HNRNPD (NM_031370) Human Untagged Clone

CAT#: SC107836

HNRNPD (untagged)-Human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNRNPD
• Synonyms: AUF1; AUF1A; hnRNPD0; HNRPD; P37
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107836 sequence for NM_031370 edited (data generated by NextGen Sequencing)
  ATGTCGGAGGAGCAGTTCGGCGGGGACGGGGCGGCGGCAGCGGCAACGGCGGCGGTAGGC
  GGCTCGGCGGGCGAGCAGGAGGGAGCCATGGTGGCGGCGACACAGGGGGCAGCGGCGGCG
  GCGGGAAGCGGAGCCGGGACCGGGGGCGGAACCGCGTCTGGAGGCACCGAAGGGGGCAGC
  GCCGAGTCGGAGGGGGCGAAGATTGACGCCAGTAAGAACGAGGAGGATGAAGGCCATTCA
  AACTCCTCCCCACGACACTCTGAAGCAGCGACGGCACAGCGGGAAGAATGGAAAATGTTT
  ATAGGAGGCCTTAGCTGGGACACTACAAAGAAAGATCTGAAGGACTACTTTTCCAAATTT
  GGTGAAGTTGTAGACTGCACTCTGAAGTTAGATCCTATCACAGGGCGATCAAGGGGTTTT
  GGCTTTGTGCTATTTAAAGAATCGGAGAGTGTAGATAAGGTCATGGATCAAAAAGAACAT
  AAATTGAATGGGAAGGTGATTGATCCTAAAAGGGCCAAAGCCATGAAAACAAAAGAGCCG
  GTTAAAAAAATTTTTGTTGGTGGCCTTTCTCCAGATACACCTGAAGAGAAAATAAGGGAG
  TACTTTGGTGGTTTTGGTGAGGTGGAATCCATAGAGCTCCCCATGGACAACAAGACCAAT
  AAGAGGCGTGGGTTCTGCTTTATTACCTTTAAGGAAGAAGAACCAGTGAAGAAGATAATG
  GAAAAGAAATACCACAATGTTGGTCTTAGTAAATGTGAAATAAAAGTAGCCATGTCGAAG
  GAACAATATCAGCAACAGCAACAGTGGGGATCTAGAGGAGGATTTGCAGGAAGAGCTCGT
  GGAAGAGGTGGTGGCCCCAGTCAAAACTGGAACCAGGGATATAGTAACTATTGGAATCAA
  GGCTATGGCAACTATGGATATAACAGCCAAGGTTACGGTGGTTATGGAGGATATGACTAC
  ACTGGTTACAACAACTACTATGGATATGGTGATTATAGCAACCAGCAGAGTGGTTATGGG
  AAGGTATCCAGGCGAGGTGGTCATCAAAATAGCTACAAACCATACTAA
  
  Clone variation with respect to NM_031370.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031370 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCGGCGGCGCCAT
  TAAAGCGAGGAGGAGGCGAGAGCGGCCGCCGCTGGTGCTTATTCTTTTTTAGTGCAGCGG
  GAGAGAGCGGGAGTGTGCGCCGCGCGAGAGTGGGAGGCGAAGGGGGCAGGCCAGGGAGAG
  GCGCAGGAGCCTTTGCAGCCACGCGCGCGCCTTCCCTGTCTTGTGTGCTTCGCGAGGTAG
  AGCGGGCGCGCGGCAGCGGCGGGGATTACTTTGCTGCTAGTTTCGGTTCGCGGCAGCGGC
  GGGTGTAGTCTCGGCGGCAGCGGCGGAGACACTAGCACTATGTCGGAGGAGCAGTTCGGC
  GGGGACGGGGCGGCGGCAGCGGCAACGGCGGCGGTAGGCGGCTCGGCGGGCGAGCAGGAG
  GGAGCCATGGTGGCGGCGACACAGGGGGCAGCGGCGGCGGCGGGAAGCGGAGCCGGGACC
  GGGGGCGGAACCGCGTCTGGAGGCACCGATAGGGGCAGCGCCGAGTCGGAGGGGGCGAAG
  ATTGACGCCAGTAAGAACGAGGAGGATGAAGGCCATTCAAACTCCTCCCCACGACACTCT
  GAAGCAGCGACGGCACAGCGGGAAGAATGGAAAATGTTTATAGGAGGCCTTAGCTGGGAC
  ACTACAAAGAAAGATCTGAAGGACTACTTTTCCANATTTGGTGAAGTTGTAAACTGCACT
  CCTGAGTTAGATCCTATCACAGGGCGATCAAGGGGGTTTGGGCTTTGTGCTATTTAAAGA
  ATCGGAGAGTGTAAATAAGGTCATGGATCCAAAAGACATAAATTGATGGGAAGGTGATTG
  ATCCTAAAGGCCCAAAGCCATGAAACAAAANACCCGTTTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031370 unedited
  TGGCTATATTACAAAACANTAAGNAACTACTACATCATGACACTGTCACACTGGNGCTTT
  TAACACAAGACTTGCTCTACAATACTGGGGGAAAGGGCATAAAACACAAATTGATTCTGA
  AGCATAGCAATTAAGAAATAAAACAATGAAAGCAAATTTCTTTTAATGAGAACTCAGAAT
  TAAACTTCAGAGGGACCCAACGTCATACTTCCATTCAGGGACTTGATACAAAAAATTTAG
  TTTGAACTGCTATTAGCAGGTGGCAGGAGCCACCTTCAAATGAATCTTCAAATTGGAAAA
  TACTGCTTCACCACCTGTTGGGGATAAGTTGCAAATGGAATAATTTAGTATGGTTTGTAG
  CTATTTTGATGACCACCTCGCCTGGATACCTTCCCATAACCACTCTGCTGGTTGCTATAA
  TCACCATATCCATAGTAGTTGTTGTAACCAGTGTAGTCATATCCTCCATAACCACCGTAA
  CCTTGGCTGTTATATCCATAGTTGCCATAGCCTTGATTCCAATAGTTACTATATCCCTGG
  TTCCAGTTTTGACTGGGGCCACCACCTCTTCCACGAGCTCTTCCTGCAAATCCTCCTCTA
  GATCCCCACTGTTGCTGTTGCTGATATTGTTCCTTCGACATGGCTACTTTTATTTCACAT
  TTACTAAGACCAACATTGTGGTATTTCTTTTCCATTATCTTCTTCACTGGTTCTTCTTCC
  TTAAAGTAATAAAGCAGAACCCACGCCTCTTATTGGTCTTGTTGTCCATGGGGAGCTCTA
  TGGATTCCACCTCACCAAAACCACCAAAGTACTCCCTTATTTTCTCTTCAGGTGTATCTG
  GAGAAAGCCACCAACANAATTTTTTTAACCGCTCTTTTGTTTCATGGCTTTGGCCTTTTA
  GATCATCACCTTCCATTCATTNATGTCTTTTGACCATGACCTTACTACCTCTNCGATCTT
  TAATGCACAAGNCAAACCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031370
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031370.2, NP_112738.1
• RefSeq Size: 2257 bp
• RefSeq ORF: 1068 bp
• Locus ID: 3184
• UniProt ID: Q14103
• Cytogenetics: 4q21.22
• Domains: RRM
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a), also known as p45.