCCCTTGACATTCTATT; TGGACCCTCAGTGGCAACTTGCATTGAATCCCTCAGAAAGGAAATATTGTGGAAGTGGATTTCATGGCTC; TGACAATGTATTTTCAAATATGCAAGCCCTCTTTGTTGGCTATGGACCTGGATTCAAGCATGGCATTGAG; GCTGACACCTTTGAAAACATTGAAGTCTATAACTTAATGTGTGATTTACTGAATTTGACACCGGCTCCTA; ATAACGGAACTCATGGAAGTCTTAACCACCTTCTAAAGAATCCTGTTTATACGCCAAAGCATCCCAAAGA; AGTGCACCCCCTGGTACAGTGCCCCTTCACAAGAAACCCCAGAGATAACCTTGGCTGCTCATGTAACCCT; TCGATTTTGCCGATTGAGGATTTTCAAACACAGTTCAATCTGACTGTGGCAGAAGAGAAGATTATTAAGC; ATGAAACTTTACCCTATGGAAGACCTAGAGTTCTCCAGAAGGAAAACACCATCTGTCTTCTTTCCCAGCA; CCAGTTTATGAGTGGATACAGCCAAGACATCTTAATGCCCCTTTGGACATCCTATACCGTGGACAGAAAT; GACAGTTTCTCTACGGAAGACTTCTCCAACTGTCTGTACCAGGACTTTAGAATTCCTCTTAGTCCTGTCC; ATAAATGTTCATTTTATAAAAATAACACCAAAGTGAGTTACGGGTTCCTCTCCCCACCACAACTAAATAA; AAATTCAAGTGGAATATATTCTGAAGCTTTGCTTACTACAAATATAGTGCCAATGTACCAGAGTTTTCAA; GTTATATGGCGCTACTTTCATGACACCCTACTGCGAAAGTATGCTGAAGAAAGAAATGGTGTCAATGTCG; TCAGTGGTCCTGTGTTTGACTTTGATTATGATGGACGTTGTGATTCCTTAGAGAATCTGAGGCAAAAAAG; AAGAGTCATCCGTAACCAAGAAATTTTGATTCCAACTCACTTCTTTATTGTGCTAACAAGCTGTAAAGAT; ACATCTCAGACGCCTTTGCACTGTGAAAACCTAGACACCTTAGCTTTCATTTTGCCTCACAGGACTGATA; ACAGCGAGAGCTGTGTGCATGGGAAGCATGACTCCTCATGGGTTGAAGAATTGTTAATGTTACACAGAGC; ACGGATCACAGATGTTGAGCACATCACTGGACTCAGCTTCTATCAACAAAGAAAAGAGCCAGTTTCAGAC; ATTTTAAAGTTGAAAACACATTTGCCAACCTTTAGCCAAGAAGACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006208 unedited; GGCTGGGATTTGTAAACGACTTATATAGGCGGCCGCGCATTCGGCACGAGGGCCACGATG; GTAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCG; GGAGGGCCCGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGG; GGACCCGCAGGCGGCCGCGTCCTTGCTGGCCCTTATGGACGTGGGGGAGGAGCCGCTGGA; GAAGGCGGCGCGCGCCCGCACTGCCAAGGACCCCAACACCTATAAAGTACTCTCGCTGGT; ATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTATATTTGGGTTGAAACCAAGCTG; TGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATTTGGGAACTGTCG; CTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGCAT; AGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAG; AAGCCTCTGTGCCTGTTCAGATGACTGCAAGGACCAGGGCGACTGCTGCATCAACTACAG; TTCTGTGTGTCAAGGTGAGAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCC; ACAGTGCCCAGCAGGGTTTGAAACGCCTCCTACCCTCTTATTTTCTTTGGATGGATTCAG; GGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTGTTATTAGCAAACTAAANAAATG; TGGAACATATACTANNAACATGAGACCGGTATATCCAACAAAAACTTTCCCCAATCACTA; CAGCATTGTCACCGGGATGTATCCAGAATCTCATGGCATAATCGACAATAAATGTATGAT; CCCCAAATGAATGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006208 unedited; AGCTTTGNAACCGCGGCCGCAATCTANAAGTCGGTTTTTTTTTTTTTTTTTGATTACAAT; GAAAAGAAATGAAACTCCAAGTTAAAAGATATCCAGAACCACTTTAAAATTCTCATAAAG; ATAGTCACATAACATGGGCTACCCCAACCCTACTATAAGTCTACTGTTTGCCCAATGCCA; TCAAGGTTTATTACCAATAGTAAGAAAGATTCAAATATCTGGTATCCAGACAATGTTCAC; AGCTACTTCTCCTTTAAGAGAAGCAGAAAGGCAGTTTTAGGTGTGGTCTGTCTTTATTCC; CCCGAACTTGATCAATGTATACAATGCTTACACATTCAAATATGTGCAAGATTCAACAGG; AACACTCTCCACTGCTGTGCTGAGTACGCAAGTTTCCCTGAGATGTCCGCATCACCGAGG; GCTCCGTTCTAACTCTGGTCGACAGTTTCTGAACAATGCAATAGTGTAGCTAGAGGACTA; TATAAAATATAAGGTTCTCTCAAAAAGATTCATGGTGTTTGGGGATAAAAAACATATCAG; TCTTCTTGGCTAAAGGTTGGCAAATGTGTTTTCAACTTTAAAATGTCTGAAACTGGCTCT; TTTCTTTGTTGATAGAAGCTGAGTCCAGTGATGTGCTCAACATCTGTGATCCGTGCTCTG; TGTAACATTAACAATTCTTCAACCCATGAGGAGTCATGCTTCCCATGCACACAGCTCTCG; CTGTTATCAGTCCTGTGAGGCAAAATGAAAGCTAAGGTGTCTTAGGTTTTCACAGTGCAA; AAGCGTCTGAGATGTATNCTTACAGCTTGTTAGCACATAAAGAAATGAGTTTGGATCAAA; ATTCCTGGTTACGGATGACTCTNCTTTTTTGCTCAGATCTCTAAGGATCACACGTNCATC; ATATCAAGTCAAACACAGGACACTGACGACATTGACACCATTTCTTTCTCANCTACTTTT; TCCATAGGGTGTATGAAAGTACGCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006208
• Insert Size: 3160 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006208.1, NP_006199.1
• RefSeq Size: 7442 bp
• RefSeq ORF: 2622 bp
• Locus ID: 5167
• UniProt ID: P22413
• Cytogenetics: 6q23.2
• Domains: Endonuclease, Phosphodiest, SO
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Pantothenate and CoA biosynthesis, Purine metabolism, Riboflavin metabolism, Starch and sucrose metabolism
• Summary: This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]