LRRC8C (NM_032270) Human Untagged Clone

CAT#: SC105781

LRRC8C (untagged)-Human leucine rich repeat containing 8 family, member C (LRRC8C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC8C
• Synonyms: AD158; FAD158
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105781 sequence for NM_032270 edited (data generated by NextGen Sequencing)
  ATGATTCCCGTGACAGAATTCCGGCAGTTCTCTGAGCAGCAGCCTGCCTTCCGAGTGCTG
  AAGCCATGGTGGGATGTGTTTACCGATTACCTCTCAGTAGCCATGCTCATGATCGGCGTG
  TTTGGATGTACTTTACAGGTCATGCAAGACAAGATAATCTGCCTTCCGAAAAGAGTGCAG
  CCTGCTCAGAACCACTCTTCCCTTTCGAATGTCTCTCAAGCAGTTGCCAGTACCACTCCA
  CTGCCTCCACCTAAACCATCTCCTGCTAACCCCATCACTGTGGAAATGAAAGGCCTGAAG
  ACAGATTTGGACCTTCAGCAGTACAGCTTTATAAATCAGATGTGTTATGAGCGAGCCCTC
  CACTGGTATGCCAAGTATTTCCCTTACCTTGTCCTCATCCATACCCTGGTCTTTATGCTC
  TGCAGTAACTTTTGGTTCAAATTCCCTGGTTCCAGCTCCAAAATAGAACATTTCATCTCC
  ATTCTGGGGAAGTGTTTTGACTCTCCTTGGACCACACGGGCTTTATCTGAAGTGTCTGGG
  GAGGACTCAGAAGAAAAGGACAACAGGAAGAACAACATGAACAGGTCCAACACCATCCAA
  TCTGGTCCAGAAGGCAGCCTGGTCAACTCTCAGTCTTTAAAGTCCATTCCTGAGAAGTTT
  GTAGTTGATAAATCCACTGCAGGGGCTCTGGATAAAAAGGAAGGTGAGCAGGCTAAGGCC
  TTATTTGAGAAGGTGAAGAAGTTCAGGCTGCATGTGGAAGAAGGTGATATTCTATATGCC
  ATGTATGTTCGCCAGACTGTACTTAAAGTTATCAAATTCCTAATCATCATTGCATATAAT
  AGTGCTCTGGTTTCCAAGGTCCAGTTTACAGTGGACTGTAATGTGGACATTCAGGACATG
  ACTGGATATAAAAACTTTTCTTGCAATCATACCATGGCACACTTGTTCTCAAAACTGTCC
  TTTTGCTATCTGTGCTTTGTTAGTATCTATGGATTGACGTGCCTTTATACCTTATACTGG
  CTGTTCTACCGTTCTCTACGGGAATATTCCTTTGAGTATGTCCGTCAGGAGACTGGAATT
  GATGATATTCCAGATGTGAAAAATGACTTTGCTTTTATGCTTCATATGATAGATCAGTAT
  GACCCTCTCTATTCCAAGAGATTTGCAGTGTTCCTGTCTGAAGTCAGTGAAAACAAATTA
  AAGCAGCTGAACTTAAATAACGAATGGACTCCTGATAAACTGAGGCAGAAGCTACAGACA
  AATGCCCATAATCGACTGGAATTGCCTCTTATCATGCTCTCTGGCCTTCCAGACACTGTT
  TTTGAAATCACAGAGTTGCAATCTCTAAAACTTGAAATCATTAAGAACGTAATGATACCA
  GCCACCATTGCACAGCTAGACAATCTTCAAGAGCTCTCTCTGCACCAGTGTTCTGTCAAA
  ATCCACAGTGCGGCGCTCTCTTTCCTGAAGGAAAACCTCAAGGTCTTGAGCGTCAAGTTT
  GATGACATGAGGGAACTCCCCCCCTGGATGTATGGGCTCCGAAATCTGGAAGAGCTGTAC
  CTAGTTGGCTCTCTAAGTCATGATATTTCCAGAAATGTCACCCTTGAGTCTCTGCGGGAT
  CTCAAAAGCCTTAAAATTCTCTCTATCAAAAGCAACGTTTCCAAAATCCCTCAGGCAGTG
  GTTGATGTTTCCAGCCATCTCCAGAAGATGTGCATACATAATGATGGCACCAAGCTGGTG
  ATGCTCAACAACTTAAAGAAGATGACCAATCTGACAGAGCTGGAGCTGGTCCACTGTGAC
  CTGGAGCGTATTCCTCATGCTGTGTTCAGCCTACTCAGCCTCCAGGAATTGGACCTGAAG
  GAAAACAATCTGAAATCTATAGAAGAAATCGTTAGCTTTCAGCACTTAAGAAAGTTGACA
  GTGCTAAAACTGTGGCATAACAGCATCACCTACATCCCAGAGCATATAAAGAAACTCACC
  AGCCTGGAACGCCTGTCCTTTAGTCACAATAAAATAGAGGTGCTGCCTTCCCACCTCTTC
  CTATGCAACAAGATCCGATACTTGGACTTATCGTACAATGACATTCGATTTATCCCCCCT
  GAAATTGGAGTTCTACAAAGTTTACAGTATTTTTCCATCACATGTAACAAAGTGGAAAGC
  CTTCCAGATGAACTCTACTTCTGCAAGAAACTTAAAACTCTGAAGATTGGAAAAAACAGC
  CTATCTGTACTTTCACCGAAAATTGGAAATTTGCTATTTCTTTCCTACTTAGATGTAAAA
  GGTAATCACTTTGAAATCCTCCCTCCTGAACTGGGTGACTGTCGGGCTCTGAAGCGAGCT
  GGTTTAGTTGTAGAAGATGCTCTGTTTGAAACTCTGCCTTCTGACGTCCGGGAGCAAATG
  AAAACAGAATAA
  
  Clone variation with respect to NM_032270.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032270 unedited
  TAAGTTCAGATTTGTATACGACTCATATAGGCGGCCGCGAATTCGCACGAGGGCTTGCGG
  GGCTGGGGCGTTAGCTGCGTCCCGGCTGGAAGCCCGCGAAGAGAAGTAGGAGGAAGGCGC
  CGCCGTGGCCGCGGCCGCAGTGCTCGGGCGCGACAAGCCATGAGCAGCGACCCCGCGGGC
  GACGCCGCCGTCCCTGGCAGCCGCTGAGCCCGACCCCCAGGCAGCTCGCCGCTCCCTAGC
  ACCTTCTCCAGTCGACACTCTCGCGCCCGGAGAAACATGATTCCCGTGACAGAATTCCGG
  CAGTTCTCTGAGCAGCAGCCTGCCTTCCGAGTGCTGAAGCCATGGTGGGATGTGTTTACC
  GATTACCTCTCAGTAGCCATGCTCATGATCGGCGTGTTTGGATGTACTTTACAGGTCATG
  CAAGACAAGATAATCTGCCTTCCGAAAAGAGTGCAGCCTGCTCAGAACCACTCTTCCCTT
  TCGAATGTCTCTCAAGCAGTTGCCAGTACCACTCCACTGCCTCCACCTAAACCATCTCCT
  GCTAACCCCATCACTGTGGAAATGAAAGGCCTGAAGACAGATTTGGACCTTCAGCAGTAC
  AGCTTTATAAATCAGATGTGTTATGAGCGAGCCCTCCACTGGTATGCCAAGTATTTCCCT
  TACCTTGTCCTCATCCATACCCTGGTCTTTATGCTCTGCAGTAACTTTTGGTTCANATTC
  CCTGGTTCCAGCTCCAAAATAGAACATTTCATCTCCATTCTGGGGAAAGTNNGTTTGACT
  CTCCTTGGACCACACGGGCTTTATCTGAAGTGTCTGGNGAGGACTCAGAAGAAAGGACAC
  AGGAAGAACACATGAACGGTCCAACCCATCCATCTGGTCAGAGGCAGCCTGGTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032270 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGAAAAAAACAATTAAAT
  TTTTATTAAAAATACATACNTCCTTACGCGATCTTTGTGTACATTTTGTGTGAAAAGGAA
  AAAAAAATAGGAAAGGCATTAAGACTACCTAATTATTTATACTGTATTTGGTAGAAGCGT
  GTTTCAGTCAAACTTTAACGAAAAATAAGTTATTCTGTTTTCATTTGCTCCCGGACGTCA
  GAAGGCAGAGTTTCAAACAGAGCATCTTCTACAACTAAACCAGCTCGCTTCAGAGCCCGA
  CAGTCACCCAGTTCAGGAGGGAGGATTTCAAAGTGATTACCTTTTACATCTAAGTAGGAA
  AGAAATAGCAAATTTCCAATTTTCGGTGAAAGTACAGATAGGCTGTTTTTTCCAATCTTC
  AGAGTTTTAAGTTTCTTGCAGAAGTAGAGTTCATCTGGAAGGCTTTCCACTTTGTTACAT
  GTGATGGAAAAATACTGTAAACTTTGTAGAACTCCAATTTCAGGGGGGATAAATCGAATG
  TCATTGTACGATAAGTCCAAGTATCGGATCTTGTTGCATAGGAAGAGGTGGGAAGGCAGC
  ACCTCTATTTTATTGTGACTAAAGGACAGGCGTTCCAGGCTGGTGAGTTTCTTTATATGC
  TCTGGGATGTANGTGATGCTGTTATGCCACAGTTNTAGCACTGTCAACTTTCTTAAGTGC
  TGAAAGCTAACGATTTCTTCTATAGATTTCAGATTGGTTTCCTTCAGGTCCCATTCCTGG
  AGGCTGAGTANGCTGAACACAGCATGAGGGATACGCTNCAGNTCACAGTGGNACAGCTNC
  AGCTCTGTCAGATGGNTCATCTTCTTTTAGNNTGTGAGCATNACAGACTTGGNTGCCATC
  ATTATGTATGCACATCTTCTGGGAAATGGNCTGAAACATTCCCCACTGGNCTGAGGATTN
  TGGNAAACNNTGCTTTGATNNAGAGAATTTTAGGCTTTTTGAAATCCGACAGACCTCAGG
  GGGGAACTTCTGGGATATATGATCTAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032270
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032270.2, NP_115646.2
• RefSeq Size: 2575 bp
• RefSeq ORF: 2412 bp
• Locus ID: 84230
• UniProt ID: Q8TDW0
• Cytogenetics: 1p22.2
• Domains: LRR, LRR_TYP, LRR_PS
• Protein Families: Transmembrane
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.[UniProtKB/Swiss-Prot Function]