CHMP4B (NM_176812) Human Untagged Clone

CAT#: SC106955

CHMP4B (untagged)-Human chromatin modifying protein 4B (CHMP4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHMP4B
• Synonyms: C20orf178; CHMP4A; CTPP3; CTRCT31; dJ553F4.4; Shax1; SNF7; SNF7-2; Vps32-2; VPS32B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106955 sequence for NM_176812 edited (data generated by NextGen Sequencing)
  ATGTCGGTGTTCGGGAAGCTGTTCGGGGCTGGAGGGGGTAAGGCCGGCAAGGGCGGCCCG
  ACCCCCCAGGAGGCCATCCAGCGGCTGCGGGACACGGAAGAGATGTTAAGCAAGAAACAG
  GAGTTCCTGGAGAAGAAAATCGAGCAGGAGCTGACGGCCGCCAAGAAGCACGGCACCAAA
  AACAAGCGCGCGGCCCTCCAGGCACTGAAGCGTAAGAAGAGGTATGAGAAGCAGCTGGCG
  CAGATCGACGGCACATTATCAACCATCGAGTTCCAGCGGGAGGCCCTGGAGAATGCCAAC
  ACCAACACCGAGGTGCTCAAGAACATGGGCTATGCCGCCAAGGCCATGAAGGCGGCCCAT
  GACAACATGGACATCGATAAAGTTGATGAGTTAATGCAGGACATTGCTGACCAGCAAGAA
  CTTGCAGAGGAGATTTCAACAGCAATTTCGAAACCTGTAGGGTTTGGAGAAGAGTTTGAC
  GAGGATGAGCTCATGGCGGAATTAGAAGAACTAGAACAGGAGGAACTAGACAAGAATTTG
  CTGGAAATCAGTGGACCCGAAACAGTCCCTCTACCAAATGTTCCCTCTATAGCCCTACCA
  TCAAAACCCGCCAAGAAGAAAGAAGAGGAGGACGACGACATGAAGGAATTGGAGAACTGG
  GCTGGATCCATGTAA
  
  Clone variation with respect to NM_176812.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176812 unedited
  CGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCGGAGGAGAGGCCTGCGGCGGCAG
  GGAGCGGCGGGACTGGGAGCGGGCGCCGGAGCCGACCCGAGCCGAGCCGAGCCGAGCCGA
  GCCGGAGCGGGCGGCGAAGGCCGGCGCGGCGAGCAGCAACCATGTCGGTGTTCGGGAAGC
  TGTTCGGGCTGGAGGGGTAAAGGCCGGCAAGGGCGGCCCGACCCCCCAGGAGGCCATCCA
  GCGGCTGCGGGACACGGAAGAGATGTTAAGCAAGAAACAGGAGTTCCTGGAGAAGAAAAT
  CGAGCAGGAGCTGACGGCCGCCAAGAAGCACGGCACCAAAAACAAGCGCGCGGCCCTCCA
  GGCACTGAAGCGTAAGAAGAGGTATGAGAAGCAGCTGGCGCAGATCGACGGCACATTATC
  AACCATCGAGTTCCAGCGGGAGGCCCTGGAGAATGCCAACACCAACACCGAGGTGCTCAA
  GAACATGGGCTATGCCGCCAAGGCCATGAAGGCGGCCCATGACAACATGGACATCGATAA
  AGTTGATGAGTTAATGCAGGACATTGCTGACCAGCAAGAACTTGCNAGAGGAGATTTCAA
  CAGCAATTTCGAACCTGTNAGGGGTTTGGAGAAAAGAGTTTGACGAAGGATGAAGCTCAT
  GGCNNNGGATTAGAAGAACTAGAACAGGGAGGAACTAGACAAGAATTTGCTGGAAATCAG
  TGGACCCGAAACAGTCCCTCTACCANATGTTNCCTCTATAGCCCTACCATCAAACCCGNC
  AGAGAANNGAGNAGGAGGACGACACNTGAAGGANTGGNAGACTTGGNGCTGATCATGTNA
  AAGGGGGCCCAGCGCTGGCTGGCCAAACGACAGTGGGGTGGCTGGCACGAGCAGCGTTGC
  CTTTGGGGGGCAGCACAAATGGTGCCAAACAGGTCCTCGTTTCACTCTCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176812
• Insert Size: 1550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176812.3, NP_789782.1
• RefSeq Size: 1685 bp
• RefSeq ORF: 675 bp
• Locus ID: 128866
• UniProt ID: Q9H444
• Cytogenetics: 20q11.22
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.[provided by RefSeq, Oct 2009]