IL10RA (NM_001558) Human Untagged Clone

SKU SC108765

IL10RA (untagged)-Human interleukin 10 receptor, alpha (IL10RA), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IL10RA
• Synonyms: CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108765 sequence for NM_001558 edited (data generated by NextGen Sequencing); ATGCTGCCGTGCCTCGTAGTGCTGCTGGCGGCGCTCCTCAGCCTCCGTCTTGGCTCAGAC; GCTCATGGGACAGAGCTGCCCAGCCCTCCGTCTGTGTGGTTTGAAGCAGAATTTTTCCAC; CACATCCTCCACTGGACACCCATCCCAAATCAGTCTGAAAGTACCTGCTATGAAGTGGCG; CTCCTGAGGTATGGAATAGAGTCCTGGAACTCCATCTCCAACTGTAGCCAGACCCTGTCC; TATGACCTTACCGCAGTGACCTTGGACCTGTACCACAGCAATGGCTACCGGGCCAGAGTG; CGGGCTGTGGACGGCAGCCGGCACTCCAACTGGACCGTCACCAACACCCGCTTCTCTGTG; GATGAAGTGACTCTGACAGTTGGCAGTGTGAACCTAGAGATCCACAATGGCTTCATCCTC; GGGAAGATTCAGCTACCCAGGCCCAAGATGGCCCCCGCGAATGACACATATGAAAGCATC; TTCAGTCACTTCCGAGAGTATGAGATTGCCATTCGCAAGGTGCCGGGAAACTTCACGTTC; ACACACAAGAAAGTAAAACATGAAAACTTCAGCCTCCTAACCTCTGGAGAAGTGGGAGAG; TTCTGTGTCCAGGTGAAACCATCTGTCGCTTCCCGAAGTAACAAGGGGATGTGGTCTAAA; GAGGAGTGCATCTCCCTCACCAGGCAGTATTTCACCGTGACCAACGTCATCATCTTCTTT; GCCTTTGTCCTGCTGCTCTCCGGAGCCCTCGCCTACTGCCTGGCCCTCCAGCTGTATGTG; CGGCGCCGAAAGAAGCTACCCAGTGTCCTGCTCTTCAAGAAGCCCAGCCCCTTCATCTTC; ATCAGCCAGCGTCCCTCCCCAGAGACCCAAGACACCATCCACCCGCTTGATGAGGAGGCC; TTTTTGAAGGTGTCCCCAGAGCTGAAGAACTTGGACCTGCACGGCAGCACAGACAGTGGC; TTTGGCAGCACCAAGCCATCCCTGCAGACTGAAGAGCCCCAGTTCCTCCTCCCTGACCCT; CACCCCCAGGCTGACAGAACGCTGGGAAACGGGGAGCCCCCTGTGCTGGGGGACAGCTGC; AGTAGTGGCAGCAGCAATAGCACAGACAGCGGGATCTGCCTGCAGGAGCCCAGCCTGAGC; CCCAGCACAGGGCCCACCTGGGAGCAACAGGTGGGGAGCAACAGCAGGGGCCAGGATGAC; AGTGGCATTGACTTAGTTCAAAACTCTGAGGGCCGGGCTGGGGACACACAGGGTGGCTCG; GCCTTGGGCCACCACAGTCCCCCGGAGCCTGAGGTGCCTGGGGAAGAAGACCCAGCTGCT; GTGGCATTCCAGGGTTACCTGAGGCAGACCAGATGTGCTGAAGAGAAGGCAACCAAGACA; GGCTGCCTGGAGGAAGAATCGCCCTTGACAGATGGCCTTGGCCCCAAATTCGGGAGATGC; CTGGTTGATGAGGCAGGCTTGCATCCACCAGCCCTGGCCAAGGGCTATTTGAAACAGGAT; CCTCTAGAAATGACTCTGGCTTCCTCAGGGGCCCCAACGGGACAGTGGAACCAGCCCACT; GAGGAATGGTCACTCCTGGCCTTGAGCAGCTGCAGTGACCTGGGAATATCTGACTGGAGC; TTTGCCCATGACCTTGCCCCTCTAGGCTGTGTGGCAGCCCCAGGTGGTCTCCTGGGCAGC; TTTAACTCAGACCTGGTCACCCTGCCCCTCATCTCTAGCCTGCAGTCAAGTGAGTGA; ; Clone variation with respect to NM_001558.3; 459 a=>g;1051 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001558 unedited; NNCCTTCGGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGCGCA; GGCCGGCTCCGCTCCGGCCCCGGACGATGCGGCGCGCCCAGGATGCTGCCGTGCCTCGTA; GTGCTGCTGGCGGCGCTCCTCAGCCTCCGTCTTGGCTCAGACGCTCATGGGACAGAGCTG; CCCAGCCCTCCGTCTGTGTGGTTTGAAGCAGAATTTTTCCACCACATCCTCCACTGGACA; CCCATCCCAAATCAGTCTGAAAGTACCTGCTATGAAGTGGCGCTCCTGAGGTATGGAATA; GAGTCCTGGAACTCCATCTCCAACTGTAGCCAGACCCTGTCCTATGACCTTACCGCAGTG; ACCTTGGACCTGTACCACAGCAATGGCTACCGGGCCAGAGTGCGGGCTGTGGACGGCAGC; CGGCACTCCAACTGGACCGTCACCAACACCCGCTTCTCTGTGGATGAAGTGACTCTGACA; GTTGGCAGTGTGAACCTAGAGATCCACAATGGCTTCATCCTCGGGAAGATTCAGCTACCC; AGGCCCAAGATGGCCCCCGCGAATGACACATATGAAAGCATCTTCAGTCACTTCCGAGAG; TATGAGATTGCCATTCGCAAGGTGCCGGGAAACTTCACGTTCACACACAAGANAGTAAAA; CATGAAAACTTCAGCCTCCTAACCTCTGGAGAAAGTGGGAGAAGTTCTGTGTCCAGGTGA; AACCATCTGTCGCTTCCCGAAGTACAGGNNGATGTGGTCTAAGAGGAGTGCATCTCCCTC; ACCAGCAGTATTTCACCGTGACCAACGTCATCATCTTCTTTCCCTTGTCCTGCTGCTCTC; CGGAGCCCTCGCTACTGCCTGGCCTNCAGCTGTATGTGCCGCGCCGAAGAGCTACCCAGT; GTCTGCTCTTCAGAAGCCAGCCCTTCTCTTCATAACCACGTCCTTCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001558 unedited; GGGAAATAGCTTGNCCGCGGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTGG; TCTTGACAATTTATTGGAATAAAAAACATGTATCACTTATTGACAGAGCTTTGTGTTCAA; GGCACATTCATACCCATTTCCTCAGCAGAACCTCACATCCCTATGAATTAGACAAGTCAG; TCATTATTCTGCAGATGAGGAAACTGAGGCTCCAAGAGGATAAGTGACTTCTCCAAGGTC; ATACCACTGGAAACAGCAAAGTCAGAGCTAGAATTTCGGGGCTCCTGAGATATCCAGAAT; TCTTTCACTGTGCAATGCTGCCTCTCCAATAAATAAATGAACAAAATAAATAAATAAAGC; TTTCAAGGGAACCCTGAGGAATCCTCCCTCAAGCCTGCCTCCAGAGGGAGTTCAGTTACC; AGACTGGCACCTAAGAGTACTTTGGCAGAGGGGTGGGGGTCATGGCTGGATTCCCTGGGC; CAGAATATCTGTTTTCAGAATGCTCCAGAGAAAGGCAGGGCTGAGATGACTCTGGATGGC; ACGCACAGGCCTGTCCCCTCACAGCTTCCATGCCTGTGGACTGAACTGCCTTTGTTTGAA; GGCAGAGTCCAAATGGCCTATGGGGAGGCAGGGCCCATAATGCCCCCTTCTGGGGCTGTG; TCTGATACTGTCTTGGCATGACACCATGGACTCCCATTGGATCTGTTAGCCTCTGGCATA; AGAAACAAGCCAGCTTGGTAGTTCACGGACAGTATCTGCATGCTCCCAGGACAGCTTGGC; TAATACCCAGCAGACATGGAGGGTCCCCTAAAGTGGAGGTCCTTATTTCACAGATGGGAG; ATTGAGGCCCTCTGACAGTGGGTAGTCCAAATCACCCAGCTGACA;
• Restriction Sites: NotI-NotI
• ACCN: NM_001558
• Insert Size: 1737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001558.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001558.2, NP_001549.1
• RefSeq Size: 3649 bp
• RefSeq ORF: 1737 bp
• Locus ID: 3587
• UniProt ID: Q13651
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Summary: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]; Transcript Variant: This variant (1) is the protein-coding transcript.