HNRNPD Rabbit Polyclonal Antibody

CAT#: TA377164

HNRNPD Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using HNRNPD antibody (TA377164) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of C6 cells using HNRNPD antibody (TA377164) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (TA377164) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using HNRNPD antibody (TA377164) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (TA377164) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HNRNPD antibody (TA377164). Western blot was performed from the immunoprecipitate using HNRNPD antibody (TA377164) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 30kDa/32kDa/36kDa/38kDa
• Gene Name: heterogeneous nuclear ribonucleoprotein D
• Database Link:
  Entrez Gene 3184 Human
  UniProt ID Q14103
• Background: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
• Synonyms: AUF1; AUF1A; hnRNPD0; HNRPD; P37