NISCH (NM_007184) Human Untagged Clone

SKU SC108496

NISCH (untagged)-Human nischarin (NISCH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NISCH
• Synonyms: hIRAS; I-1; IR1; IRAS
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007184 edited; GAATTCGGCACGAGGGTGGCGGCGGAGACCCGAACATGGCGACCGCGCGCACCTTCGGGC; CCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCTCGGAGCTTGTGGACA; CTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAAGCACC; GCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAA; ACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGG; AGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCA; GAGTACTGGCCCACTTCTTGCATTTTCACTTCTATGAGATAAATGGCATCACCGCGGCAC; TGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTCCTGGGGGCCGGCGAGGTCTTTGCCA; TTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGGGAAAGCCCACGTGCG; CCAGTGGGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCTTAAGT; ACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGGACCAGCAACATTCAGGAGCAGCTCC; TGCCGTTCGACCTATCAATATTCAAGTCCCTGCATCAGGTGGAGATAAGTCACTGTGATG; CTAAGCACATCAGAGGGCTGGTCGCATCGAAGCCCACCTTAGCCACGCTGAGTGTCCGCT; TCTCAGCAACCTCGATGAAGGAAGTCCTTGTTCCTGAAGCCTCAGAATTTGATGAGTGGG; AGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACTGCCGTCATCCCCACTTGGCAGGCAT; TGACCACGCTTGACCTGAGCCACAACAGCATCTCCGAGATCGACGAGTCTGTGAAACTGA; TCCCAAAGATTGAGTTCCTGGACCTGAGTCACAATGGATTGCTGGTTGTGGACAATCTGC; AGCACCTGTATAACCTTGTGCATCTGGACCTGTCCTACAACAAGCTCTCCTCCTTGGAAG; GGCTTCACACCAAGCTGGGGAACATCAAGACCTTAAACCTGGCAGGCAACCTCCTAGAGA; GTCTGAGTGGCCTGCACAAGCTCTACTCACTGGTCAACCTGGATCTCCGGGACAACAGGA; TCGAACAGATGGAGGAGGTCCGGAGCATAGGCAGCCTCCCGTGTCTGGAGCACGTGTCTC; TGCTGAACAACCCTCTGAGCATCATCCCCGACTACCGGACCAAGGTGCTGGCTCAGTTCG; GAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACAGTGACCACAGAGAAGGAGCTGGACA; CTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAGGAGGTCAAGTCCAAACTGAGCAACC; CAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCAGCTGCCCCCTGCATCAGACCCAGCA; GCTCCCCTCCCACTGTGGCTCCCGCATCTGCCTCCCTGCCCCAGCCCATCCTCTCTAACC; AAGGAATCATGTTCGTTCAGGAGGAGGCCCTGGCCAGCAGCCTCTCGTCCACTGACAGTC; TGACTCCCGAGCACCAGCCCATTGCCCAGGGATGTTCTGATTCCTTGGAGTCCATCCCTG; CGGGACAGGCAGCTTCCGATGATTTAAGGGACGTGCCAGGAGCTGTTGGTGGTGCAAGCC; CAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCGGGGTCTGGCCAGATCATCTTCCTGC; CCTTCACCTGCATTGGCTACACGGCCACCAATCAGGACTTCATCCAGCGCCTGAGCACAC; TGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCCTGGATCGAGGCTGCCAACCAGCGGG; AGGAGGGCCAGGGTGAACAGGGCGAGGAGGAGGATGAGGAGGAGGAAGAAGAGGAGGACG; TGGCTGAGAACCGCTACTTTGAAATGGGGCCCCCAGACGTGGAGGAGGAGGAGGGAGGAG; GCCAGGGGGAGGAAGAGGAGGAGGAAGAGGAGGATGAAGAGGCCGAGGAGGAGCGCCTGG; CTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTCCTGCTGGAGCACATCCGCATCCTCA; AGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGCAGTATCCGCCAGTTCGCCGCCTGCC; TTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAGATCCCGCACCAGGAGTCTCGGGGCA; GCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTCTTTTGCTTCCCGCATGGCGACCTCA; CCGAGTTTGGCTTCCTCATGCCGGAGCTGTGTCTGGTGCTCAAGGTACGGCACAGTGAGA; ACACGCTCTTCATTATCTCGGACGCCGCCAACCTGCACGAGTTCCACGCGGACCTGCGCT; CATGCTTTGCACCCCAGCACATGGCCATGCTGTGTAGCCCCATCCTCTACGGCAGCCACA; CCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACCTTCTACAAGGTGGCTGGCGGCTGCC; AGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTGGTCTACAGTGACAAGCGCATGGTGC; AGACGGCCGCCGGGGACTACTCAGGCAACATCGAGTGGGCCAGCTGCACACTCTGTTCAG; CCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCCGTCAAGTCCGCCGCCATCCCCTACT; GGCTGTTGCTCACGCCCCAGCACCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCA; ACCGTGGCACCCACAACTGTCGCAACCGCAACAGCTTCAAGCTCAGCCGTGTGCCGCTCT; CCACCGTGCTGCTGGACCCCACACGCAGCTGTACCCAGCCTCGGGGCGCCTTTGCTGATG; GCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTTGTCACTGCCATCTTCGTGCTGCCCC; ACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAGCTGCGGGCCTCGCTGCAGGACCTGA; AGACTGTGGTCATCGCCAAGACCCCCGGGACGGGAGGCAGCCCCCAGGGCTCCTTTGCGG; ATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGACCAGCGTCCCCAGGAGGTCCCAGCAG; AGGCTCTGGCCCCGGCCCCAGTGGAAGTCCCAGCTCCAGCCCCTGCAGCAGCCTCAGCCT; CAGGCCCAGCGAAGACTCCGGCCCCAGCAGAGGCCTCAACTTCAGCTTTGGTCCCAGAGG; AGACGCCAGTGGAAGCTCCAGCCCCACCCCCAGCCGAGGCCCCTGCCCAGTACCCGAGTG; AGCACCTCATCCAGGCCACCTCGGAGGAGAATCAGATCCCCTCGCACTTGCCTGCCTGCC; CGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGCGCCATCATCGAGCTCTTCCACAGCA; GCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCACCTCATGTGGTCCTCGGTGGTGTTCT; ACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTGCTGCTCTCCACCAAGGCTGTGTACT; TTGTGCTCCACGACGGCCTCCGCCGCTACTTCTCAGAGCCACTGCAGGATTTCTGGCATC; AGAAAAACACCGACTACAACAACAGCCCTTTCCACATCTCCCAGTGCTTCGTGCTAAAGC; TTAGTGACCTGCAGTCAGTCAATGTGGGGCTTTTCGACCAGCATTTCCGGCTGACGGGTT; CCACCCCGATGCAGGTGGTCACGTGCTTGACGCGGGACAGCTACCTGACGCACTGCTTCC; TCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAACGCACGCCCTCGCCGGAGCCTGTTG; ACAAGGACTTCTACTCCGAGTTTGGGAACAAGACCACAGGGAAGATGGAGAACTACGAGC; TGATCCACTCTAGTCGCGTCAAGTTTACCTACCCCAGTGAGGAGGAGATTGGGGACCTGA; CGTTCACTGTGGCCCAAAAGATGGCTGAGCCAGAGAAGGCCCCAGCCCTCAGCATCCTGC; TGTACGTGCAGGCCTTCCAGGTGGGCATGCCACCCCCTGGGTGCTGCAGGGGCCCCCTGC; GCCCCAAGACACTCCTGCTCACCAGCTCCGAGATCTTCCTCCTGGATGAGGACTGTGTCC; ACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCGCAGAGAGACAGGTACCGGCTGGACG; ATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTCATGGGCTACCAGACCTACCCGCAGG; CCCTCACCCTCGTCTTCGATGACGTGCAAGGTCATGACCTCATGGGCAGTGTCACCCTGG; ACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCGTGAAGTCCAGT; GGCAGGTGTTTGTCCCCAGTGCTGAGAGCAGAGAGAAGCTCATCTCGCTGTTGGCTCGCC; AGTGGGAGGCCCTGTGTGGCCGTGAGCTGCCTGTCGAGCTCACCGGCTAGCCCAGGCCAC; AGCCAGCCTGTCGTGTCCAGCCTGACGCCTACTGGGGCAGGGCAGCAGGCTTTTGTGTTC; TCTAAAAATGTTTTATCCTCCCTTTGGTACCTTAATTTGACTGTCCTCGCAGAGAATGTG; AACATGTGTGTGTGTTGTGTTAATTCTTTCTCATGTTGGGAGTGAGAATGCCGGGCCCCT; CAGGGCTGTCGGTGTGCTGTCAGCCTCCCACAGGTGGTACAGCCGTGCACACCAGTGTCG; TGTCTGCTGTTGTGGGACCGTTGTTAACACGTGACACTGTGGGTCTGACTTTCTCTTCTA; CACGTCCTTTCCTGAAGTGTCGAGTCCAGTCCTTTGTTGCTGTTGCTGTTGCTGTTGCTG; TTGCTGTTGGCATCTTGCTGCTAATCCTGAGGCTGGTAGCAGAATGCACATTGGAAGCTC; CCACCCCATATTGTTCTTCAAAGTGGAGGTCTCCCCTGATCCAGACAAGTGGGAGAGCCC; GTGGGGGCAGGGGACCTGGAGCTGCCAGCACCAAGCGTGATTCCTGCTGCCTGTATTTTT; TATTCCAATAAAGCAGAGTTTGACACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007184 unedited; AATTTTCCCGCCCGTTGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG; CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC; GAATTCGGCACGAGGGTGGCGGCGGAGACCCGAACATGGCGACCGCGCGCACCTTCGGGC; CCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCTCGGAGCTTGTGGACA; CTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAAGCACC; GCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAA; ACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGG; AGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCA; GAGTACTGGCCCACTTCTTGCATTTTCACTTCTATGAGATAAATGGCATCACCGCGGCAC; TGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTCCTGGGGGCCGGCGAGGTCTTTGCCA; TTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGGGAAAGCCCACGTGCG; CCAGTGNGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCTTTAGT; ACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGNGACCAGCACATTCAGGAGCAGCTCC; TGCCGTTCGACCTATCATATTCAAGTCCCTGCATCAGGTGGNAGATAGTCACTGTGATGC; TAAGCACATCAGAGGGCTGGTCGCATCGAAGCCACCTTAGCACGCTGAGTGTCCCGCTCT; CAGCACCTCGATGAGGAAGTCCNTGNTNNCTGAGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007184 unedited; NGGGGGGTACCACTATGNNACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTCGGGGTCAAACTCTGCTTTATTGGAATAAAAAATACAG; GCAGCAGGAATCACGCTTGGTGCTGGCAGCTCCAGGTCCCCTGCCCCCACGGGCTCTCCC; ACTTGTCTGGATCAGGGGAAACCTCCACTTTGAAAAACAATATGGGGTGGGAGCTTCCAA; TGTGCATTCTGCTACCAGCCTCAGGATTAGCAGCAAGATGCCAACAGCAACAGCAACAGC; AACAGCAACAGCAACAAAGGACTGGACTCGACACTTCAGGAAAGGACGTGTAAAAAAAAA; AGTCAGACCCCCAGTGTCACGTGTTAACAACGGTCCCACAACAGCAAACACGAAACTGGT; GTGCACGGCTGTACCACCTGGGGGAGGCTGAAAGCACACCGACAGCCCTGAGGGGCCCGG; CATTCTTACTCCCCACATGAGAAAGAATTAACACAACCCCCCCCCCTGTTCACATTTTCT; GCGAGGACAGTCAAATTAAGGTACCCAAGGGGGGATAAAACATTTTTAGAGAAAAAAAAA; ACCTGGTGCCCTGCCCCGAAGGCCTTAGGCTGGACACCAAAGGCTGGCTTTGGCCCGGGC; TAACCCGGGAAACCTGAAAGGGAGTTCACGGGCCCCCCGGGGCCCCCCCCTGGGGAAGCC; CACCAGGAGAAAGAAGCTTTTCTTTGGCTCTTAAAACCTGGGGACAAAAACCTTGCCACT; GGGGATTAAACGGCCCTGGGGGTGCTTTTACCCGGGGCACCATGGGCCTCTCCCCAAAAG; GGGCCAGGTGAAAACTCGCCCCGAAAGACAATAACCTTCCCTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007184
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007184.2, NP_009115.2
• RefSeq Size: 5139 bp
• RefSeq ORF: 4515 bp
• Locus ID: 11188
• UniProt ID: Q9Y2I1
• Cytogenetics: 3p21.1
• Domains: LRR, LRR_SD22, PX
• Protein Families: Druggable Genome
• Summary: This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as IRAS-M).