GPAM (NM_020918) Human Untagged Clone

CAT#: SC106982

GPAM (untagged)-Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPAM
• Synonyms: GPAT; GPAT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_020918 edited
  GAATTCGGCACGAGGAGTGCGGGCTTCTGCAGCAGCCGAAGCTGGAGCTGCTAGGGCAGC
  AGCGGCTCCCCTGTTGTATGGACATTCTGCACCCGAAACTGATAGCTGAGTCCTGAAGTT
  TTATGTTATGAAACAGAAGAACTTTCATCCCAGCACATGATTTGGGAATTACACTTTGTG
  ACATGGATGAATCTGCACTGACCCTTGGTACAATAGATGTTTCTTATCTGCCACATTCAT
  CAGAATACAGTGTTGGTCGATGTAAGCACACAAGTGAGGAATGGGGTGAGTGTGGCTTTA
  GACCCACCGTCTTCAGATCTGCAACTTTAAAATGGAAAGAAAGCCTAATGAGTCGGAAAA
  GGCCATTTGTTGGAAGATGTTGTTACTCCTGCACTCCCCAGAGCTGGGACAAATTTTTCA
  ACCCCAGTATCCCGTCTTTGGGTTTGCGGAATGTTATTTATATCAATGAAACTCACACAA
  GACACCGCGGATGGCTTGCAAGACGCCTTTCTTACGTTCTTTTTATTCAAGAGCGAGATG
  TGCATAAGGGCATGTTTGCCACCAATGTGACTGAAAATGTGCTGAACAGCAGTAGAGTAC
  AAGAGGCAATTGCAGAAGTGGCTGCTGAATTAAACCCTGATGGTTCTGCCCAGCAGCAAT
  CAAAAGCCGTTAACAAAGTGAAAAAGAAAGCTAAAAGGATTCTTCAAGAAATGGTTGCCA
  CTGTCTCACCGGCAATGATCAGACTGACTGGGTGGGTGCTGCTAAAACTGTTCAACAGCT
  TCTTTTGGAACATTCAAATTCACAAAGGTCAACTTGAGATGGTTAAAGCTGCAACTGAGA
  CGAATTTGCCGCTTCTGTTTCTACCAGTTCATAGATCCCATATTGACTATCTGCTGCTCA
  CTTTCATTCTCTTCTGCCATAACATCAAAGCACCATACATTGCTTCAGGCAATAATCTCA
  ACATCCCAATCTTCAGTACCTTGATCCATAAGCTTGGGGGCTTCTTCATACGACGAAGGC
  TCGATGAAACACCAGATGGACGGAAAGATGTTCTCTATAGAGCTTTGCTCCATGGGCATA
  TAGTTGAATTACTTCGACAGCAGCAATTCTTGGAGATCTTCCTGGAAGGCACACGTTCTA
  GGAGTGGAAAAACCTCTTGTGCTCGGGCAGGACTTTTGTCAGTTGTGGTAGATACTCTGT
  CTACCAATGTCATCCCAGACATCTTGATAATACCTGTTGGAATCTCCTATGATCGCATTA
  TCGAAGGTCACTACAATGGTGAACAACTGGGCAAACCTAAGAAGAATGAGAGCCTGTGGA
  GTGTAGCAAGAGGTGTTATTAGAATGTTACGAAAAAACTATGGTTGTGTCCGAGTGGATT
  TTGCACAGCCATTTTCCTTAAAGGAATATTTAGAAAGCCAAAGTCAGAAACCGGTGTCTG
  CTCTACTTTCCCTGGAGCAAGCGTTGTTACCAGCTATACTTCCTTCAAGACCCAGTGATG
  CTGCTGATGAAGGTAGAGACACGTCCATTAATGAGTCCAGAAATGCAACAGATGAATCCC
  TACGAAGGAGGTTGATTGCAAATCTGGCTGAGCATATTCTATTCACTGCTAGCAAGTCCT
  GTGCCATTATGTCCACACACATTGTGGCTTGCCTGCTCCTCTACAGACACAGGCAGGGAA
  TTGATCTCTCCACATTGGTCGAAGACTTCTTTGTGATGAAAGAGGAAGTCCTGGCTCGTG
  ATTTTGACCTGGGGTTCTCAGGAAATTCAGAAGATGTAGTAATGCATGCCATACAGCTGC
  TGGGAAATTGTGTCACAATCACCCACACTAGCAGGAACGATGAGTTTTTTATCACCCCCA
  GCACAACTGTCCCATCAGTCTTCGAACTCAACTTCTACAGCAATGGGGTACTTCATGTCT
  TTATCATGGAGGCCATCATAGCTTGCAGCCTTTATGCAGTTCTGAACAAGAGGGGACTGG
  GGGGTCCCACTAGCACCCCACCTAACCTGATCAGCCAGGAGCAGCTGGTGCGGAAGGCGG
  CCAGCCTGTGCTACCTTCTCTCCAATGAAGGCACCATCTCACTGCCTTGCCAGACATTTT
  ACCAAGTCTGCCATGAAACAGTAGGAAAGTTTATCCAGTATGGCATTCTTACAGTGGCAG
  AGCACGATGACCAGGAAGATATCAGTCCTAGTCTTGCTGAGCAGCAGTGGGACAAGAAGC
  TTCCTGAACCTTTGTCTTGGAGAAGTGATGAAGAAGATGAAGACAGTGACTTTGGGGAGG
  AACAGCGAGATTGCTACCTGAAGGTGAGCCAATCCAAGGAGCACCAGCAGTTTATCACCT
  TCTTACAGAGACTCCTTGGGCCTTTGCTGGAGGCCTACAGCTCTGCTGCCATCTTTGTTC
  ACAACTTCAGTGGTCCTGTTCCAGAACCTGAGTATCTGCAAAAGTTGCACAAATACCTAA
  TAACCAGAACAGAAAGAAATGTTGCAGTATATGCTGAGAGTGCCACATATTGTCTTGTGA
  AGAATGCTGTGAAAATGTTTAAGGATATTGGGGTTTTCAAGGAGACCAAACAAAAGAGAG
  TGTCTGTTTTAGAACTGAGCAGCACTTTTCTACCTCAATGCAACCGACAAAAACTTCTAG
  AATATATTCTGAGTTTTGTGGTGCTGTAGGTAACGTGTGGCACTGCTGGCAAATGAAGGT
  CATGAGATGAGTTCCTTGTAGGTACCAGCTTCTGGCTCAAGAGTTGAAGGTGCCGTCGCA
  GGGTCAGGCCTGCCCTGTCCCGAGGTGATCTCCTGGAAGACAAGTGCCTTCTCCCTCCAT
  GGATCTGTGATCTTCCCAGCTCTGCATCAACACAGCAGCCTGCAGATAACACTTGGGGGG
  ACCTCAGCCTCTATTCGCAACTCATAATCCGTAGACTACAAGATGAAATCTCAATAAATT
  ATTTTTGAGTTTATTAAAGATTGACATTTTAAGTACAACGTTTAAGGACTAATTACTGTG
  ATGGACACAGAAATGTAGCTGTGTTCTGGAACTG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020918 unedited
  ATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAGTGCGGGCTTC
  TGCAGCAGCCGAAGCTGGAGCTGCTAGGGCAGCAGCGGCTCCCCTGTTGTATGGACATTC
  TGCACCCGAAACTGATAGCTGAGTCCTGAAGTTTTATGTTATGAAACAGAAGAACTTTCA
  TCCCAGCACATGATTTGGGAATTACACTTTGTGACATGGATGAATCTGCACTGACCCTTG
  GTACAATAGATGTTTCTTATCTGCCACATTCATCAGAATACAGTGTTGGTCGATGTAAGC
  ACACAAGTGAGGAATGGGGTGAGTGTGGCTTTAGACCCACCGTCTTCAGATCTGCAACTT
  TAAAATGGAAAGAAAGCCTAATGAGTCGGAAAAGGCCATTTGTTGGAAGATGTTGTTACT
  CCTGCACTCCCCAGAGCTGGGACAAATTTTTCAACCCCAGTATCCCGTCTTTGGGTTTGC
  GGAATGTTATTTATATCAATGAAACTCACACAAGACACCGCGGATGGCTTGCAAGACGCC
  TTTCTTACGTTCTTTTTATTCAAGAGCGAGATGTGCATAAGGGCATGTTTGCCACCAATG
  TGACTGAAAATGTGCTGAACAGCAGTAGAGTACAAGAGGCAATTGCAGAAGTGGCTGCTG
  AATTAAACCCTGATGGTTCTGCCCAGCAGCAATCAAAAGCCGTTAACAAAGTGAAAAAGA
  AAGCTAAAAGGATTCTTCAAGAAATGGTTGCCACTGTCTCACCGGGCATGATCAGACTGA
  CTGGGTGGGTGCTGCTAAAACTGTTCAACAGCTTCTTTTGGACATCAAATTCACAAAGGT
  CAAACTTGAGATGTTAAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020918 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACTTGACCACAGTCTT
  CCCCTGAAGAAGGGCATGCATTTCATGTTACAGAAATGCGATAGAGTAAACTGTAATAAA
  TTATTTACAAGCACCTAGTTTGTTTAACCTTATGTGGAAGCCATCACTGTTGGAAAACAA
  TGAGAATGTATCTTTTAGCAAAACGGTGCTATCTGGAAGCTTCATTGTGAAGATGCTTTC
  GTTTTTTTGTTTTTTGTTTTCAGTCATGAGGCACTGAAGAATGAGTTGTGCTGAAATTAA
  CTCAAAGGTCAGCTTGTCTGGATGAGCATAACTTTGGTTGAGATTTTTCTCCCTTAAAAA
  AGATTTTCAAAGCACCAGTTAATTACAAAAAGCATGCATATTTATCCTCACAGTGAGTTA
  AGTGGTGAGAGAGCTAGCAAATCATACATTGCATTCCCCAAAGCATCTGAACGTACTTCT
  AGAAAACAAACCAACCAAAAGGGAAAATAATGCAAGAGAAGCCGTATTTTCTTTGCTTAG
  GTTGGCAAAGCAGCAGCTCTTTGCAGCAATGACAGGCAGGGCAGAGTGTCGACTGGGAAG
  CGAGTCCCAATCTTGAATAATGGTGAGCTTGAGTAATCCTTAATTTACAATTTAAAGGAT
  GAAAAAAAGAGACTAGTGAAATGTTTGCTTCCCCAGATAGGAACACCCAAGACTCCCTGG
  GTATCCTCCAAATGTAGCCAAGAAAAGTGNTCTTCTACCCTAGTCAGTAAAATGGGATGC
  TAAAACATGATATCTCATTCACATCAGAGCTACTACAACTGACAATGACTTCATGGGATA
  CATCAATTCCTATAAGAAAAAATATATTNTAAATGCAGAACTTGAGAGGGATCACACAGC
  ATAGCATTATTGCTATGAGTTTCAAACATGGGATTTCATACAATGTGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020918
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020918.2, NP_065969.2
• RefSeq Size: 6387 bp
• RefSeq ORF: 2487 bp
• Locus ID: 57678
• UniProt ID: Q9HCL2
• Cytogenetics: 10q25.2
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.