PNPO (NM_018129) Human Untagged Clone

SKU SC108655

PNPO (untagged)-Human pyridoxamine 5'-phosphate oxidase (PNPO)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PNPO
• Synonyms: HEL-S-302; PDXPO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108655 sequence for NM_018129 edited (data generated by NextGen Sequencing); ATGACGTGCTGGCTGCGGGGCGTCACGGCGACGTTCGGGCGACCTGCCGAGTGGCCAGGC; TACCTCAGTCACCTGTGTGGTCGCAGTGCTGCCATGGACCTGGGACCCATGCGCAAGAGT; TACCGCGGGGACCGAGAGGCATTTGAGGAGACTCATCTGACCTCCCTTGACCCAGTGAAA; CAGTTTGCTGCCTGGTTTGAGGAGGCTGTTCAGTGTCCTGACATAGGGGAAGCCAATGCC; ATGTGTCTGGCTACCTGCACCAGAGATGGAAAACCCTCTGCTCGCATGTTGCTGCTGAAG; GGCTTCGGGAAAGATGGCTTCCGCTTCTTCACTAACTTCGAGAGTCAAAAAGGAAAAGAG; CTGGACTCTAATCCCTTTGCTTCCCTTGTCTTCTACTGGGAGCCACTTAACCGTCAGGTG; CGTGTGGAAGGCCCTGTGAAGAAACTGCCTGAGGAGGAGGCTGAGTGCTACTTCCACTCC; CGCCCCAAGAGCAGCCAGATTGGGGCTGTGGTCAGCCACCAGAGTTCTGTGATCCCTGAT; CGGGAGTATCTAAGAAAGAAAAATGAGGAACTGGAACAGCTCTACCAGGATCAAGAGGTG; CCCAAGCCAAAATCCTGGGGTGGCTATGTCCTGTACCCTCAGGTGATGGAGTTCTGGCAA; GGTCAAACCAACCGCCTGCATGACCGGATAGTCTTTCGGCGGGGCCTACCCACAGGAGAT; TCCCCTTTGGGGCCCATGACCCACCGCGGGGAGGAAGACTGGCTCTATGAGAGACTTGCA; CCTTAA; ; Clone variation with respect to NM_018129.3; 347 g=>a;552 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018129 unedited; GGTCACCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCTCAA; AGGAACCCAGTGCCGGGCCACAGCCGGGTCACGTGGCCGGCGGCCCCCCATGACGTGCTG; GCTGCGGGGCGTCACGGCGACGTTCGGGCGACCTGCCGAGTGGCCAGGCTACCTCAATCA; CCTGTGTGGTCGCAGTGCTGCCATGGACCTGGGACCCATGCGCAAGAGTTACCGCGGGGA; CCGAGAGGCATTTGAGGAGACTCATCTGACCTCCCTTGACCCACTGAAACAGTTTGCTGC; CTGGTTTGAGGAGGCTGTTCAGTGTCCTGACATAGGGGAAGCCAATGCCATGTGTCTGGC; TACCTGCACCAGAGATGGAAAACCCTCTGCTCGCATGTTGCTGCTGAAGGGCTTCGGGAA; AGATGGCTTCCGCTTCTTCACTAACTTCGAGAGTCAAAAAGGAAAAGAGCTGGACTCTAA; TCCCTTTGCTTCCCTTGTCTTCTACTGGGAGCCACTTAACCGTCAGGTGCGTGTGGAAGG; CCCTGTGAAGAAACTGCCTGAGGAGGAGGCTGAGTGCTACTTCCACTCCCGCCCCAAGAG; CANCCAAATCGGGGCTGTGGTCAGCCACCAAAGTTCTGTGATCCCTGATCGGGAGTATCT; AATAAAGAAACATGAGGAACTGGAACAGCTCTACCACGATCACAGAGTGCCCAAGCCAAA; ATCCTGTGGTGGCTATGTCCTGTCCCCTCACGTGATGGAGTTCTGGCCAGTTCAAACCTC; CCGCCTGCATGACCGGATAATCTTTCCGCGGTGCCTACCCCAGGAAATTCCTCTTTGGGG; CCCATTGACCACCCTGGGGAGAACACTGGCTCTTAGAGAGACTTGCCCCTAACTTTGGGC; ACTGCTTGCCCCAATGGAGCTTGGCTCGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018129 unedited; GGGGGGCGGGGTCCGCACAACCACACTAAACAAAAGGANGTTGGTGAAACTATAAACCCT; TTTTTGTTGGAAATGTCTGCCTCACATACACAGCAGTTTCCATCTTAAAACATACATAGT; AAAGCTCGATTAAAATCTTCCTGTCCTATTGGTACCAGCACATGAAGCCCTTCTACAAAA; TTCCTGACGGACTGGGAATAAAAATTCCTAGTGACAGCCCACTCCTTCTCAGGCAGGTGC; GATTGCCTGAAATCTCTCCCAATATCGACATGAAACCTGCTTCCCTGTAACTCCCCTGTA; ATTATGTGGGTCCCTTGTAGCCACAGAAAAGGCAGTAATCAGTATGGGAAGTGCTTTAAA; AACTTTCTATCCCGGTCAAGCGTGGTGGCTCATGTCTGTAATCCCAGCACTTTGGGAGGT; CCAGGTGGGCATTATCACTTTATGTCATGAGTTGGAGACCACACTGGTCACCATAATGAA; ACCCTCGTCTCCTGGGTGCGGTGGCTAATGCCTGTAATTCCAACCCTTTCGGTAGGCTGA; GGTGGGCGGATCACATGGTCAAAAGATGGATACATCCTGGCCTACATGGTGAAACCTCTT; TTCTACTAAACATACAACATTTAACTGGTTCTCGTGGCTTTTGTCCTTTATTTCTATCTA; CTTGGTGATGTCTTGGCCGCGCATAATTGCTTTGAACACTGGCGAGGCATACGTTGCCAT; CGATCTCCCCTACACCCCTTTGTACTCTCCCTCTGGGAGGACAGACAGTAAACTTCTTCT; TTTTAAATAATAAGAATTTGTACACACTATTATTTATAACTTTCCTTTCTTCCCCTCATT; CCTTTTTCTTACCTTTGCTCCCCCCTCACTAAATTTCTTTCTTCCCTTTCTCTATTATAC; TTACCCCCACATTGCCACCTCCTCTCCTGTTATATTTGACCTCTTCTAAGCGCGTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_018129
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018129.1, NP_060599.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 786 bp
• Locus ID: 55163
• UniProt ID: Q9NVS9
• Cytogenetics: 17q21.32
• Domains: Pyridox_oxidase
• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism
• Summary: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]