Aromatase (CYP19A1) (NM_031226) Human Untagged Clone

CAT#: SC107980

CYP19A1 (untagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aromatase
• Synonyms: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_031226 edited
  GGGCGGCCGCGAATTCGGCACGAGGAACCGTGGAGGCAAACAGGAAGGTGAAGAAGAACT
  TATCCTATCAGGACGGAAGGTCCTGTGCTCGGGATCTTCCAGACGTCGCGACTCTAAATT
  GCCCCCTCTGAGGTCAAGGAACACAAGATGGTTTTGGAAATGCTGAACCCGATACATTAT
  AACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTGCCACCATGCCAGTCCTGCTCCTC
  ACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTCAATACCAGGTCCTGGC
  TACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCTGTGGATGGGGATCGGC
  AGTGCCTGCAACTACTACAACCGGGTATATGGAGAATTCATGCGAGTCTGGATCTCTGGA
  GAGGAAACACTCATTATCAGCAAGTCCTCAAGTATGTTCCACATAATGAAGCACAATCAT
  TACAGCTCTCGATTCGGCAGCAAACTTGGGCTGCAGTGCATCGGTATGCATGAGAAAGGC
  ATCATATTTAACAACAATCCAGAGCTCTGGAAAACAACTCGACCCTTCTTTATGAAAGCT
  CTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCTGTGCTGAATCCCTCAAAACACAT
  CTGGACAGGTTGGAGGAGGTGACCAATGAATCGGGCTATGTGGACGTGTTGACCCTTCTG
  CGTCGTGTCATGCTGGACACCTCTAACACGCTCTTCTTGAGGATCCCTTTGGACGAAAGT
  GCTATCGTGGTTAAAATCCAAGGTTATTTTGATGCATGGCAAGCTCTCCTCATCAAACCA
  GACATCTTCTTTAAGATTTCTTGGCTATACAAAAAGTATGAGAAGTCTGTCAAGGATTTG
  AAAGATGCCATAGAAGTTCTGATAGCAGAAAAAAGACGCAGGATTTCCACAGAAGAGAAA
  CTGGAAGAATGTATGGACTTTGCCACTGAGTTGATTTTAGCAGAGAAACGTGGTGACCTG
  ACAAGAGAGAATGTGAACCAGTGCATATTGGAAATGCTGATCGCAGCTCCTGACACCATG
  TCTGTCTCTTTGTTCTTCATGCTATTTCTCATTGCAAAGCACCCTAATGTTGAAGAGGCA
  ATAATAAAGGAAATCCAGACTGTTATTGGTGAGAGAGACATAAAGATTGATGATATACAA
  AAATTAAAAGTGATGGAAAACTTCATTTATGAGAGCATGCGGTACCAGCCTGTCGTGGAC
  TTGGTCATGCGCAAAGCCTTAGAAGATGATGTAATCGATGGCTACCCAGTGAAAAAGGGG
  ACAAACATTATCCTGAATATTGGAAGGATGCACAGACTCGAGTTTTTCCCCAAACCCAAT
  GAATTTACTCTTGAAAATTTTGCAAAGAATGTTCCTTATAGGTACTTTCAGCCATTTGGC
  TTTGGGCCCCGTGGCTGTGCAGGAAAGTACATCGCCATGGTGATGATGAAAGCCATCCTC
  GTTACACTTCTGAGACGATTCCACGTGAAGACATTGCAAGGACAGTGTGTTGAGAGCATA
  CAGAAGATACACGACTTGTCCTTGCACCCAGATGAGACTAAAAACATGCTGGAAATGATC
  TTTACCCCAAGAAACTCAGACAGGTGTCTGGAACACTAGAGAAGGCTGGTCAGTACCCAC
  TCTGGAGCATTTCTCATCAGTAGTTCACATACAAATCATCCATCCTTGCCAATAGTGTCA
  TCCTCACAGTGAACACTCAGTGGCCCATGGCATTTTATAGGCATACCTCCTATGGGTTGT
  CACCAAGCTAGGTGCTATTTGTCATCTGCTCCTGTTCACACCAGAGAACCAGGCTACAAG
  AGAAAAAGCAGAGGCCAAGAGTTTGAGGGAGAAATAGTCGGTGAAGAAACCGTATCCATA
  AAGACCCGATTCCACCAAATGTGCTTTGAGAAGGATAGGCCTTCATTAACAAAATGTATG
  TCTGGTTCCCCAGTAGAGCTCTACTGCCTCAACCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031226 unedited
  CACGAGNAACCGTGGNAGCAAACAGGAAGGTGAAGAAGAACTTATCCTATCAGGACGGAA
  GGTCCTGTGCTCGGGATCTTCCAGACGTCGCGACTCTAAATTGCCCCCTCTGAGGTCAAG
  GAACACAAGATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTG
  CCTGAAGCCATGCCTGCTGCCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTG
  GTGTGGAATTATGAGGGCACATCCTCAATACCAGGTCCTGGCTACTGCATGGGAATTGGA
  CCCCTCATCTCCCACGGCAGATTCCTGTGGATGGGGATCGGCAGTGCCTGCAACTACTAC
  AACCGGGTATATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAACACTCATTATC
  AGCAAGTCCTCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGGC
  AGCAAACTTGGGCTGCAGTGCATCGGTATGCATGAGAGAGGCATCATATTTAACAACAAT
  CCAGAGCTCTGGANAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTT
  GTTCGTATGGTCACAGTCTGTGCTGAATCCCTCANACACATCTGGACAGGTTGGAGGAGG
  TG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031226 unedited
  TATCTTTGGNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGGAACA
  TGCCAAAGGATTTTTATTTGAATTTGAAGTGCATCATGTGAATTTTTTTTTCTACAGCAG
  ACACAGGGCCCTAATAATACTTAACCCAGGCTATTACTAAATTAGTTTGTATTACTTGAT
  GATTATATATAGTTTGTATTACCTGAATCTACAGGTAACACAATGAATTGTAGCACAGGC
  AAGTGGCTGAGGCATAAATCGACAGACTGGGAAAGAATTTCCTCTGATTAAACTTTTGCC
  ACAGACAGATCATATGTAGACAAACAGGAATTAATTGGGCTTAATTCACAGCAAGGGTCA
  AATGCTGAATTTCTAAGCATTTCTCCAAAGACTATGAATGTTGCTTTTCCACCTCCACAG
  AAAACAAAATTAAAGTACTTTAATTCACACTAGCAGGTGGGTTTGGCCCCAGGTACCCTG
  ACATTGGCCTGGTCTTTCTAATCAACTTGAGTGTTTCTGCCCCAGACATAAAAATCCCCT
  TGGGTTGAGGCAGTAGAGCTCTACTGGGGAACCAGACATACATTNTGTTAATGAAGGCCT
  ATCCTTCTCAAAGCACATTTGGTGGAATCGGGTCTTTATGGATACGGNTTCTTCACCGAC
  TATTTCTCCCTCAAACTCTTGGCCTCTGCTTTNTCTCTTGTAGCCTGGNTCTCTGGGTGT
  GACAGGAGCAGATGACAAATAGCACCTAGCTTGGGTGACACCCCATAGAGGTATGCCTAT
  NAAATGCCATGGGCCACTGANNTGTCACTGTNGAGATGACACTATTGGCAGGNATGGATG
  ATTTNGNATGTGAACTACTGATGAGAAATGCTNNCAGGTGGGTACTGACCAGNCTTTNCT
  AGTGTTCCAGACACCTGNCTGATTNCTTGGGGTAAAGATCATTNTCAGCATGTNTTTTAG
  TCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031226
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031226.1, NP_112503.1
• RefSeq Size: 3116 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1588
• UniProt ID: P11511
• Cytogenetics: 15q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]
  Transcript Variant: This variant (2) represents the use of an alternate promoter and contains alternate 5' exons (I.1 and 2a), compared to variant 4. All variants encode the same protein. The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 19577618). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.