RAB27A (NM_183234) Human Untagged Clone

SKU SC107628

RAB27A (untagged)-Human RAB27A, member RAS oncogene family (RAB27A), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAB27A
• Synonyms: GS2; HsT18676; RAB27; RAM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107628 sequence for NM_183234 edited (data generated by NextGen Sequencing); ATGTCTGATGGAGATTATGATTACCTCATCAAGTTTTTAGCTTTGGGAGACTCTGGTGTA; GGGAAGACCAGTGTACTTTACCAATATACAGATGGTAAATTTAACTCCAAATTTATCACA; ACAGTGGGCATTGATTTCAGGGAAAAAAGAGTGGTGTACAGAGCCAGTGGGCCGGATGGA; GCCACTGGCAGAGGCCAGAGAATCCACCTGCAGTTATGGGACACAGCAGGGCAGGAGAGG; TTTCGTAGCTTAACGACAGCGTTCTTCAGAGATGCTATGGGTTTTCTTCTACTTTTTGAT; CTGACAAATGAGCAAAGTTTCCTCAATGTCAGAAACTGGATAAGCCAGCTACAGATGCAT; GCATATTGTGAAAACCCAGATATAGTGCTGTGTGGAAACAAGAGTGATCTGGAGGACCAG; AGAGTAGTGAAAGAGGAGGAAGCCATAGCACTCGCAGAGAAATATGGAATCCCCTACTTT; GAAACTAGTGCTGCCAATGGGACAAACATAAGCCAAGCAATTGAGATGCTTCTGGACCTG; ATAATGAAGCGAATGGAACGGTGTGTGGACAAGTCCTGGATTCCTGAAGGAGTGGTGCGA; TCAAATGGTCATGCCTCTACGGATCAGTTAAGTGAAGAAAAGGAGAAAGGGGCATGTGGC; TGTTGA; ; Clone variation with respect to NM_183234.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183234 unedited; GGGTTAGATTTTGTATCCGACTTACTATAGGGCGGCCGCGCAATTCGCACCAGGGTGGAC; GCGGCGCGCGAGGACCCGAGCCAGGCGGGGACAGGACGGCGGGGCGAGCCAGGTGAACTA; CTGAGTTCTTCATTATGTCTGATGGAGATTATGATTACCTCATCAAGTTTTTAGCTTTGG; GAGACTCTGGTGTAGGGAAGACCAGTGTACTTTACCAATATACAGATGGTAAATTTAACT; CCAAATTTATCACAACAGTGGGCATTGATTTCAGGGAAAAAAGAGTGGTGTACAGAGCCA; GTGGGCCGGATGGAGCCACTGGCAGAGGCCAGAGAATCCACCTGCAGTTATGGGACACAG; CAGGGCAGGAGAGGTTTCGTAGCTTAACGACAGCGTTCTTCAGAGATGCTATGGGTTTTC; TTCTACTTTTTGATCTGACAAATGAGCAAAGTTTCCTCAATGTCAGAAACTGGATAAGCC; AGCTACAGATGCATGCATATTGTGAAAACCCAGATATAGTGCTGTGTGGAAACAAGAGTG; ATCTGGAGGACCAGAGAGTAGTGAAAGAGGAGGAAGCCATAGCACTCGCAGAGAAATATG; GAATCCCCTACTTGAAACTAGTGCTGCCAATGGGACAAACATAAGCCAAGCAATTGAGAT; GCTTCTGGACCTGATAATGAAGCGAATGGAACGGTGTGTGGACAAGTCCTGGATTCCTGA; AGGAGTGGTGCGATCAAATGGTCATGCCTCTACGGATCAGTTAAGTGAAGAAAAGGAGAA; GGGGCATGTGGCTGTTGAGAAAGTCAAGTAGCGACATAGTAGTTCAGGTGGCCCCATGCC; TGGATCTTCTCTATGATTGATACATGGCACAGTGAGAGATTAATGGGCATTGTGTACCNA; ATGCTTCTCACCATCCCCATAGACCTANCGATAAGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_183234
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_183234.1, NP_899057.1
• RefSeq Size: 2812 bp
• RefSeq ORF: 666 bp
• Locus ID: 5873
• UniProt ID: P51159
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.