C13orf7 (RNF219) (NM_024546) Human Tagged ORF Clone

CAT#: RC216309

RNF219 (Myc-DDK-tagged)-Human ring finger protein 219 (RNF219)

Product Images

Circular map for RC216309

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C13orf7
• Synonyms: C13orf7; RNF219
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216309 representing NM_024546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGACCGTGCAGAATGTTACATTGTCGCTCACTCTGCCCATCACGTGCCACATTTGCTTGGGGA
  AGGTACGTCAGCCTGTCATATGCATCAACAACCATGTATTTTGTTCGATTTGTATTGATTTGTGGTTGAA
  GAATAATAGCCAGTGTCCAGCTTGCAGAGTCCCCATCACTCCTGAAAATCCTTGCAAAGAAATTATAGGA
  GGAACAAGTGAAAGTGAACCTATGCTAAGCCATACGGTCAGGAAGCATCTTCGGAAAACTAGACTTGAAT
  TACTACACAAAGAATATGAGGACGAAATAGATTGTTTACAGAAAGAAGTAGAAGAGCTTAAGAGTAAAAA
  TCTCAGCTTGGAGTCACAGATCAAAACTATTCTGGATCCTTTAACCTTGGTGCAGGGCAACCAAAATGAA
  GACAAACATCTAGTCACAGATAATCCAAGTAAAATTAACCCAGAAACTGTAGCAGAGTGGAAGAAAAAAC
  TCAGAACAGCTAATGAAATCTATGAAAAAGTGAAAGATGATGTGGATAAGCTAAAGGAGGCAAATAAAAA
  ATTGAAATTGGAAAATGGTGGTCTGGTGAGGGAGAATTTACGACTGAAGGCTGAAGTTGATAACAGATCA
  CCTCAAAAGTTTGGAAGGTTTGCAGTTGCTGCTCTTCAGTCCAAAGTAGAACAGTATGAGCGTGAAACCA
  ATCGCCTCAAGAAAGCCCTGGAACGAAGTGATAAGTATATAGAGGAACTAGAATCTCAAGTTGCACAGCT
  AAAAAATTCAAGTGAAGAGAAAGAAGCTATGAATTCCATTTGCCAGACAGCACTTTCTGCAGATGGCAAA
  GGGAGCAAAGGCAGTGAGGAGGATGTGGTGTCAAAGAATCAAGGCGATAGTGCCAGAAAGCAGCCTGGCT
  CATCCACCTCCAGTTCTTCTCACCTAGCGAAGCCTTCCAGCAGCAGACTGTGTGACACCAGTTCTGCAAG
  GCAGGAAAGTACCAGCAAAGCAGACCTTAACTGTTCTAAGAACAAAGACCTATATCAAGAACAGGTAGAA
  GTAATGTTAGATGTGACAGATACAAGTATGGATACTTATTTGGAAAGAGAATGGGGGAATAAACCAAGTG
  ACTGTGTACCCTACAAAGATGAAGAACTTTATGATCTTCCAGCTCCTTGTACTCCTTTGTCCCTTAGTTG
  CCTTCAGCTCAGTACTCCAGAAAATAGAGAGAGCTCTGTGGTCCAAGCAGGAGGTTCCAAAAAGCACTCA
  AACCATCTCAGAAAATTGGTGTTTGATGATTTTTGTGATTCTTCAAATGTTTCTAATAAAGATTCTTCAG
  AAGATGATATAAGTAGAAGTGAAAATGAAAAGAAATCAGAATGTTTTTCTTCCCCAAAGACAGGATTTTG
  GGACTGTTGTTCCACAAGCTATGCCCAAAACTTAGATTTTGAAAGTTCAGAGGGGAACACGATAGCAAAT
  TCTGTTGGAGAAATATCTTCAAAATTGAGTGAGAAATCAGGCTTATGTTTATCCAAAAGGTTGAATTCTA
  TTCGCTCTTTTGAAATGAACCGGACAAGAACATCCAGTGAAGCATCGATGGATGCTGCTTACCTTGACAA
  AATCTCTGAGTTGGATTCAATGATGTCAGAGTCAGACAACAGCAAGAGCCCTTGTAATAACGGTTTTAAG
  TCACTGGATTTGGATGGGTTATCAAAGTCATCTCAAGGCAGTGAATTTCTTGAGGAACCTGATAAGTTGG
  AAGAAAAAACTGAGCTAAACCTTTCCAAAGGTTCTCTAACTAATGATCAGTTAGAAAATGGAAGTGAATG
  GAAACCCACTTCTTTTTTTCTCCTCTCTCCATCTGACCAAGAAATGAATGAAGATTTTTCACTCCATTCC
  AGTTCTTGTCCAGTAACTAATGAAATCAAACCCCCAAGCTGCTTGTTTCAGACAGAGTTTTCCCAGGGCA
  TTTTGTTAAGCAGTTCACATCGACTATTTGAAGATCAAAGATTTGGGTCATCTTTGTTTAAGATGTCCTC
  AGAGATGCACAGTCTTCATAACCACCTTCAGTCTCCTTGGTCTACTTCCTTTGTGCCTGAAAAGAGGAAT
  AAAAATGTGAATCAATCAACAAAAAGAAAAATCCAGAGCAGCCTTTCCAGTGCCAGCCCATCAAAAGCAA
  CTAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216309 representing NM_024546
  Red=Cloning site Green=Tags(s)
  
  MAQTVQNVTLSLTLPITCHICLGKVRQPVICINNHVFCSICIDLWLKNNSQCPACRVPITPENPCKEIIG
  GTSESEPMLSHTVRKHLRKTRLELLHKEYEDEIDCLQKEVEELKSKNLSLESQIKTILDPLTLVQGNQNE
  DKHLVTDNPSKINPETVAEWKKKLRTANEIYEKVKDDVDKLKEANKKLKLENGGLVRENLRLKAEVDNRS
  PQKFGRFAVAALQSKVEQYERETNRLKKALERSDKYIEELESQVAQLKNSSEEKEAMNSICQTALSADGK
  GSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSSSRLCDTSSARQESTSKADLNCSKNKDLYQEQVE
  VMLDVTDTSMDTYLEREWGNKPSDCVPYKDEELYDLPAPCTPLSLSCLQLSTPENRESSVVQAGGSKKHS
  NHLRKLVFDDFCDSSNVSNKDSSEDDISRSENEKKSECFSSPKTGFWDCCSTSYAQNLDFESSEGNTIAN
  SVGEISSKLSEKSGLCLSKRLNSIRSFEMNRTRTSSEASMDAAYLDKISELDSMMSESDNSKSPCNNGFK
  SLDLDGLSKSSQGSEFLEEPDKLEEKTELNLSKGSLTNDQLENGSEWKPTSFFLLSPSDQEMNEDFSLHS
  SSCPVTNEIKPPSCLFQTEFSQGILLSSSHRLFEDQRFGSSLFKMSSEMHSLHNHLQSPWSTSFVPEKRN
  KNVNQSTKRKIQSSLSSASPSKATKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024546
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024546.4
• RefSeq Size: 3552 bp
• RefSeq ORF: 2181 bp
• Locus ID: 79596
• UniProt ID: Q5W0B1
• Cytogenetics: 13q31.1
• MW: 80.9 kDa
• Gene Summary: E3 ubiquitin ligase essential for DNA replication origin activation during S phase (PubMed:31160578). Acts as a replication origin selector which selects the origins to be fired and catalyzes the multi-mono-ubiquitination of a subset of chromatin-bound ORC3 and ORC5 during S-phase (PubMed:31160578).[UniProtKB/Swiss-Prot Function]