NDRG4 (NM_020465) Human Untagged Clone

CAT#: SC108447

NDRG4 (untagged)-Human NDRG family member 4 (NDRG4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDRG4
• Synonyms: BDM1; SMAP-8; SMAP8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108447 sequence for NM_020465 edited (data generated by NextGen Sequencing)
  ATGGCCGGGCTGCAGGAGCTGCGATTCCCTGAGGAGAAGCCGCTGCTCCGGGGCCAGGAC
  GCCACCGAGCTGGAGAGCTCCGATGCCTTCCTCTTGGCTGCAGACACAGACTGGAAGGAA
  CATGACATCGAGACACCCTACGGCCTTCTGCATGTAGTGATCCGGGGCTCCCCCAAGGGG
  AACCGCCCAGCCATCCTCACCTACCATGATGTGGGCCTCAACCACAAACTATGCTTCAAC
  ACCTTCTTCAACTTCGAGGACATGCAGGAGATCACCAAGCACTTTGTGGTGTGTCACGTG
  GATGCCCCTGGACAACAGGTGGGGGCGTCGCAGTTTCCTCAGGGGTACCAGTTCCCCTCC
  ATGGAGCAGCTGGCTGCCATGCTCCCCAGCGTGGTGCAGCATTTCGGGTTCAAGTATGTG
  ATTGGCATCGGAGTGGGCGCCGGAGCCTATGTGCTGGCCAAGTTTGCACTCATCTTCCCC
  GACCTGGTGGAGGGGCTGGTGCTGGTGAACATCGACCCCAATGGCAAAGGCTGGATAGAC
  TGGGCTGCCACCAAGCTCTCCGGCCTAACTAGCACTTTACCCGACACGGTGCTCTCCCAC
  CTCTTCAGCCAGGAGGAGCTGGTGAACAACACAGAGTTGGTGCAGAGCTACCGGCAGCAG
  ATTGGGAACGTGGTGAACCAGGCCAACCTGCAGCTCTTCTGGAACATGTACAACAGCCGC
  AGAGACCTGGACATTAACCGGCCTGGAACGGTGCCCAATGCCAAGACGCTCCGCTGCCCC
  GTGATGCTGGTGGTTGGGGATAATGCACCCGCTGAGGACGGGGTGGTGGAGTGCAACTCC
  AAACTGGACCCGACCACTACGACCTTCCTGAAGATGGCAGACTCTGGAGGGCTGCCCCAG
  GTCACACAGCCAGGGAAGCTGACTGAAGCCTTCAAATACTTCCTGCAAGGCATGGGCTAC
  ATGCCCTCAGCCAGCATGACCCGCCTGGCACGCTCCCGCACTGCATCCCTCACCAGTGCC
  AGCTCGGTGGATGGCAGCCGCCCACAGGCCTGCACCCACTCGGAGAGCAGCGAGGGGCTG
  GGCCAGGTCAACCACACCATGGAGGTGTCCTGTTGA
  
  Clone variation with respect to NM_020465.3
  1062 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020465 unedited
  GCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCCCGCACCTGCAC
  CCGCGCGTCGGCGGCGCCGAAGCCCCGCTCCCCGCCTGCGCGTCTGTCTCGTCCGCATCT
  CCGCGGCCTCCTGCTCCACGACGTGACCATGGCCGGGCTGCAGGAGCTGCGATTCCCTGA
  GGAGAAGCCGCTGCTCCGGGGCCAGGACGCCACCGAGCTGGAGAGCTCCGATGCCTTCCT
  CTTGGCTGCAGACACAGACTGGAAGGAACATGACATCGAGACACCCTACGGCCTTCTGCA
  TGTAGTGATCCGGGGCTCCCCCAAGGGGAACCGCCCAGCCATCCTCACCTACCATGATGT
  GGGCCTCAACCACAAACTATGCTTCAACACCTTCTTCAACTTCGAGGACATGCAGGAGAT
  CACCAAGCACTTTGTGGTGTGTCACGTGGATGCCCCTGGACAACAGGTGGGGGCGTCGCA
  GTTTCCTCAGGGGTACCAGTTCCCCTCCATGGAGCAGCTGGCTGCCATGCTCCCCAGCGT
  GGTGCAGCATTTCGGGTTCAAGTATGTGATTGGCATCGGAGTGGGCGCCGGAGCCTATGT
  GCTGGCCAAGTTTGCACTCATCTTCCCCGACCTGGTGGAGGGGCTGGTGCTGGTGAACAT
  CGACCCCAATGGCAAAGGCTGGATAGACTGGGCTGCCACCAAGCTCTCCGGCCTAACTAG
  CACTTTACCCGACACGGTGCTCTCCCACCTCTTCAGCCCAGAGGAGCTGGTGAACACACA
  GAGNTGGTGCAGAGCTACCGGCAGCAGATTGNGAACGTGGGTGACCANNGCCACCTGCAG
  CTCTTCTGGACATGTACACAGCCGCAGAACCTGGACATTACCCGGCTGGNNACGTGCCCA
  TGCCAGACGCTCGCTGCCCCGGATGCTGTGGNTGGGATATGCACCCTGAGGACGGGGGTG
  GAGGCACTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020465 unedited
  CTACTCTGNAACGACGGCCGCTATTCTAGNGATCAGNCCCTTTTTTTTTTTTTTTTTTTT
  TTCTTTTCTTTTCCTTCCTAAGGGCCCTTTATTAGTTTTTCAGCACAGTTAACTCTTTCT
  ACGTTGACTAAAGTTTTGTTTTACATTTTATCATCTCATGACAGCAGCCACCAAAATACA
  CAAAATTAGGCTCTTTACTTTGGGCGGGAAAAAAAAATAATAAACATTAAAAGTTCACCT
  TGACCAAAGAAACAACCTCCAAAAAAACACCACACATGGGAGGACCCGATGTACACACAC
  ACACACACACACATATAAGGTATAGATATTTATAGATACATCTGGAAACAGCAAAAAACA
  AAATTCCAAGATTTGCAGGGTTATCTACAGTAAAAGGGATGAATTCTACCAGCTTTCAAA
  CAGTCACATTCAGTATTCAAAAAAAGCACATGAGGGGGTAAAACTGGGCAAAAACTAACA
  CGCCACCAGGCTAGTCATGGCTTAAAATTATAAAAGGGGGGGGATGGGTGTAGAGGAAAT
  ACCCATATAGGGGTCCTCTCAGCCTAAAAACACCCGCCACAACCCTTCCCCCGTCCACAG
  CCCTGCATGTTTTCACTGCTTTCTCCCGTTTGGGGGTTTGGGATTATTGATTAAGGGCCT
  GAAACAAATGGCTTTGGTTTCTGGGCCCTAAGATGCTATGGGGAAAGGGGAGCGGATGGA
  AAACATACAACCCATTCCTGGGCTTAAGGGGACACAAAGAGCTGGGGTGAAGGGGAAACC
  TTCTCTTTACCCGACCCTGTTATGTCCAGACCTTAACCCCCATTTAGCCCATCCAGGCGT
  TATGGGGTGGGGACCGCGAAACCCTGCTGCCTTGGGCCATAGACTAATAACCTCTTTTTT
  CTTATGGGGCGCCGGGACAACCTGGACATCCGGACAAAGCTTGCCTAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020465
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020465.2, NP_065198.1
• RefSeq Size: 3288 bp
• RefSeq ORF: 1116 bp
• Locus ID: 65009
• UniProt ID: Q9ULP0
• Cytogenetics: 16q21
• Domains: Ndr
• Gene Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) differs in the 5' UTR which results in the use of an in-frame downstream translation initiation codon, compared to variant 2. The encoded protein (isoform 1; also known as NDRG4-H) has a shorter N-terminus, compared to isoform 2. Variants 1 and 3 encode the same isoform.