PAMM (FAM213A) (NM_032333) Human Untagged Clone

CAT#: SC105291

FAM213A (untagged)-Human chromosome 10 open reading frame 58 (C10orf58), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAMM
• Synonyms: Adrx; C10orf58; FAM213A; PAMM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105291 sequence for NM_032333 edited (data generated by NextGen Sequencing)
  ATGTCTTTCCTCCAGGACCCAAGTTTCTTCACCATGGGGATGTGGTCCATTGGTGCAGGA
  GCCCTGGGGGCTGCTGCCTTGGCATTGCTGCTTGCCAACACAGACGTGTTTCTGTCCAAG
  CCCCAGAAAGCGGCCCTGGAGTACCTGGAGGATATAGACCTGAAAACACTGGAGAAGGAA
  CCAAGGACTTTCAAAGCAAAGGAGCTATGGGAAAAAAATGGAGCTGTGATTATGGCCGTG
  CGGAGGCCAGGCTGTTTCCTCTGTCGAGAGGAAGCTGCGGATCTGTCCTCCCTGAAAAGC
  ATGTTGGACCAGCTGGGCGTCCCCCTCTATGCAGTGGTAAAGGAGCACATCAGGACTGAA
  GTGAAGGATTTCCAGCCTTATTTCAAAGGAGAAATCTTCCTGGATGAAAAGAAAAAGTTC
  TATGGTCCACAAAGGCGGAAGATGATGTTTATGGGATTTATCCGTCTGGGAGTGTGGTAC
  AACTTCTTCCGAGCCTGGAACGGAGGCTTCTCTGGAAACCTGGAAGGAGAAGGCTTCATC
  CTTGGGGGAGTTTTCGTGGTGGGATCAGGAAAGCAGGGCATTCTTCTTGAGCACCGAGAA
  AAAGAATTTGGAGACAAAGTAAACCTACTTTCTGTTCTGGAAGCTGCTAAGATGATCAAA
  CCACAGACTTTGGCCTCAGAGAAAAAATGA
  
  Clone variation with respect to NM_032333.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032333 unedited
  NGGTCAACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCGGGGC
  GGTAAAAGGCCGGCAGAAGGGAGGCACTTGAGAAAGTCTTTCCTCCAGGACCCAAGTTTC
  TTCACCATGGGGATGTGGTCCATTGGTGCAGGAGCCCTGGGGGCTGCTGCCTTGGCATTG
  CTGCTTGCCAACACAGACGTGTTTCTGTCCAAGCCCCAGAAAGCGGCCCTGGAGTACCTG
  GAGGATATAGACCTGAAAACACTGGAGAAGGAACCAAGGACTTTCAAAGCAAAGGAGCTA
  TGGGAAAAAAATGGAGCTGTGATTATGGCCGTGCGGAGGCCAGGCTGTTTCCTCTGTCGA
  GAGGAAGCTGCGGATCTGTCCTCCCTGAAAAGCATGTTGGACCAGCTGGGCGTCCCCCTC
  TATGCAGTGGTAAAGGAGCACATCAGGACTGAAGTGAAGGATTTCCAGCCTTATTTCAAA
  GGAGAAATCTTCCTGGATGAAAAGAAAAAGTTCTATGGTCCACAAAGGCGGAAGATGATG
  TTTATGGGATTTATCCGTCTGGGAGTGTGGTACAACTTCTTCCGAGCCTGGAACGGAGGC
  TTCTCTGGAAACCTGGAAGGAGAAGGCTTCATCCTTGGGGGAGTTTTCGTGGTGGGATCA
  GGAAAGCAGGGCATTCTTCTTGAGCACCGAGAAAAAGAATTTGGAGACAAAGTAAACCTA
  CTTTCTGTTCTGGAAGCTGCTAAGATGATCAAACCACAGACTNTGGCCTCAGAGAAAAAT
  GATTGTGTGAAACTGCCCAGCTCAGGGATAACCAGNGCATTCACCTGTGTCATGGGAATG
  NNATGNTTCCACTCGTGTCCTTNAGGAGTGAGAACCCATTATACTCTACTTANNATGATT
  AT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032333 unedited
  CTTGGCCGCGGCCGCATCTAAATCGGTTTTTTTTTTTTTTTTTTATTAGATCTCATCACC
  CAGCTGGAATACAGTGGTGCGATCTCAGCTCACCGCAACCTCCACCTCCCAGGTTCAAGT
  GATTCTCCTGCCTCAGCCTCCCGGGTAGCTGGGACCACAGGTGCCTGCCACCACACCCGG
  GTGATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGCTCAGGCTGGTCTCGAA
  CTCCCGACCTCAAGTGACTTGCTCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTG
  AGCCACTGCACCCAGCCTGGCAGTCAATTTTAAGCCTCCTATTTCCCAGGTTTTAGCTTA
  ATAATCCTCATTAGTTTTTCAGATTTTTGCCAGTTTTGTTTTGGGGCTATTTTGCCTTAG
  TGGGCCTAAACAGAATATTAAAATACATTAATAATCCATACTGACAGTAGAGTCTAAATG
  GGTTTCTCACTCCTTAGGGACCCGAGTGGAAACCATACTTCCCATGACACCAGGGAATGC
  CCCTGGCTACCCCTGACCGGGCAGTTGGCCCAATCATTTTTTCTCTGAGGCCCAACCCGC
  GGCTTGCCCTCTTCACCCCTTCCCCAACAAAACCAGGTGCCTTTCGCGCAAACCGTTCCG
  CGCGCGCTCAAAACATGCCGCGCTTCCCTCCCCCCGGTGCGGAACTTCCCCCCGCTCCCC
  CCTTCTCTCCTATCTTCCCCCCCCCCCCCCCCCCCCTCCGTGTATCTCCCCTGCCCCCCC
  CTCACCTCCCCGCATTACCTCCACCTTCTCGCGCAGCGCCCACCTTCTCCCTTATCTTCC
  CCTTCGCTTGGCTCGTTCTTTCCCTACCCGGCCCCGCCGCTCCCATTCTCTCGCCTGCCC
  CTCCCCTGCCTCCATCCCTCCCCCCCCCCTCCCCCCCCCTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032333
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032333.2, NP_115709.2
• RefSeq Size: 1690 bp
• RefSeq ORF: 690 bp
• Locus ID: 84293
• UniProt ID: Q9BRX8
• Cytogenetics: 10q23.1
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: Involved in redox regulation of the cell (PubMed:26438880, PubMed:19951071). Acts as an antioxidant (PubMed:19951071, PubMed:26438880). Inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation (PubMed:19951071). May affect bone resorption and help to maintain bone mass (PubMed:19951071). Acts as a negative regulator of macrophage-mediated inflammation by inhibiting macrophage production of inflammatory cytokines, probably through suppression of the MAPK signaling pathway (PubMed:26438880).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2, 3 and 4 encode the same isoform.