RTN3 (NM_006054) Human Untagged Clone

SKU SC108563

RTN3 (untagged)-Human reticulon 3 (RTN3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RTN3
• Synonyms: ASYIP; HAP; NSPL2; NSPLII; RTN3-A1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108563 sequence for NM_006054 edited (data generated by NextGen Sequencing); ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCC; GAGCCGTCCGCGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAG; AGCTGCAGCTCCTCCTGTGCGGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACT; GGGTTTGTCTTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGTGTCATC; AGTGTGGTTTCTTACCTCATCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGGATCTAC; AAGTCCGTCATCCAAGCTGTACAGAAGTCAGAAGAAGGCCATCCATTCAAAGCCTACCTG; GACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAATTACATGAATGCTGCCATGGTG; CACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAAGATCTGGTTGAC; TCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACGGA; ATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGTGTCCCGATTGTCTATGAGAAG; TACAAGACCCAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACCAAGTCAATTGTT; GAAAAGATCCAAGCAAAACTCCCTGGAATCGCCAAAAAAAAGGCAGAATAA; ; Clone variation with respect to NM_006054.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006054 unedited; GACCTTGGATTTTGTAACCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGGT; CAGTCAGTCTGTCGGAGTCTGGTCCTCGGACAGGCGGAGTAAAGGGACTTGAGCGAGCCA; GTTGCCGGATTATTCTATTTCCCCTCCCTCTCTCCCGCCCCGTATCTCTTTTCACCCTTC; TCCCACCCTCGCTCGCGTAGCCATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCAT; CTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCGCGCCCGGCGGCGGCGGGAGCCCAGGAGC; CTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGTGCGGTGCACGATCTGATTTT; CTGGAGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATCATGCTGCTTTC; CCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTCATCCTGGCTCTTCTCTCTGT; CACCATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGTCAGAAGAAGG; CCATCCATTCAAAGCCTACCTGGACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAA; TTACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTT; TCTGGTAGAAGATCTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTA; TGTTGGTGCTGTTTTTAACGGAATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAG; TGTCCCGATTGTCTATGAGAAGTACCAGACCCAGATTGATCACTATGTTGGCATCGCCCG; AGATCAGACCAAGTCAATTGNTGAAAAGATCCCAGCANNACTCCTGGNATCGCCAAAAAA; AGGCAGNNATAGTACATGGAAACCCAGAATGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006054 unedited; AGCTTGTACGCGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGGTTTTTTT; TTTTTTATTAATACCACATCAATTTGAAATTTTACAGGAACCAAGATTCAAGCTCCTTAG; GGGCTACTGTATTTTTATGTTGCACGCACAGACACGTACACACAGTTTCATTAGTAATTT; TTCATCTATAGGTTTTTCTTAAAAAAAAAAAAAATCCTGTGTGGCACAAAGATCTCTTCA; ATTATCTAAACACAGCAGCAACAAAAGAAAAGAACTGGATGTGGCACTTCCACTGCAAAA; GGTCCTTTGGAGGGGAAGCCCAAAAAGACAATCACTGACTTCCTGGGAGGGCAAAGGATG; AGGATGGAAAATGCACTTCCTACTAGGACATGAAACCAGTTAAAACACTCAAAGGGGGCT; AAGAAAATAAGATGGAGTAAGGCAAGGGTACCTTTTACCCATCCAAATTCCTAACTAATT; TTTCCAAGACACGTAGACAATTTCCTTCCACTACTTTTTGCAGCTGCCGTACTTTGGGAC; CATCAAATATCATAGCCCTTACTTTAAACTCGCCAGTGCATATTCCCTCTGAAGGATCAT; GACTATGAGTGAGAACCTGAAGGAACTCACAGATGATTTTTCTTTCAAGTTCGCTGAATT; CTCATATTGAAGGTGAAGAAAAAAACTTGCCCCCAAATGGGAAATCGAGATTTGAATCTC; GGGAAAAGATCAAGAAAAAAAGGCTTCTGTATTTCACCTCTTGAGAAATGTTCCCCCCAG; ACTGAGATAGGATCCCAAAAACTCATTAACTGGAGGAGCCACATGCACGGTTATTTTTTT; CTCTAACCAGTTTACAGCAGTGCACAGCAGTTACAGACATTTATTTTTAGTAAAAAAGTT; CACCCTTTTTTTTAACTTGAGGTGGCCAGGGAAAGGTAGAAACCCATTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_006054
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006054.2, NP_006045.1
• RefSeq Size: 2583 bp
• RefSeq ORF: 711 bp
• Locus ID: 10313
• UniProt ID: O95197
• Cytogenetics: 11q13.1
• Domains: Reticulon
• Protein Families: Transmembrane
• Summary: This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012]; Transcript Variant: This variant (1) encodes isoform a.