NDRG4 (NM_020465) Human Untagged Clone

SKU SC108447

NDRG4 (untagged)-Human NDRG family member 4 (NDRG4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDRG4
• Synonyms: BDM1; SMAP-8; SMAP8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108447 sequence for NM_020465 edited (data generated by NextGen Sequencing); ATGGCCGGGCTGCAGGAGCTGCGATTCCCTGAGGAGAAGCCGCTGCTCCGGGGCCAGGAC; GCCACCGAGCTGGAGAGCTCCGATGCCTTCCTCTTGGCTGCAGACACAGACTGGAAGGAA; CATGACATCGAGACACCCTACGGCCTTCTGCATGTAGTGATCCGGGGCTCCCCCAAGGGG; AACCGCCCAGCCATCCTCACCTACCATGATGTGGGCCTCAACCACAAACTATGCTTCAAC; ACCTTCTTCAACTTCGAGGACATGCAGGAGATCACCAAGCACTTTGTGGTGTGTCACGTG; GATGCCCCTGGACAACAGGTGGGGGCGTCGCAGTTTCCTCAGGGGTACCAGTTCCCCTCC; ATGGAGCAGCTGGCTGCCATGCTCCCCAGCGTGGTGCAGCATTTCGGGTTCAAGTATGTG; ATTGGCATCGGAGTGGGCGCCGGAGCCTATGTGCTGGCCAAGTTTGCACTCATCTTCCCC; GACCTGGTGGAGGGGCTGGTGCTGGTGAACATCGACCCCAATGGCAAAGGCTGGATAGAC; TGGGCTGCCACCAAGCTCTCCGGCCTAACTAGCACTTTACCCGACACGGTGCTCTCCCAC; CTCTTCAGCCAGGAGGAGCTGGTGAACAACACAGAGTTGGTGCAGAGCTACCGGCAGCAG; ATTGGGAACGTGGTGAACCAGGCCAACCTGCAGCTCTTCTGGAACATGTACAACAGCCGC; AGAGACCTGGACATTAACCGGCCTGGAACGGTGCCCAATGCCAAGACGCTCCGCTGCCCC; GTGATGCTGGTGGTTGGGGATAATGCACCCGCTGAGGACGGGGTGGTGGAGTGCAACTCC; AAACTGGACCCGACCACTACGACCTTCCTGAAGATGGCAGACTCTGGAGGGCTGCCCCAG; GTCACACAGCCAGGGAAGCTGACTGAAGCCTTCAAATACTTCCTGCAAGGCATGGGCTAC; ATGCCCTCAGCCAGCATGACCCGCCTGGCACGCTCCCGCACTGCATCCCTCACCAGTGCC; AGCTCGGTGGATGGCAGCCGCCCACAGGCCTGCACCCACTCGGAGAGCAGCGAGGGGCTG; GGCCAGGTCAACCACACCATGGAGGTGTCCTGTTGA; ; Clone variation with respect to NM_020465.3; 1062 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020465 unedited; GCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCCCGCACCTGCAC; CCGCGCGTCGGCGGCGCCGAAGCCCCGCTCCCCGCCTGCGCGTCTGTCTCGTCCGCATCT; CCGCGGCCTCCTGCTCCACGACGTGACCATGGCCGGGCTGCAGGAGCTGCGATTCCCTGA; GGAGAAGCCGCTGCTCCGGGGCCAGGACGCCACCGAGCTGGAGAGCTCCGATGCCTTCCT; CTTGGCTGCAGACACAGACTGGAAGGAACATGACATCGAGACACCCTACGGCCTTCTGCA; TGTAGTGATCCGGGGCTCCCCCAAGGGGAACCGCCCAGCCATCCTCACCTACCATGATGT; GGGCCTCAACCACAAACTATGCTTCAACACCTTCTTCAACTTCGAGGACATGCAGGAGAT; CACCAAGCACTTTGTGGTGTGTCACGTGGATGCCCCTGGACAACAGGTGGGGGCGTCGCA; GTTTCCTCAGGGGTACCAGTTCCCCTCCATGGAGCAGCTGGCTGCCATGCTCCCCAGCGT; GGTGCAGCATTTCGGGTTCAAGTATGTGATTGGCATCGGAGTGGGCGCCGGAGCCTATGT; GCTGGCCAAGTTTGCACTCATCTTCCCCGACCTGGTGGAGGGGCTGGTGCTGGTGAACAT; CGACCCCAATGGCAAAGGCTGGATAGACTGGGCTGCCACCAAGCTCTCCGGCCTAACTAG; CACTTTACCCGACACGGTGCTCTCCCACCTCTTCAGCCCAGAGGAGCTGGTGAACACACA; GAGNTGGTGCAGAGCTACCGGCAGCAGATTGNGAACGTGGGTGACCANNGCCACCTGCAG; CTCTTCTGGACATGTACACAGCCGCAGAACCTGGACATTACCCGGCTGGNNACGTGCCCA; TGCCAGACGCTCGCTGCCCCGGATGCTGTGGNTGGGATATGCACCCTGAGGACGGGGGTG; GAGGCACTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020465 unedited; CTACTCTGNAACGACGGCCGCTATTCTAGNGATCAGNCCCTTTTTTTTTTTTTTTTTTTT; TTCTTTTCTTTTCCTTCCTAAGGGCCCTTTATTAGTTTTTCAGCACAGTTAACTCTTTCT; ACGTTGACTAAAGTTTTGTTTTACATTTTATCATCTCATGACAGCAGCCACCAAAATACA; CAAAATTAGGCTCTTTACTTTGGGCGGGAAAAAAAAATAATAAACATTAAAAGTTCACCT; TGACCAAAGAAACAACCTCCAAAAAAACACCACACATGGGAGGACCCGATGTACACACAC; ACACACACACACATATAAGGTATAGATATTTATAGATACATCTGGAAACAGCAAAAAACA; AAATTCCAAGATTTGCAGGGTTATCTACAGTAAAAGGGATGAATTCTACCAGCTTTCAAA; CAGTCACATTCAGTATTCAAAAAAAGCACATGAGGGGGTAAAACTGGGCAAAAACTAACA; CGCCACCAGGCTAGTCATGGCTTAAAATTATAAAAGGGGGGGGATGGGTGTAGAGGAAAT; ACCCATATAGGGGTCCTCTCAGCCTAAAAACACCCGCCACAACCCTTCCCCCGTCCACAG; CCCTGCATGTTTTCACTGCTTTCTCCCGTTTGGGGGTTTGGGATTATTGATTAAGGGCCT; GAAACAAATGGCTTTGGTTTCTGGGCCCTAAGATGCTATGGGGAAAGGGGAGCGGATGGA; AAACATACAACCCATTCCTGGGCTTAAGGGGACACAAAGAGCTGGGGTGAAGGGGAAACC; TTCTCTTTACCCGACCCTGTTATGTCCAGACCTTAACCCCCATTTAGCCCATCCAGGCGT; TATGGGGTGGGGACCGCGAAACCCTGCTGCCTTGGGCCATAGACTAATAACCTCTTTTTT; CTTATGGGGCGCCGGGACAACCTGGACATCCGGACAAAGCTTGCCTAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_020465
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020465.2, NP_065198.1
• RefSeq Size: 3288 bp
• RefSeq ORF: 1116 bp
• Locus ID: 65009
• UniProt ID: Q9ULP0
• Cytogenetics: 16q21
• Domains: Ndr
• Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) differs in the 5' UTR which results in the use of an in-frame downstream translation initiation codon, compared to variant 2. The encoded protein (isoform 1; also known as NDRG4-H) has a shorter N-terminus, compared to isoform 2. Variants 1 and 3 encode the same isoform.