NSUN2 (NM_017755) Human Untagged Clone

CAT#: SC106395

NSUN2 (untagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NSUN2
• Synonyms: MISU; MRT5; SAKI; TRM4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106395 sequence for NM_017755 edited (data generated by NextGen Sequencing)
  ATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGCCGGAGGACGCGGAG
  GATGGCGCCGAGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAGGAGGCTACCCCGAG
  ATCGTCAAGGAGAACAAGCTGTTCGAGCACTACTACCAGGAGCTCAAGATCGTGCCCGAG
  GGCGAGTGGGGCCAGTTCATGGACGCTCTCAGGGAGCCGCTCCCGGCCACTTTAAGAATT
  ACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCTTAAAGAACAAATATTTTAAG
  GAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCACAGCCACTGAGTTGG
  TATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCTTGAGAAAATCGCCA
  CACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTGGAAATATTAGTCGT
  CAAGAAGCTGTTAGCATGATCCCACCACTGCTCCTCAACGTGCGGCCTCATCATAAGATC
  TTAGATATGTGTGCAGCACCTGGCTCAAAGACCACACAGTTAATTGAAATGCTACATGCC
  GACATGAATGTCCCCTTTCCAGAGGGATTTGTTATTGCGAATGATGTGGACAACAAGCGC
  TGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCCCCTGCATCATGGTGGTCAAC
  CATGATGCCTCCAGCATACCCAGGCTCCAGATAGATGTGGACGGCAGGAAAGAGATCCTC
  TTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGAGACGGCACTATGAGAAAAAAC
  ATTGATGTTTGGAAAAAGTGGACCACCTTAAATAGCTTGCAGCTACATGGCTTACAGCTG
  CGGATTGCAACACGCGGGGCTGAACAGCTGGCTGAAGGTGGAAGGATGGTGTATTCCACG
  TGTTCACTAAACCCTATTGAGGATGAAGCAGTCATAGCATCTTTACTGGAAAAAAGTGAA
  GGTGCTTTGGAGCTTGCTGATGTGTCTAATGAACTGCCAGGGCTGAAGTGGATGCCTGGA
  ATCACACAGTGGAAGGTAATGACGAAAGATGGGCAGTGGTTTACAGACTGGGACGCTGTT
  CCTCACAGCAGACACACCCAGATCCGACCTACCATGTTCCCTCCGAAGGACCCAGAAAAG
  CTGCAGGCCATGCACCTGGAGCGATGCCTTAGGATATTACCCCATCATCAGAATACTGGA
  GGGTTTTTTGTGGCAGTATTGGTGAAAAAATCTTCAATGCCGTGGAATAAACGTCAGCCA
  AAGCTTCAGGGTAAATCTGCAGAGACCAGAGAAAGCACACAGCTGAGCCCTGCAGATCTC
  ACAGAAGGGAAACCCACAGATCCCTCTAAGCTGGAAAGTCCGTCATTCACAGGAACTGGT
  GACACAGAAATAGCTCATGCAACTGAGGATTTAGAGAATAATGGCAGTAAGAAAGATGGC
  GTGTGTGGTCCTCCTCCATCAAAGAAAATGAAGTTATTTGGATTTAAAGAAGATCCATTT
  GTATTTATTCCTGAAGATGACCCATTATTTCCACCTATTGAGAAATTTTATGCTTTGGAT
  CCTTCATTCCCAAGGATGAATTTGTTAACTCGGACTACAGAAGGGAAGAAAAGGCAGCTC
  TACATGGTTTCTAAGGAGTTGCGGAATGTGCTGCTGAATAACAGTGAGAAGATGAAGGTT
  ATTAACACAGGGATCAAAGTCTGGTGTAGAAATAACAGCGGTGAAGAGTTTGACTGTGCT
  TTCCGGCTGGCACAGGAGGGAATATATACATTGTATCCATTTATTAACTCAAGAATTATT
  ACTGTATCAATGGAAGATGTTAAGATACTGTTGACCCAGGAAAATCCCTTTTTTAGAAAA
  CTCAGCAGTGAGACCTACAGTCAAGCAAAGGACCTGGCAAAGGGAAGCATCGTGCTGAAG
  TATGAACCAGATTCTGCGAATCCAGACGCTCTGCAGTGTCCCATCGTCTTATGCGGATGG
  CGGGGAAAGGCCTCCATTCGAACTTTTGTGCCCAAGAATGAACGGCTTCATTATCTCAGG
  ATGATGGGGCTGGAGGTATTGGGAGAAAAGAAGAAGGAAGGGGTTATCCTCACAAATGAG
  AGTGCAGCCAGCACCGGACAGCCAGACAATGACGTGACTGAGGGACAGAGAGCAGGAGAG
  CCCAACAGCCCAGATGCAGAAGAGGCCAACAGTCCAGACGTGACAGCAGGCTGTGACCCG
  GCGGGGGTCCATCCACCCCGGTGA
  
  Clone variation with respect to NM_017755.5
  1749 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017755 unedited
  GGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGCCGGAAGTC
  CCGGGAGGTGTAGGGCTAGAGTTCTGGCCGTGGCGGGCCGGTTTCTGCGTGCTGCGTGCG
  CGGCCGCGTGGGCTATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGC
  CGGAGGACGCGGAGGATGGCGCCGAGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAG
  GAGGCTACCCCGAGATCGTCAAGGAGAACAAGCTGTTCGAGCACTACTACCAGGAGCTCA
  AGATCGTGCCCGAGGGCGAGTGGGGCCAGTTCATGGACGCTCTCAGGGAGCCGCTCCCGG
  CCACTTTAAGAATTACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCTTAAAGA
  ACAAATATTTTAAGGAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCAC
  AGCCACTGAGTTGGTATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCT
  TGAGAAAATCGCCACACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTG
  GAAATATTAGTCGTCAAGAAGCTGTTAGCATGATCCCACCACTGCTCCTCAACGTGCGGC
  CTCATCATAAGATCTTAGATATGTGTGCAGCACCTGGCTCAAGACCACACAGTTAATTGA
  AATGCTACATGCCGACATGAATGTCCCCTTTCCAGAGGGATTTGNTATTGCGAATGATGT
  GGACAACAGCGCTGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCCCCTGCATC
  ATGGTGGTCAACCATGATGCCTCCAGCATACCCCAGCTTCAGATAGATGTGGACGGGCAG
  AAAGAGATCCTCTNCTATGATCGAATTTATGTGATGTCCTTGCAGTGAGACGCACTATGA
  AAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017755 unedited
  NGGTTCGCCCATCGGNNGATGGCACTTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCAC
  AGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC
  GAGTTTTTTTTTTTTTTTTTTATATTTTATCAATTTTATTGAAATATTCCAAGGATCCCA
  ACCCCATTTAAAAATAAAAATTGTAAAGCACTCCATTCAATAAAAGCACATAAGTCCCCC
  TCAATAATTAGTATGACAATTCACGATACAGCTCTTACTCTGGGAGAGTTTATTTTACCC
  TTTATTCCAAAAGGCACAAAGTCATCTGAGGCCTCAGATATTAACCCCACTGCATGTTAA
  TGACACACCACTGAGGTGCAGCTCAATGTAATTATTAAAGCTTATAACACACTTCCCCAA
  GAATTTATAGATTCTTTCTATAAATAATAATTTAAAAAATACTGCACCTTAAGACCAATA
  CAGGCTTAACAAAAGACCTGAAATTTCTGCAAGGGCAGTTTTGTTTCTTGATAGAAGTAC
  AACTTTTGAAAGTCTATTCCCAGCAAAAGAAACACTAGACCCAGCTTGGCCAAAGAAACA
  AAATAAAACAAGTGATTTCTAACACGCTAAAAGAGTACATTTTCATCAGCTCCAAAGAAA
  GCAGTCCTGGTCATTCAGAAGGCTCCTATGATCCCACCAGTCTGCAGTCATTAGAAATAT
  ATGCTTTACAGGCCACAGGCTGCTCTGGATTTGGTTTCAGACACCAGTGACCAGAAGAAG
  CCAGTTTTGCGTGTGAGGGGGTGTGGGCCCCCGCTGCCTTGGGCCTGCTCACCGNGGTGG
  ATGGACCCCCGCCNGGTCACAGCCTGCTGTCACGTCTGGACTGTTGGCCTCTTCTGCATC
  TGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017755
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017755.4, NP_060225.4
• RefSeq Size: 3089 bp
• RefSeq ORF: 2304 bp
• Locus ID: 54888
• UniProt ID: Q08J23
• Cytogenetics: 5p15.31
• Domains: Nol1_Nop2_Sun
• Gene Summary: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1).