RIP (RIPK1) (NM_003804) Human Untagged Clone

SKU SC108466

RIPK1 (untagged)-Human receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RIP
• Synonyms: AIEFL; IMD57; RIP; RIP-1; RIP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003804 edited; ATGCAACCAGACATGTCCTTGAATGTCATTAAGATGAAATCCAGTGACTTCCTGGAGAGT; GCAGAACTGGACAGCGGAGGCTTCGGGAAGGTGTCTCTGTGTTTCCACAGAACCCAGGGA; CTCATGATCATGAAAACAGTGTACAAGGGGCCCAACTGCATTGAGCACAACGAGGCCCTC; TTGGAGGAGGCGAAGATGATGAACAGACTGAGACACAGCCGGGTGGTGAAGCTCCTGGGC; GTCATCATAGAGGAAGGGAAGTACTCCCTGGTGATGGAGTACATGGAGAAGGGCAACCTG; ATGCACGTGCTGAAAGCCGAGATGAGTACTCCGCTTTCTGTAAAAGGAAGGATAATTTTG; GAAATCATTGAAGGAATGTGCTACTTACATGGAAAAGGCGTGATACACAAGGACCTGAAG; CCTGAAAATATCCTTGTTGATAATGACTTCCACATTAAGATCGCAGACCTCGGCCTTGCC; TCCTTTAAGATGTGGAGCAAACTGAATAATGAAGAGCACAATGAGCTGAGGGAAGTGGAC; GGCACCGCTAAGAAGAATGGCGGCACCCTCTACTACATGGCGCCCGAGCACCTGAATGAC; GTCAACGCAAAGCCCACAGAGAAGTCGGATGTGTACAGCTTTGCTGTAGTACTCTGGGCG; ATATTTGCAAATAAGGAGCCATATGAAAATGCTATCTGTGAGCAGCAGTTGATAATGTGC; ATAAAATCTGGGAACAGGCCAGATGTGGATGACATCACTGAGTACTGCCCAAGAGAAATT; ATCAGTCTCATGAAGCTCTGCTGGGAAGCGAATCCGGAAGCTCGGCCGACATTTCCTGGC; ATTGAAGAAAAATTTAGGCCTTTTTATTTAAGTCAATTAGAAGAAAGTGTAGAAGAGGAC; GTGAAGAGTTTAAAGAAAGAGTATTCAAACGAAAATGCAGTTGTGAAGAGAATGCAGTCT; CTTCAACTTGATTGTGTGGCAGTACCTTCAAGCCGGTCAAATTCAGCCACAGAACAGCCT; GGTTCACTGCACAGTTCCCAGGGACTTGGGATGGGTCCTGTGGAGGAGTCCTGGTTTGCT; CCTTCCCTGGAGCACCCACAAGAAGAGAATGAGCCCAGCCTGCAGAGTAAACTCCAAGAC; GAAGCCAACTACCATCTTTATGGCAGCCGCATGGACAGGCAGACGAAACAGCAGCCCAGA; CAGAATGTGGCTTACAACAGAGAGGAGGAAAGGAGACGCAGGGTCTCCCATGACCCTTTT; GCACAGCAAAGACCTTACGAGAATTTTCAGAATACAGAGGGAAAAGGCACTGCTTATTCC; AGTGCAGCCAGTCATGGTAATGCAGTGCACCAGCCCTCAGGGCTCACCAGCCAACCTCAA; GTACTGTATCAGAACAATGGATTATATAGCTCACATGGCTTTGGAACAAGACCACTGGAT; CCAGGAACAGCAGGTCCCAGAGTTTGGTACAGGCCAATTCCAAGTCATATGCCTAGTCTG; CATAATATCCCAGTGCCTGAGACCAACTATCTAGGAAATACACCCACCATGCCATTCAGC; TCCTTGCCACCAACAGATGAATCTATAAAATATACCATATACAATAGTACTGGCATTCAG; ATTGGAGCCTACAATTATATGGAGATTGGTGGGACGAGTTCATCACTACTAGACAGCACA; AATACGAACTTCAAAGAAGAGCCAGCTGCTAAGTACCAAGCTATCTTTGATAATACCACT; AGTCTGACGGATAAACACCTGGACCCAATCAGGGAAAATCTGGGAAAGCACTGGAAAAAC; TGTGCCCGTAAACTGGGCTTCACACAGTCTCAGATTGATGAAATTGACCATGACTATGAG; CGAGATGGACTGAAAGAAAAGGTTTACCAGATGCTCCAAAAGTGGGTGATGAGGGAAGGC; ATAAAGGGAGCCACGGTGGGGAAGCTGGCCCAGGCGCTCCACCAGTGTTCCAGGATCGAC; CTTCTGAGCAGCTTGATTTACGTCAGCCAGAACTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003804 unedited; CAGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGTTCTT; GAGCTTCAGAATGCAACCAGACTGTCCTTGAATGTCATTAAGATGAAATCCAGTGACTTC; CTGGAGAGTGCAGAACTGGACAGCGGAGGCTTCGGGAAGGTGTCTCTGTGTTTCCACAGA; ACCCAGGGACTCATGATCATGAAAACAGTGTACAAGGGGCCCAACTGCATTGAGCACAAC; GAGGCCCTCTTGGAGGAGGCGAAGATGATGAACAGACTGAGACACAGCCGGGTGGTGAAG; CTCCTGGGCGTCATCATAGAGGAAGGGAAGTACTCCCTGGTGATGGAGTACATGGAGAAG; GGCAACCTGATGCACGTGCTGAAAGCCGAGATGAGTACTCCGCTTTCTGTAAAAGGAAGG; ATAATTTTGGAAATCATTGAAGGAATGTGCTACTTACATGGAAAAGGCGTGATACACAAG; GACCTGAAGCCTGAAAATATCCTTGTTGATAATGACTTCCACATTAAGATCGCAGACCTC; GGCCTTGCCTCCTTTAAGATGTGGAGCAAACTGAATAATGAAGAGCACAATGAGCTGAGG; GAAGTGGACGGCACCGCTAAGAAGAATGGCGGCACCCTCTACTACATGGCGCCCGAGCAC; CTGAATGACGTCAACGCAAAGCCCACAGAGAAGTCGGATGTGTACAGCTTTGCTGTAGTA; CTCTGGGCGATATTTGCAAATAAGGAGCCCATATGAAATGCTATCTGTGAGCAGCAGTTG; ATAATGTGCATAAAATCTGGGAACAGGCCAGATGTGGATGACATCACTGAGTACTGCCCA; AGAGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003804 unedited; TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTGGGGGATAAATTTTTATTTTAAAAA; GTGTTAAAAAATGCCTTGCCCTCTTGCCTTTGAACATTAATTATTCAACCTCTTTGGAAT; CTAAGAGATTTGATAACACCATTTGGATGGTGGTACAAGGAGCAAACAGAACTCACCCAG; GCCCCTCCAGCAAAGCCTGGAGCCTCCCTCCATTCTCCCTTTTGGGCTGTAGGAGCCAAG; GCTGCGTGGTGGCCAAGTAGTAACCGGACAGGTGCAGACAGGAGAGCCGAGGCTCATTAC; TTTACAGCAGTGCTCTTCTCAACCGGGGCAATTTTCCCCTTTCCTTGGACCATTTGACAA; TGTTTAGAGACTATTGTCACAACTGGGGCGGGGGGCTGGGGAGAACAGTGCCCCTGGCAT; CTAGTAGGTGGGGGCAGGGATGCTACTAAACATCCTACAAGGAACAGGACAGTCCCCACA; ACAGAATTACCCCAGCCCGAGATGCCGACAGTGGCAAGGCTGAGACAGCCTGCTCTGCAC; CCCATTTCAGGGGTGTGTATGTGTCTGCTAGAGTTGAGGGGATTTACAATCAGAATCAAC; CACACTGTTATCAGCCCAAGAACCAGAATCTGTCCGACAGCCAGATTGCCATTACACACC; CTTTTTGGCCTCCCTCTGGGGAGAGCTAGCACAAAGCCTTCCTTTGTCCGTTTACAAAAT; GCCAGTGACACTGAGCGGGCTACCCAGCTTAAGCTTTTATATTAGTCTTTAATCCCCCCC; AAGTTTAAGTGGCCTTTGAAGGCTGGTGCTAAAGCCCAATACCCACTTAGAGCTGCCTTT; TTACTTTGTCCCCAGAAAAATTGAAAATCTTTCAGTCCCCGATTAACCGAGGAACCCTTA; ACAGTTAACCCTTGGGTTCTGGCTACCCAAGTAAAAAAAAATTTCTGTTCTTCCAAGCCC; TACGCTGGGAGGCCAAATGCCGAAAACTTTAAGTATCAGATTTTGTGACCACGTAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003804
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003804.3, NP_003795.2
• RefSeq Size: 3864 bp
• RefSeq ORF: 2016 bp
• Locus ID: 8737
• UniProt ID: Q13546
• Cytogenetics: 6p25.2
• Domains: DEATH, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Summary: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).