PAMM (FAM213A) (NM_032333) Human Untagged Clone

SKU SC105291

FAM213A (untagged)-Human chromosome 10 open reading frame 58 (C10orf58), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PAMM
• Synonyms: Adrx; C10orf58; FAM213A; PAMM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105291 sequence for NM_032333 edited (data generated by NextGen Sequencing); ATGTCTTTCCTCCAGGACCCAAGTTTCTTCACCATGGGGATGTGGTCCATTGGTGCAGGA; GCCCTGGGGGCTGCTGCCTTGGCATTGCTGCTTGCCAACACAGACGTGTTTCTGTCCAAG; CCCCAGAAAGCGGCCCTGGAGTACCTGGAGGATATAGACCTGAAAACACTGGAGAAGGAA; CCAAGGACTTTCAAAGCAAAGGAGCTATGGGAAAAAAATGGAGCTGTGATTATGGCCGTG; CGGAGGCCAGGCTGTTTCCTCTGTCGAGAGGAAGCTGCGGATCTGTCCTCCCTGAAAAGC; ATGTTGGACCAGCTGGGCGTCCCCCTCTATGCAGTGGTAAAGGAGCACATCAGGACTGAA; GTGAAGGATTTCCAGCCTTATTTCAAAGGAGAAATCTTCCTGGATGAAAAGAAAAAGTTC; TATGGTCCACAAAGGCGGAAGATGATGTTTATGGGATTTATCCGTCTGGGAGTGTGGTAC; AACTTCTTCCGAGCCTGGAACGGAGGCTTCTCTGGAAACCTGGAAGGAGAAGGCTTCATC; CTTGGGGGAGTTTTCGTGGTGGGATCAGGAAAGCAGGGCATTCTTCTTGAGCACCGAGAA; AAAGAATTTGGAGACAAAGTAAACCTACTTTCTGTTCTGGAAGCTGCTAAGATGATCAAA; CCACAGACTTTGGCCTCAGAGAAAAAATGA; ; Clone variation with respect to NM_032333.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032333 unedited; NGGTCAACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCGGGGC; GGTAAAAGGCCGGCAGAAGGGAGGCACTTGAGAAAGTCTTTCCTCCAGGACCCAAGTTTC; TTCACCATGGGGATGTGGTCCATTGGTGCAGGAGCCCTGGGGGCTGCTGCCTTGGCATTG; CTGCTTGCCAACACAGACGTGTTTCTGTCCAAGCCCCAGAAAGCGGCCCTGGAGTACCTG; GAGGATATAGACCTGAAAACACTGGAGAAGGAACCAAGGACTTTCAAAGCAAAGGAGCTA; TGGGAAAAAAATGGAGCTGTGATTATGGCCGTGCGGAGGCCAGGCTGTTTCCTCTGTCGA; GAGGAAGCTGCGGATCTGTCCTCCCTGAAAAGCATGTTGGACCAGCTGGGCGTCCCCCTC; TATGCAGTGGTAAAGGAGCACATCAGGACTGAAGTGAAGGATTTCCAGCCTTATTTCAAA; GGAGAAATCTTCCTGGATGAAAAGAAAAAGTTCTATGGTCCACAAAGGCGGAAGATGATG; TTTATGGGATTTATCCGTCTGGGAGTGTGGTACAACTTCTTCCGAGCCTGGAACGGAGGC; TTCTCTGGAAACCTGGAAGGAGAAGGCTTCATCCTTGGGGGAGTTTTCGTGGTGGGATCA; GGAAAGCAGGGCATTCTTCTTGAGCACCGAGAAAAAGAATTTGGAGACAAAGTAAACCTA; CTTTCTGTTCTGGAAGCTGCTAAGATGATCAAACCACAGACTNTGGCCTCAGAGAAAAAT; GATTGTGTGAAACTGCCCAGCTCAGGGATAACCAGNGCATTCACCTGTGTCATGGGAATG; NNATGNTTCCACTCGTGTCCTTNAGGAGTGAGAACCCATTATACTCTACTTANNATGATT; AT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032333 unedited; CTTGGCCGCGGCCGCATCTAAATCGGTTTTTTTTTTTTTTTTTTATTAGATCTCATCACC; CAGCTGGAATACAGTGGTGCGATCTCAGCTCACCGCAACCTCCACCTCCCAGGTTCAAGT; GATTCTCCTGCCTCAGCCTCCCGGGTAGCTGGGACCACAGGTGCCTGCCACCACACCCGG; GTGATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGCTCAGGCTGGTCTCGAA; CTCCCGACCTCAAGTGACTTGCTCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTG; AGCCACTGCACCCAGCCTGGCAGTCAATTTTAAGCCTCCTATTTCCCAGGTTTTAGCTTA; ATAATCCTCATTAGTTTTTCAGATTTTTGCCAGTTTTGTTTTGGGGCTATTTTGCCTTAG; TGGGCCTAAACAGAATATTAAAATACATTAATAATCCATACTGACAGTAGAGTCTAAATG; GGTTTCTCACTCCTTAGGGACCCGAGTGGAAACCATACTTCCCATGACACCAGGGAATGC; CCCTGGCTACCCCTGACCGGGCAGTTGGCCCAATCATTTTTTCTCTGAGGCCCAACCCGC; GGCTTGCCCTCTTCACCCCTTCCCCAACAAAACCAGGTGCCTTTCGCGCAAACCGTTCCG; CGCGCGCTCAAAACATGCCGCGCTTCCCTCCCCCCGGTGCGGAACTTCCCCCCGCTCCCC; CCTTCTCTCCTATCTTCCCCCCCCCCCCCCCCCCCCTCCGTGTATCTCCCCTGCCCCCCC; CTCACCTCCCCGCATTACCTCCACCTTCTCGCGCAGCGCCCACCTTCTCCCTTATCTTCC; CCTTCGCTTGGCTCGTTCTTTCCCTACCCGGCCCCGCCGCTCCCATTCTCTCGCCTGCCC; CTCCCCTGCCTCCATCCCTCCCCCCCCCCTCCCCCCCCCTCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_032333
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032333.2, NP_115709.2
• RefSeq Size: 1690 bp
• RefSeq ORF: 690 bp
• Locus ID: 84293
• UniProt ID: Q9BRX8
• Cytogenetics: 10q23.1
• Protein Families: Secreted Protein, Transmembrane
• Summary: Involved in redox regulation of the cell (PubMed:26438880, PubMed:19951071). Acts as an antioxidant (PubMed:19951071, PubMed:26438880). Inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation (PubMed:19951071). May affect bone resorption and help to maintain bone mass (PubMed:19951071). Acts as a negative regulator of macrophage-mediated inflammation by inhibiting macrophage production of inflammatory cytokines, probably through suppression of the MAPK signaling pathway (PubMed:26438880).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2, 3 and 4 encode the same isoform.