Adrenodoxin (FDX1) (NM_004109) Human Untagged Clone

CAT#: SC108419

FDX1 (untagged)-Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Adrenodoxin
• Synonyms: ADX; FDX; LOH11CR1D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108419 sequence for NM_004109 edited (data generated by NextGen Sequencing)
  ATGGCTGCCGCTGGGGGCGCCCGGCTGCTGCGCGCCGCTTCTGCTGTCCTCGGCGGCCCG
  GCCGGCCGGTGGCTGCACCACGCTGGGTCCCGCGCTGGATCCAGCGGCCTGCTGAGGAAC
  CGGGGGCCGGGCGGGAGCGCGGAGGCGAGCCGGTCGCTGAGCGTGTCGGCGCGGGCCCGG
  AGCAGCTCAGAAGATAAAATAACAGTCCACTTTATAAACCGTGATGGTGAAACATTAACA
  ACCAAAGGAAAAGTTGGTGATTCTCTGCTAGATGTTGTGGTTGAAAATAATCTAGATATT
  GATGGCTTTGGTGCATGTGAGGGAACCCTGGCTTGTTCAACCTGTCACCTCATCTTTGAA
  GATCACATATATGAGAAGTTAGATGCAATCACTGATGAGGAGAATGACATGCTCGATCTG
  GCATATGGACTAACAGACAGATCACGGTTGGGCTGCCAAATCTGTTTGACAAAATCTATG
  GACAATATGACTGTTCGAGTGCCTGAAACAGTGGCTGATGCCAGACAATCCATTGATGTG
  GGCAAGACCTCCTGA
  
  Clone variation with respect to NM_004109.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004109 unedited
  GCACGAGGGGTGCTTCCAGCAGGTCTCTCCGCCACTCCAGCCCCGCGCCCCTCGCCGCGG
  CCCTCGGGCGTCTGCGCCGCAGCTGCCGCCCCCGCCTCTTTGGAGTCTCTCGCGGCCTCA
  AAGCGCGGCCTGCGTCGCTTCCGGCAGTTCCCGACCGCGGGCGATGGCTGCCGCTGGGGG
  CGCCCGGCTGCTGCGCGCCGCTTCTGCTGTCCTCGGCGGCCCGGCCGGCCGGTGGCTGCA
  CCACGCTGGGTCCCGCGCTGGATCCAGCGGCCTGCTGAGGAACCGGGGGCCGGGCGGGAG
  CGCGGAGGCGAGCCGGTCGCTGAGCGTGTCGGCGCGGGCCCGGAGCAGCTCAGAAGATAA
  AATAACAGTCCACTTTATAAACCGTGATGGTGAAACATTAACAACCAAAGGAAAAGTTGG
  TGATTCTCTGCTAGATGTTGTGGTTGAAAATAATCTAGATATTGATGGCTTTGGTGCATG
  TGAGGGAACCCTGGCTTGTTCAACCTGTCACCTCATCTTTGAAGATCACATATATGAGAA
  GTTAGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004109 unedited
  GCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACTTTTATGAATTTATTTAT
  AGTAAAGATAAGAGTACGAATGTCTTCCACACATACGTGGTAATCTGTGGTGCTTGCCAC
  TCCCTCACTCCTGGGACTGACACACTGCAGCCCACGCTGCTCACCCTGCAGCCCCATCCT
  TGCGTGAGAGAATGGCGCCACCTGGGGCTGTGCAACGTGTCAGCCTGCTCATTCCCTTCT
  CCAGCTCAGGACAGAGAAACACAATTCATTCATAAGGTTTCCATTCTGTTATAGGCAGAG
  ATTTATAAATCAGCATTATTTTTCAAAGAGTACAAAGTTAAACCATTCTGCTTAGAAAAG
  AATAAACACATCCAATCATTTTTCCTCTAGTTTAGTCAAAACAGAGATCAAACTTCTTTG
  ATAGCATTATAATGTCTACGTCCAGACTTAGTCTTCCGAATCAAAATTTTGAAATATTTA
  TTTATAAGGTCAATAACTATCTTTGAAGTTAAGCAGTTTACTGAGCAATTAAATATTTTT
  GTCCATACACATTCAGATTTAACTACTACTATAAATAAAATAGTCATAATTTCTCCAGTT
  TCAGTCTGAACACATAGCTTATAGGAATAAGACACAGGTTCTAGCACAGATTTTCTAATT
  AGTGGTATGGCCCTAAACAAGCCAGGTTAGATCTCTGCCTTCACCCCATCAAACAGGAAA
  TATANTATATTTCCTTAACCTATCTCCTAGGTAACAGATGAGATTATATGAAACATTTTG
  CTAAAGTCGCTAACCAACCTCAGAAAAAATTTGGAGGTTTTTCTATACATTACTATTTTT
  ATATTTGATGACATTTTGTATATACCAAATAAAATGCTACTTCAGATGACAATTCAGAGC
  TTCGCAGGGATTAAAAGGAAGCTGTCCTATTGAGTCCATCATCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004109
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004109.3, NP_004100.1
• RefSeq Size: 3236 bp
• RefSeq ORF: 555 bp
• Locus ID: 2230
• UniProt ID: P10109
• Cytogenetics: 11q22.3
• Domains: fer2
• Gene Summary: This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. [provided by RefSeq, Aug 2011]