FZD3 (NM_017412) Human Untagged Clone

SKU SC107940

FZD3 (untagged)-Human frizzled family receptor 3 (FZD3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FZD3
• Synonyms: Fz-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107940 sequence for NM_017412 edited (data generated by NextGen Sequencing); ATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTTCATGGGGCATATA; GGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTGCCAAGATTTGCCT; TATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACAGACAGCAGCTTTG; GCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGATTTCCGGCCTTTT; CTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCACACTTCCCTGTCGT; AGGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGATGTTTGGTGTTCCT; TGGCCTGAAGATATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCATATCCTCGACTT; GTGGATCTGAATTTAGCTGGAGAACCAACTGAAGGAGCCCCAGTGGCAGTGCAGAGAGAC; TATGGTTTTTGGTGTCCCCGAGAGTTAAAAATTGATCCTGATCTGGGTTATTCTTTTCTG; CATGTGCGTGATTGTTCACCTCCTTGTCCAAATATGTACTTCAGAAGAGAAGAACTGTCA; TTTGCTCGCTATTTCATAGGATTGATTTCAATCATTTGCCTCTCGGCCACATTGTTTACT; TTTTTAACTTTTTTGATTGATGTCACAAGATTCCGTTATCCTGAAAGGCCTATTATATTT; TATGCAGTCTGCTACATGATGGTATCCTTAATTTTCTTCATTGGATTTTTGCTTGAAGAT; CGAGTAGCCTGCAATGCATCCATCCCTGCACAATATAAGGCTTCCACAGTGACACAAGGA; TCTCATAATAAAGCCTGTACCATGCTTTTTATGATACTCTATTTTTTTACTATGGCTGGC; AGTGTATGGTGGGTAATTCTTACCATCACATGGTTTTTAGCAGCTGTGCCAAAGTGGGGT; AGTGAAGCTATTGAGAAGAAAGCATTGCTGTTTCACGCCAGTGCATGGGGCATCCCCGGA; ACTCTAACCATCATCCTTTTAGCGATGAATAAAATTGAAGGTGACAATATTAGTGGCGTG; TGTTTTGTTGGCCTCTACGATGTTGATGCATTGAGATATTTTGTTCTTGCTCCCCTCTGC; CTGTATGTGGTAGTTGGGGTTTCTCTCCTCTTAGCTGGCATTATATCCCTAAACAGAGTT; CGAATTGAGATTCCATTAGAAAAGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGG; ATCGGTGTTTTCAGCATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTT; TATGAGCAAGCTTACCGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAA; TATCACATTCCATGTCCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTT; CTGATGAAATACCTGATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGC; AAAAAGACATGCTTTGAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTG; AATGAGAGCCGACAGGTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCA; AATACTCCTATCATAAGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCT; TCTTCAACTCAGCTGGCTATGGTGGATGATCAAAGAAGCAAAGCAGGAAGCATCCACAGC; AAAGTGAGCAGCTACCACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGC; AGTTACAGAGGAATGGAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCAC; TCCAGGCATAGTAGTTCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGAT; CTCAGTAATAATCCCATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAA; GAAGATGGAACCAGTGCTTAA; ; Clone variation with respect to NM_017412.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017412 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGATATGTTGGCCAAAT; GTGCCCCATGTAATAAAATGAAAAGAAGAGACAAGATGATGTCATTTTCCCATATTGTGA; AACCAAAAACAAACGCCTTTTGTGAGACCAAGCTAACAAACCTCTGACGGTGCGAAGAGT; ATTTAACTGTTTGAAGAATTTAACAGTAAGATACAGAAGAAGTACCTTCGAGCTGAGACC; TGCAGGTGTATAAATATCTAAAATACATATTGAATAGGCCTGATCATCTGAATCTCCTTC; AGACCCAGGAAGGATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTT; CATGGGGCATATAGGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTG; CCAAGATTTGCCTTATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACA; GACAGCAGCTTTGGCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGA; TTTCCGGCCTTTTCTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCAC; ACTTCCCTGTCGTANGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGAT; GTTTGGTGTTCCTTGGCCTGAAGAATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCA; TATCCTCGACTTGTGGATCTGAATNTAGCTGGAGAACCAACTGANAGAGCCCCAGTGGCA; GTGCAAAGAGACTATGGTTTTTGGTGTCCCCGAGAGTTAAAAATGATCCTGATCTGGGNT; ATTCTTTTCTGCATGTGCGTGATTGGTCACCTTCTTGTCAATATGTACTTCAAAAGAGAA; GACTGTCATTTGCTCGCTTTNCATAGGNAATGATTCATCATTTGCTCTCGGNACCATGTT; ACTTTTTAACTTTTGATGATGCACAGATCCGT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_017412 unedited; TGGTTGGTACTCATCTTGACTGGCATTATATCCCTAAACATAGTTCGAATTGAGATTCCA; TTATAAATGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGGTTCGGTGTTTTCAGC; ATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTTTATGAGCAAGCTTAC; CGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAATATCACATTCCATGT; CCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTTCTGATGAAATACCTG; ATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGCAAAAAGACATGCTTT; GAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTGAATGAGAGCCGACAG; GTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCAAATACTCCTATCATA; AGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCTTCTTCAACTCAGCTG; GCTATGGTGGATGATCAAAGAAGCAAAGCATGAAGCATCCACAGCATAGTGAGCAGCTAC; CACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGCAGTTACAGAGGAATG; GAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCACTCCCAGCATAGTAGT; TCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGATCCTCAGTATAATCCC; ATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAAAGAGATGGAACCAGT; NGCTNAATTNGNNCTGNCTAAAGGAGGAAATCTGGTGCTGNTTAAAAAAGCAGATTTATT; CCTTGNCTTTTGCATGACTGATAGCTGGTACTNACAAGTAACATGCTTTCAGTCAAGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017412
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017412.2, NP_059108.1
• RefSeq Size: 3933 bp
• RefSeq ORF: 2001 bp
• Locus ID: 7976
• UniProt ID: Q9NPG1
• Cytogenetics: 8p21.1
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Summary: This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.