C13orf7 (RNF219) (NM_024546) Human Untagged Clone

CAT#: SC108097

RNF219 (untagged)-Human ring finger protein 219 (RNF219)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C13orf7
• Synonyms: C13orf7; RNF219
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024546, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCAGACCGTGCAGAATGTTACATTGTCGCTCACTCTGCCCATCACGTGCCACATTTGCTTGGGGA
  AGGTACGTCAGCCTGTCATATGCATCAACAACCATGTATTTTGTTCGATTTGTATTGATTTGTGGTTGAA
  GAATAATAGCCAGTGTCCAGCTTGCAGAGTCCCCATCACTCCTGAAAATCCTTGCAAAGAAATTATAGGA
  GGAACAAGTGAAAGTGAACCTATGCTAAGCCATACGGTCAGGAAGCATCTTCGGAAAACTAGACTTGAAT
  TACTACACAAAGAATATGAGGACGAAATAGATTGTTTACAGAAAGAAGTAGAAGAGCTTAAGAGTAAAAA
  TCTCAGCTTGGAGTCACAGATCAAAACTATTCTGGATCCTTTAACCTTGGTGCAGGGCAACCAAAATGAA
  GACAAACATCTAGTCACAGATAATCCAAGTAAAATTAACCCAGAAACTGTAGCAGAGTGGAAGAAAAAAC
  TCAGAACAGCTAATGAAATCTATGAAAAAGTGAAAGATGATGTGGATAAGCTAAAGGAGGCAAATAAAAA
  ATTGAAATTGGAAAATGGTGGTCTGGTGAGGGAGAATTTACGACTGAAGGCTGAAGTTGATAACAGATCA
  CCTCAAAAGTTTGGAAGGTTTGCAGTTGCTGCTCTTCAGTCCAAAGTAGAACAGTATGAGCGTGAAACCA
  ATCGCCTCAAGAAAGCCCTGGAACGAAGTGATAAGTATATAGAGGAACTAGAATCTCAAGTTGCACAGCT
  AAAAAATTCAAGTGAAGAGAAAGAAGCTATGAATTCCATTTGCCAGACAGCACTTTCTGCAGATGGCAAA
  GGGAGCAAAGGCAGTGAGGAGGATGTGGTGTCAAAGAATCAAGGCGATAGTGCCAGAAAGCAGCCTGGCT
  CATCCACCTCCAGTTCTTCTCACCTAGCGAAGCCTTCCAGCAGCAGACTGTGTGACACCAGTTCTGCAAG
  GCAGGAAAGTACCAGCAAAGCAGACCTTAACTGTTCTAAGAACAAAGACCTATATCAAGAACAGGTAGAA
  GTAATGTTAGATGTGACAGATACAAGTATGGATACTTATTTGGAAAGAGAATGGGGGAATAAACCAAGTG
  ACTGTGTACCCTACAAAGATGAAGAACTTTATGATCTTCCAGCTCCTTGTACTCCTTTGTCCCTTAGTTG
  CCTTCAGCTCAGTACTCCAGAAAATAGAGAGAGCTCTGTGGTCCAAGCAGGAGGTTCCAAAAAGCACTCA
  AACCATCTCAGAAAATTGGTGTTTGATGATTTTTGTGATTCTTCAAATGTTTCTAATAAAGATTCTTCAG
  AAGATGATATAAGTAGAAGTGAAAATGAAAAGAAATCAGAATGTTTTTCTTCCCCAAAGACAGGATTTTG
  GGACTGTTGTTCCACAAGCTATGCCCAAAACTTAGATTTTGAAAGTTCAGAGGGGAACACGATAGCAAAT
  TCTGTTGGAGAAATATCTTCAAAATTGAGTGAGAAATCAGGCTTATGTTTATCCAAAAGGTTGAATTCTA
  TTCGCTCTTTTGAAATGAACCGGACAAGAACATCCAGTGAAGCATCGATGGATGCTGCTTACCTTGACAA
  AATCTCTGAGTTGGATTCAATGATGTCAGAGTCAGACAACAGCAAGAGCCCTTGTAATAACGGTTTTAAG
  TCACTGGATTTGGATGGGTTATCAAAGTCATCTCAAGGCAGTGAATTTCTTGAGGAACCTGATAAGTTGG
  AAGAAAAAACTGAGCTAAACCTTTCCAAAGGTTCTCTAACTAATGATCAGTTAGAAAATGGAAGTGAATG
  GAAACCCACTTCTTTTTTTCTCCTCTCTCCATCTGACCAAGAAATGAATGAAGATTTTTCACTCCATTCC
  AGTTCTTGTCCAGTAACTAATGAAATCAAACCCCCAAGCTGCTTGTTTCAGACAGAGTTTTCCCAGGGCA
  TTTTGTTAAGCAGTTCACATCGACTATTTGAAGATCAAAGATTTGGGTCATCTTTGTTTAAGATGTCCTC
  AGAGATGCACAGTCTTCATAACCACCTTCAGTCTCCTTGGTCTACTTCCTTTGTGCCTGAAAAGAGGAAT
  AAAAATGTGAATCAATCAACAAAAAGAAAAATCCAGAGCAGCCTTTCCAGTGCCAGCCCATCAAAAGCAA
  CTAAAAGTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024546 unedited
  ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTGCAGTTGCTGCTCTTCAGTCC
  AAAGTAGAACAGTATGAGCGTGAAACCAATCGCCTCAAGAAAGCCCTGGAACGAAGTGAT
  AAGTATATAGAGGAACTAGAATCTCAAGTTGCACAGCTAAAAAATTCAAGTGAAGAGAAA
  GAAGCTATGAATTCCATTTGCCAGACAGCACTTTCTGCAGATGGCAAAGGGAGCAAAGGC
  AGTGAGGAGGATGTGGTGTCAAAGAATCAAGGCGATAGTGCCAGAAAGCAGCCTGGCTCA
  TCCACCTCCAGTTCTTCTCACCTAGCGAAGCCTTCCAGCAGCAGACTGTGTGACACCAGT
  TCTGCAAGGCAGGAAAGTACCAGCAAAGCAGACCTTAACTGTTCTAAGAACAAAGACCTA
  TATCAAGAACAGGTAGAAGTAATGTTAGATGTGACAGATACAAGTATGGATACTTATTTG
  GAAAGAGAATGGGGGAATAAACCAAGTGACTGTGTACCCTACAAAGATGAAGAACTTTAT
  GATCTTCCAGCTCCTTGTACTCCTTTGTCCCTTAGTTGCCTTCAGCTCAGTACTCCAGAA
  AATAGAGAGAGCTCTGTGGTCCAAGCAGGAGGTTCCAAAAAGCACTCAAACCATCTCAGA
  ANATTGGTGTTTGATGATTTTTGTGATTCTTCANATGTTTCTAAATAAGATTCTTCAGAA
  GATGATANTAGTAGAAGTGAAAATGAAAAGAAATCAGAATGGTTTTTCTCCCCAAGACAG
  GATTTGGGACTGGTNNGTCACAGCTATGCCANACTTAATTTTGAAGTCAGAGGGACACGA
  TGCAAATCTGTGGAGAAATCTCAAATGATGAGAACAGCTATGTATNCAAGGNTGATCTAT
  CCTCTTTGATGACCGCAGACATCAGGACATGAGTGCTGTACTGCAAACTTGATGATCAGA
  GGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024546 unedited
  GACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTCAGGGCTTATGACA
  TTCTTTATTCAATTCACATAGAAAAAGCATGCAGTATTAATGTAAAACAGTACAATATTA
  ATGTAAAATGTTCAGTGCACATTAAACAGCATACATACCCATTTTTAAAGACCTATATAG
  GCATACCAAATACGTTTAGAACAATACACTTTTTCAGAGCCTAAATTAAAAATTGTGCTT
  ACCTCTTACCTATCTTCACCCCCTCAACACTCTTCACAGAAAAGTTTTGTCCTACATAAA
  AGATATTCTATCAGCCAACTGAAACCTCTTTTTCTTAAGTATGGAAAACACAGCAAGCAA
  AAATGCTACCATGCATAGTTTCCACAAAGAACAGGAACATGCAAACAAGAAACATACTAC
  TCAAAAGAAAACTCCCCTGGAATGCAAGTGGATCAAGAACTTGGCGATGAGCTCTTTCAA
  ACCTGTTACATCTGGAACAATGAAGCTATGATGTTTTAGGTTCCTCTAAACCCAAGTGCT
  CCATGCCTTCTTTCCATAGTATGCTAAATTTCTGATTTTACACACATACACACACACGCA
  GAGGAGAAAATGTAAGCTACCTAACATAAAACAAATGTCTTCATATAGAAAATGTTNTCT
  TTCATACTAGCAGAACATTTTCACAAAACTGGACAAAACACAATGCTAAAAAATGCAAAC
  ATATACATAAAACCTAAAAAAAGAAAGTAAATCAATATTGGACTAAGGCTAGAGTGCTAT
  TTCTCCATCATGCCCCTGCAGAAAACTAGGCCATCACTAAACATAAATAAAAAGGGACAG
  GGTANAATANTTTCCACCCTGGGAAAAAGCTTNAACTCAGTGTCTTATTGAGCAGTGTAT
  AGACAACACTAAAAGTTTATTTTAGAAATCCANNGTCTGAGACAGTTGGGGCTTATTCAC
  AGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024546
• Insert Size: 2890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024546.1, NP_078822.1
• RefSeq Size: 3552 bp
• RefSeq ORF: 3552 bp
• Locus ID: 79596
• UniProt ID: Q5W0B1
• Cytogenetics: 13q31.1
• Gene Summary: E3 ubiquitin ligase essential for DNA replication origin activation during S phase (PubMed:31160578). Acts as a replication origin selector which selects the origins to be fired and catalyzes the multi-mono-ubiquitination of a subset of chromatin-bound ORC3 and ORC5 during S-phase (PubMed:31160578).[UniProtKB/Swiss-Prot Function]