RBCK1 (NM_031229) Human Untagged Clone

CAT#: SC108003

RBCK1 (untagged)-Human RanBP-type and C3HC4-type zinc finger containing 1 (RBCK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBCK1
• Synonyms: C20orf18; HOIL-1; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; UBCE7IP3; XAP3; XAP4; ZRANB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_031229 edited
  GAATTCGGCACGAGGCGCTCCCCTTGCGAAGAACTGGGGCCTCCCGGGAGGAGAGAGGGC
  TTTGCCTTGAAACCCGGGACGCCAGGGGCGCTCCCGCAAGTGGGGGTCCTCCGGGACTTG
  GAACGCCCCGGCTGGGTGGTGTCCGGGCGTCCTTTCCCCGCTTCTTCCCACCTCGGCTGG
  TCCCGTTTCCTCCTGCGCCCAGTGCGGACCTGTCTCGGCGCCCGCTGCCCTCTCACCGCC
  CCACGCAGGATCCCGGCCTGGTCACCGGGCAGTGTGATGCTTCCCGACTGCCGCGGGGAC
  AGCGAGGCACACACAGGGCTTGGGCCGCGCCGGAGGCCACACGGCCTGGCTGAGTTGCTC
  CTGGTCTCCCGCCTCTCCCAGGCGACCCGGAGGTAGCATTTCCCAGGAGGCACGGTCCCC
  CCCAGGGGGATGGGCACAGCCACGCCAGATGGACGAGAAGACCAAGAAAGCAGAGGAAAT
  GGCCCTGAGCCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTGC
  CATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCTGAGGTCTCCCC
  AACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGCTCAGATGCACACCGTCACCAT
  CTGGCTCACAGTGCGCCCTGATATGACAGTGGCGTCTCTCAAGGACATGGTTTTTCTGGA
  CTATGGCTTCCCACCAGTCTTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCA
  GGAGACCCTGCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGCT
  GTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGGCAGCTGCGGAT
  GCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCCGCGGGGCCCTCTGGAGCCAGG
  CCCCCCAAAGCCCGGGGTCCCCCAGGAACCCGGACGGGGGCAGCCAGATGCAGTGCCTGA
  GCCCCCACCGGTGGGCTGGCAGTGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCC
  TGGCTGTGAGATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACCA
  GCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTGCGTCAGTACCA
  GCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCAGCACGTCCAGCTGGACCAGAG
  GAGCCTGGTGCTGAACACGGAGCCCGCCGAGTGCCCCGTGTGCTACTCGGTGCTGGCGCC
  CGGCGAGGCCGTGGTGCTGCGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGG
  CACCATCCGCAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACTC
  GTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCTGAGGATTACCA
  GCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCGCAGTGCCTTCAGCTACCATTG
  CAAGACCCCAGATTGCAAGGGATGGTGCTTCTTTGAGGATGATGTCAATGAGTTCACCTG
  CCCTGTGTGTTTCCACGTCAACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTG
  CAAGGAGTATCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGAC
  GACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGCCCCCAGTGCCA
  GATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCGCTGCACCGTCTGCCACACCGA
  GATCTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCTGGGGGCCCAGGAGACACCAGCGG
  GGGCTGCCGCTGCAGGGTAAATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTG
  AGCTAAAGATGGTGGGGCCACATGCTGACCCAGCCCCACATCCACATTCTGTTAGAATGT
  AGCTCAGGGAGCTTCGTGGACGGCCTTGCTTGCTGTAGCGTTGTAGGGGCCCTGCCTGCA
  CTGCGGTTGTCCACGGTCACATCTGCCCCAGTGCCTTTGTCCTTCCCTTGGGGCTTGCCG
  GCCAGACTTCTCTCCCCTGCGGCTCCCACCTCTGCCTGACCCCAGCCTTAAACATAGCCC
  CTGGCTAGAGGCCTTGCTGGGTGGAGCCTCTGTGTGACTCCATACTCCTCCCACCACAAC
  ACTCATCTGTCAAACACCAAGCACTCTCAGCCTCCCCGCCTTCAGCTGTCAGCTTTCTGG
  GGCTAACTTCTCTGCCTTTGTGGTTGGAGGCCTGAGGCCTCTTGGAACTCTTGCTAACCT
  GTTCAGAGCCAGGAAGGAGACTGCACAGTTTTGAAAGCACAGCCCGTCAGGTCCGGCTCT
  GCGTCTCCCTCTCTGCAGCCTGTGTAAGCTATTATAATTAAAATGGTTTTCCGGGAAAAA
  AAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031229 unedited
  TCAAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGCTCCCCTT
  GCGAAGACTGGGGCCTCCCGGGAGGAGAGAGGGCTTTGCCTTGAAACCCGGGACGCCAGG
  GGCGCTCCCGCAAGTGGGGGTCCTCCGGGACTTGGAACGCCCCGGCTGGGTGGTGTCCGG
  GCGTCCTTTCCCCGCTTCTTCCCACCTCGGCTGGTCCCGTTTCCTCCTGCGCCCAGTGCG
  GACCTGTCTCGGCGCCCGCTGCCCTCTCACCGCCCCACGCAGGATCCCGGCCTGGTCACC
  GGGCAGTGTGATGCTTCCCGACTGCCGCGGGGACAGCGAGGCACACACAGGGCTTGGGCC
  GCGCCGGAGGCCACACGGCCTGGCTGAGTTGCTCCTGGTCTCCCGCCTCTCCCAGGCGAC
  CCGGAGGTAGCATTTCCCAGGAGGCACGGTCCCCCCCAGGGGGATGGGCACAGCCACGCC
  AGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAGCCTCACCCGAGCAGTGG
  CGGGCGGGGATGAACAGGTGGCAATGAAGTGTGCCATCTGGCTGGCAGAGCAACGGGTGC
  CCCTGAGTGTGCAACTGAAGCCTGAGGTCTCCCCAACGCAGGACATCANGCTGTGGGTGA
  GCGTGGAGGATGCTCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGA
  CAGTGGCGTCTCTCAAGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTCTTGCAGCA
  GTGGGTGATTGGGCAGCGGCTGGCACGAGAACAGGAGAACCTGCACTTCCATGGGGTGCG
  GCAGAATGGGGACAGTGCCTACCTTATCTGCTGTCAGNCCCGCACACTCCTTCACCTCAG
  AACTGCACCGGNNAGCGCACTGCCGATGCTGAANAATCTGGCTTCAAGGACTTAGCTGCA
  GCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031229 unedited
  CCGCATTTAGNGTCGAGTTTTTTTTTTTTTTTTTTCCCGGAAAACCATTTTAATTATAAT
  AGCTTACACAGGCTGCAGAGAGGGAGACGCAGAGCCGGACCTGACGGGCTGTGCTTTCAA
  AACTGTGCAGTCTCCTTCCTGGCTCTGAACAGGTTAGCAAGAGTTCCAAGAGGCCTCAGG
  CCTCCAACCACAAAGGCAGAGAAGTTATCCCCAGAAAGCTGACAGCTGAAGGCGGGGAGG
  CTGAAAGTGCTTGGTGTTTGACAGATGAGTGTTGTGGTGGGAGGAGTATGGAGTCACACA
  GAGGCTCCACCCAGCAAGGCCTCTAGCCAGGGGCTATGTTTAAGGCTGGGGTCAGGCAGA
  GGTGGGAGCCGCAGGGGAGAGAAGTCTGGCCGGCAAGCCCCAAGGGAAGGACAAAGGCAC
  TGGGGCAGATGTGACCGTGGACAACCGCAGTGCAGGCAGGGCCCCTACAACGCTACAGCA
  AGCAAGGCCGTCCACGAAGCTCCCTGAGCTACATTCTAACAGAATGTGGATGTGGGGCTG
  GGTCANCATGTGGCCCCACCATCTTTAGCTCAGTGGCAGTTCTGACAGCTTGGGTGGCAA
  GGATCCCATTTACCCCTGCAGCGGCAGCCCCCGCTGGTGTCTCCTGGGCCCCCAGGCCCC
  CAGCGTGGGCCCCTTGGTGACCCANCAGATCTCGGTGTGGCAAACCGTGCANNGGATCCA
  GTCCCAGCCGTCCTCCTTTGTACCACGATCTGCACTGGNGGGAGCGCATGGCCTCNCCCT
  GCTGCAAATAACCTTCAAGATCTCTGGCGTTTGCCGGCCAGCCCCATTGTCTGGCCCCCA
  AGCCCAGGCCTCCTGAGACTCCTTGCAGTTATATGCTCATGATGCCCTTCCAACCAGCCA
  TTGACCGGAAAACCCAGGCCAGGGAACTATTGCCTTATCCTCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031229
• Insert Size: 2680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031229.1, NP_112506.1
• RefSeq Size: 2715 bp
• RefSeq ORF: 1503 bp
• Locus ID: 10616
• UniProt ID: Q9BYM8
• Cytogenetics: 20p13
• Domains: RING, zf-RanBP
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.