SNAPIN (NM_012437) Human Untagged Clone

CAT#: SC108055

SNAPIN (untagged)-Human SNAP-associated protein (SNAPIN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAPIN
• Synonyms: BLOC1S7; BLOS7; BORCS3; SNAPAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108055 sequence for NM_012437 edited (data generated by NextGen Sequencing)
  ATGGCGGGGGCTGGTTCCGCCGCTGTATCGGGGGCAGGGACCCCGGTGGCGGGGCCCACA
  GGCCGCGACCTTTTCGCCGAAGGGCTGCTGGAGTTCCTGCGACCCGCTGTGCAGCAGCTC
  GACTCTCACGTACACGCCGTCAGAGAGAGCCAGGTAGAGCTCCGGGAACAAATTGACAAC
  CTAGCCACAGAACTGTGCCGCATAAATGAGGATCAGAAGGTGGCCCTGGATCTTGACCCC
  TATGTTAAGAAGCTACTTAATGCCCGGCGACGCGTTGTCTTGGTTAACAACATTCTACAG
  AATGCTCAGGAACGACTGAGACGGCTAAACCACAGTGTTGCCAAGGAAACAGCCCGCAGG
  AGAGCAATGCTGGATTCGGGAATTTACCCCCCTGGCTCCCCAGGCAAATAA
  
  Clone variation with respect to NM_012437.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012437 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGGTTTCGGGCTTCAGGA
  CAATTCGTGATGGCGGGGGCTGGTTCCGCCGCTGTATCGGGGGCAGGGACCCCGGTGGCG
  GGGCCCACAGGCCGCGACCTTTTCGCCGAAGGCTGCTGGAGTTCCTGCGACCCGCTGTGC
  AGCAGCTCGACTCTCACGTACACGCCGTCAGAGAGAGCCAGGTAGAGCTCCGGGAACAAA
  TTGACAACCTAGCCACAGAACTGTGCCGCATAAATGAGGATCAGAAGGTGGCCCTGGATC
  TTGACCCCTATGTTAAGAAGCTACTTAATGCCCGGCGACGCGTTGTCTTGGTTAACAACA
  TTCTACAGAATGCTCAGGAACGACTGAGACGGCTAAACCACAGTGTTGCCAAGGAAACAG
  CCCGCAGGAGAGCAATGCTGGATTCGGGAATTTACCCCCCTGGCTCCCCAGGCAAATAAC
  AGATGAGCCTATGGACTCAGTAGCACAAGTACTGTTCCCCAGCTGCCTTGTTTCAACAGA
  CATGCAAAGATCCTAGGAGACAGTCCCCATAGACTTTCAGACATTAAAAAGGGAGCCGTA
  CAGTTTGTTTGAAGCACTTCGTCTTACCATTTATGTAGGGGCCCCAGGAAACCTACCCAC
  AGCCAGAATGAGGTTCCCANAGGACTTACANTTAATATGGCTCTGGCTTNCTTTCCANAT
  GAGCTGAGGCCTCTACTTTNTTTTTTAGCTGCATACTTGAGGCTTACCTTCTTAGGACTA
  GTTACCAGAGGGGCTNNCTTTGNNATGTACATGCCTGGTACATGGNCCCTGAAGCATGNC
  CCTACCTGGGACTCTCATTTTCCTGGTAACCTGGGGATTGGAGGGGGAGGCAAAGCAAGG
  AATGACCTTTGCCACCATTTTTATTGNCTGCCTGAAACNAACCATGTGAAAAAACTTTTT
  CAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012437 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAT
  TTAAAGAATTTATGGAATAAAATGATTCTATCAACACTGCTACTGTTTTCAACCCAGGGC
  AATAAAAAAGGGGGGGGACAGAGGTCATTCACTTTGACTTTGCCTGCCCCCTCCAAATCC
  CCAGGGTAAACAGGAAAATGAAAAGTCACAGGTAAAGGACATGCTGTCCAGCCCCATGTA
  ACCAGGCTTGTAACATACAAAGGAACCCCCTTTGGTTAACTAGTCCTGAAAAAGGTAACC
  CTCAAGTTTGCAGTTTAAAAAAAAAAGTAAAGGCCTCAGCTCATTTGTGAAAGGAACCAA
  AACCCTTAATTAATGTAAGTCCTTTGGGAACCTCATTCTGGCTGGGTGTAGGTTTCCTGG
  GGCCCCTACATAAATGGGTAAAACAAAGGGCTTAAAACAAACTGTACGGCTCCCTTTTTA
  ATGTCTGAAAGTCTATGGGGACTGTCTCCTAGGATCTTTGCATGTCTGTTAAAACAAGGC
  ACCTGGGGAACAGTACTTGGGCTACTGAGTCCATAGGCTCATCTGTTATTTGCCTGGGGA
  GCCAGGGGGGTAAATTCCCAAATCCAGCATTGCTCTCCTGCGGGCTGTTTCCTTGGCAAC
  ACTGTGGTTTACCCGTCTAAGTCGTTCCTGACCATTCTGTAGAATGTTGTTAACCAAGAC
  AACGCGTCGCCGGCCATTAAGTAGCTTCTTACCTAGGGGTCAAGATCCAGGCCCCCTTCT
  GATCCTAATTATGCCGCACAGTTCTGGGGCTAGGTTGAAATTTGTTCCCGAACTCTACCT
  GNCTCTCTTGACGGGTGTACCTGAAATCAAACTCTGCACAAGGGTCCCAGGACTCCACAC
  CCTTCGAGAAGAGTCCCGCCGTGGGCCCCCACCCGGTCCCTGCCTCGACAAGGNGGACCA
  ACCCCCCATACCATGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012437
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012437.3, NP_036569.1
• RefSeq Size: 988 bp
• RefSeq ORF: 411 bp
• Locus ID: 23557
• UniProt ID: O95295
• Cytogenetics: 1q21.3
• Gene Summary: The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein.