Ceramide synthase 2 (CERS2) (NM_022075) Human Untagged Clone

SKU SC107203

CERS2 (untagged)-Human LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Ceramide synthase 2
• Synonyms: L3; LASS2; SP260; TMSG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107203 sequence for NM_022075 edited (data generated by NextGen Sequencing); ATGCTCCAGACCTTGTATGATTACTTCTGGTGGGAACGTCTGTGGCTGCCTGTGAACTTG; ACCTGGGCCGATCTAGAAGACCGAGATGGACGTGTCTACGCCAAAGCCTCAGATCTCTAT; ATCACGCTGCCCCTGGCCTTGCTCTTCCTCATCGTTCGATACTTCTTTGAGCTGTACGTG; GCTACACCACTGGCTGCCCTCTTGAACATAAAGGAGAAAACTCGGCTGCGGGCACCTCCC; AACGCCACCTTGGAACATTTCTACCTGACCAGTGGCAAGCAGCCCAAGCAGGTGGAAGTA; GAGCTTTTGTCCCGGCAGAGCGGGCTCTCTGGCCGCCAGGTAGAGCGTTGGTTCCGTCGC; CGCCGCAACCAGGACCGGCCCAGTCTCCTCAAGAAGTTCCGAGAAGCCAGCTGGAGATTC; ACATTTTACCTGATTGCCTTCATTGCCGGCATGGCCGTCATTGTGGATAAACCCTGGTTC; TATGACATGAAGAAAGTTTGGGAGGGATATCCCATACAGAGCACTATCCCTTCCCAGTAT; TGGTACTACATGATTGAACTTTCCTTCTACTGGTCCCTGCTCTTCAGCATTGCCTCTGAT; GTCAAGCGAAAGGATTTCAAGGAACAGATCATCCACCATGTGGCCACCATCATTCTCATC; AGCTTTTCCTGGTTTGCCAATTACATCCGAGCTGGGACTCTAATCATGGCTCTGCATGAC; TCTTCCGATTACCTGCTGGAGTCAGCCAAGATGTTTAACTACGCGGGATGGAAGAACACC; TGCAACAACATCTTCATCGTCTTCGCCATTGTTTTTATCATCACCCGACTGGTCATCCTG; CCCTTCTGGATCCTGCATTGCACCCTGGTGTACCCACTGGAGCTCTATCCTGCCTTCTTT; GGCTATTACTTCTTCAATTCCATGATGGGAGTTCTACAGCTGCTGCATATCTTCTGGGCC; TACCTCATTTTGCGCATGGCCCACAAGTTCATAACTGGAAAGCTGGTAGAAGATGAACGC; AGTGACCGGGAAGAAACAGAGAGCTCAGAGGGGGAGGAGGCTGCAGCTGGGGGAGGAGCA; AAGAGCCGGCCCCTAGCCAATGGCCACCCCATCCTCAATAACAACCATCGTAAGAATGAC; TGA; ; Clone variation with respect to NM_022075.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022075 unedited; NGGGCCTGTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACCAGGCAG; CGGCCCCGGCCCCGCCACGCTGCCGCCGGGATGCTCCAGACCTTGTATGATTACTTCTGG; TGGGAACGTCTGTGGCTGCCTGTGAACTTGACCTGGGCCGATCTAGAAGACCGAGATGGA; CGTGTCTACGCCAAAGCCTCAGATCTCTATATCACGCTGCCCCTGGCCTTGCTCTTCCTC; ATCGTTCGATACTTCTTTGAGCTGTACGTGGCTACACCACTGGCTGCCCTCTTGAACATA; AAGGAGAAAACTCGGCTGCGGGCACCTCCCAACGCCACCTTGGAACATTTCTACCTGACC; AGTGGCAAGCAGCCCAAGCAGGTGGAAGTAGAGCTTTTGTCCCGGCAGAGCGGGCTCTCT; GGCCGCCAGGTAGAGCGTTGGTTCCGTCGCCGCCGCAACCAGGACCGGCCCAGTCTCCTC; AAGAAGTTCCGAGAAGCCAGCTGGAGATTCACATTTTACCTGATTGCCTTCATTGCCGGC; ATGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGAAGAAAGTTTGGGAGGGATAT; CCCATACAGAGCACTATCCCTTCCCAGTATTGGTACTACATGATTGAACTTTCCTTCTAC; TGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAGGATTTCAAGGAACAGATC; ATCCACCATGTGGCCACCATCATTCTCATCAGCTNTTCCTGGTTTGCCAATTACATCCGA; GCTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAG; ATGTTTAACTACGCGGGATGGAAGAACACCTGNCACACATCTTCATCGTCTTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022075 unedited; NGTACCGGTATGTTCACACTTACTCTGAGCAGNANAGCACTGGGGNAGGGTCACAGGGAT; GCCACCCGGGATCTGTTCAGGAAACAGCTGTGACCGCGGCCGCAATCTAAAGTCGAGTTT; TTGCGTTTTCTTTTTCTTTTTTTATTTAATATTTTTTATTTAATCTTTTAATTTTAAAAA; AAAACCCATTAACAGTACATTTTGCTCTAAAATGGGCCCTCTGCTGAAATGCTAGGCGGC; TCGGCCGGAATTCTGGGTTTAACCCCCCAGCCTCTATCTTTNAGGGATAAGAATTAGAAT; TAGTTGCGATTCTGCTCGACGCCGGTCTCGCTCAGCCTGGCAGCAGCCGGAAGGGACCCC; ACCATCCACTCCCATCGCAGGAGCGTCCTAATAGACTGGAAATCCGGGGCAGACAGCCCC; GTGCGACGCGCCGCTCCGCGCGCAACGAGGCCTCCCACGCCCGGCGTGACAATCCCGTCC; GCAACCTCCGCCCGTGACCCGCCCTCCGCCACTGCGACCCCGCTTAATACTGCAGTCGCC; AGCCAACGCCGCCCGAGCGACGCAGCCGGTCCGGACGAGACTGTCCCCCGCAGCGGGCTA; AGCAATGTCTCAGCAAAACCACGCACAGGTGCTACTCCGCCGCCTACAGCAGGGAGGCGC; CTACAGTCGCCCCAAACCTCTCGCCCCACCATGCGCCGTTATGGCCACTCGCACTCGCTC; CGCCGCTCCGCCAGCGCCTACTCATACCACCGCCGCACGCCGACAGATCCGGCCCGCAGG; CCCCAAGCCACTGACGCCAGCGGACAGCAGAACCGCGGAGCGATCGATCACCCAGCCGGC; GTCTTCCGCCACACTCACCGCATCGCGTCCGGCACGCCATTCTTCGCTCGTCTCTGATTT; ACGCGTCCGCTCCATTCGATGCCTGCGGGTGTCCGGTCCTGCCCGCTCCTGCTCGGCGCC; CGCCACGCCTCCAACGCTGGCTTCACGGTGCTCATCCAAACTGCGAGGGCGTGACTACGC; GCGTGGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_022075
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022075.1, NP_071358.1
• RefSeq Size: 2250 bp
• RefSeq ORF: 1143 bp
• Locus ID: 29956
• UniProt ID: Q96G23
• Cytogenetics: 1q21.3
• Protein Families: Transcription Factors, Transmembrane
• Summary: This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.