beta Catenin (CTNNB1) (NM_001904) Human Untagged Clone
CTNNB1 (untagged)-Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1
|Product Name||beta Catenin (CTNNB1) (NM_001904) Human Untagged Clone|
|Type||Human Untagged Clone|
|Synonyms||armadillo; CTNNB; EVR7; MRD19; NEDSDV|
|E. coli Selection||Ampicillin (100 ug/mL)|
>NCBI ORF sequence for NM_001904, the custom clone sequence may differ by one or more nucleotides
|Insert Size||4000 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3720 bp|
|RefSeq ORF||987 bp|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors|
|Protein Pathways||Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway|
|Gene Summary||The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same isoform (1).
|The use of this cDNA Clones has been cited in the following citations:|
Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy
,Nakamura, M;Liu, T;Husain, S;Zhai, P;Warren, JS;Hsu, CP;Matsuda, T;Phiel, CJ;Cox, JE;Tian, B;Li, H;Sadoshima, J;,
Cell Metab. 2019
,PubMed ID 30745182
Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro
,Wahab, R;Kaushik, N;Khan, F;Kaushik, NK;Lee, SJ;Choi, EH;Al-Khedhairy, AA;,
Int J Nanomedicine 2019
,PubMed ID 30863050
CXCL9 promotes the progression of diffuse large B-cell lymphoma through up-regulating β-catenin
,Ruiduo, C;Ying, D;Qiwei, W;,
Biomed. Pharmacother. 2018
,PubMed ID 30130730
Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting β-catenin by activating PKM2 and inactivating PTEN
,Zheng, Q;Lin, Z;Xu, J;Lu, Y;Meng, Q;Wang, C;Yang, Y;Xin, X;Li, X;Pu, H;Gui, X;Li, T;Xiong, W;Lu, D;,
Cell Death Dis 2018
,PubMed ID 29449541
Functional inhibition of ß-catenin-mediated Wnt signalling by intracellular VHH antibodies
,Newnham, LE;Wright, MJ;Holdsworth, G;Kostarelos, K;Robinson, MK;Rabbitts, TH;Lawson, AD;,
MAbs Dec 2014
,PubMed ID 25524068
Altered Protease-Activated Receptor-1 Expression and Signaling in a Malignant Pleural Mesothelioma Cell Line, NCI-H28, with Homozygous Deletion of the ß-Catenin Gene
,Fazzini, A;D'Antongiovanni, V;Giusti, L;Da Valle, Y;Ciregia, F;Piano, I;Caputo, A;D'Ursi, AM;Gargini, C;Lucacchini, A;Mazzoni, MR;,
PLoS ONE Nov 2014
,PubMed ID 25364818
Podocalyxin Promotes Glioblastoma Multiforme Cell Invasion and Proliferation via ß-Catenin Signaling
,Liu, Y;Jiang, YG;,
PLoS ONE Oct 2014
,PubMed ID 25349993
ß-Catenin Activates the HOXA10 and CDX4 Genes in Myeloid Progenitor Cells
,Ling Bei, Chirag Shah, Hao Wang, Weiqi Huang, Rupali Roy, and Elizabeth A. Eklund,
J. Biol. Chem., Nov 2012; 287: 39589 - 39601.
Negative Cross-talk between Calcium-sensing Receptor and ß-Catenin Signaling Systems in Colonic Epithelium
,Osvaldo Rey, Wenhan Chang, Daniel Bikle, Nora Rozengurt, Steven H. Young, and Enrique Rozengurt,
J. Biol. Chem., Jan 2012; 287: 1158 - 1167.
Protein kinase C is a positive modulator of canonical Wnt signaling pathway in tumoral colon cell lines
,Luis Bernardo Luna-Ulloa, José G. Hernández-Maqueda, Paula Santoyo-Ramos, M.Cristina Castañeda-Patlán, and Martha Robles-Flores,
Carcinogenesis, Nov 2011; 32: 1615 - 1624.
The Imprinted Gene PEG3 Inhibits Wnt Signaling and Regulates Glioma Growth
,Xiuli Jiang, Yi Yu, Hong Wei Yang, Nathalie Y. R. Agar, Laura Frado, and Mark D. Johnson,
J. Biol. Chem., Mar 2010; 285: 8472 - 8480
Targeting PKC: a novel role for beta-catenin in ER stress and apoptotic signaling
,Marc S. Raab, Iris Breitkreutz, Giovanni Tonon, Jing Zhang, Patrick J. Hayden, Thu Nguyen, Johannes H. Fruehauf, Boris K. Lin, Dharminder Chauhan, Teru Hideshima, Nikhil C. Munshi, Kenneth C. Anderson, and Klaus Podar,
Blood, Feb 2009; 113: 1513 - 1521.
|cDNA Clone Resources|
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|RG208947||CTNNB1 (GFP-tagged) - Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1||
|RC208947L1||Lenti ORF clone of Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1, Myc-DDK-tagged||
|RC208947L2||Lenti ORF clone of Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1, mGFP tagged||
|RC208947L3||Lenti ORF clone of Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1, Myc-DDK-tagged||
|RC208947L4||Lenti ORF clone of Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1, mGFP tagged||