FRG1 (AK057099) Human Untagged Clone

CAT#: SC102079

(untagged)-Human cDNA FLJ32537 fis, clone SMINT2000400, highly similar to Human FRG1 mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FRG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102079 sequence for AK057099 edited (data generated by NextGen Sequencing)
  ATGGATGAGGGAACCTATATACATGCACTCGATAATGGTCTTTTTACCCTGGGAGCTCCA
  CACAAAGAAGTTGATGAGGGCCCTAGTCCTCCAGAGCAGTTTTCGGCTGTCAAATTATCT
  GATTCCAGAATCACCCTGAAATCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAA
  CTTGTTGTTGGGCGTTCAGATGCAATTGGACCAAGAGAACAATGGGAACCAGTCTTTAAA
  ATTGGGAAAATGGCTTTGTCGACCTCAAATAGCTGCTTTATTAGATACAGTGAAGCAGAG
  GACATAGAAGCAAAAAGTAAAACAGCAGGAGAAGAAGAAATGATCAAGAGTCTCAGTCAT
  AGCCTGCCTTCATCTCTGAAGGGACCAGGCGACCCACGGAGTGCCTCGGCAGCCTTGTTA
  TTGCATGCAATCTTCACAGACAAGATCTCACTGCAACCAGGTTTCTACAGGAGCACAGGT
  GTGACATTATAG
  
  Clone variation with respect to AK057099.1
  371 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK057099 unedited
  GGGTTCACAAATGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTCCGCGC
  AGAAGTCTCCCGGAGCCGACTTCTGAGAGTCGAGTACTCCTATGTTAAGACTACCAAGCT
  CGTGTTCAAGGGAACCAAGGCGAAGAGGCGAGATGGAAAGAAAGTATCAAGGAGTGAGCT
  GAAGCCACTGCCCTTGAGAACCCTCTCGAGGAGTCTGGGCTCATGAGGATGCCAGAATAA
  GTGGCAGTAAGAAGAAAAAGAACAAAGATAAGAAAAGAAAAAGAGAAGAAGATGAAGAAA
  CCCAGCTTGATATGTTGGAATCTGGTGGACAGTAACAAACTTTGGTGAAAATTTCAGGAA
  CCATAGCCATTGAAATGGATGAGGGAACCTATATACATGCACTCGATAATGGTCTTTTTA
  CCCTGGGAGCTCCACACAAAGAAGTTGATGAGGGCCCTAGTCCTCCAGAGCAGTTTTCGG
  CTGTCAAATTATCTGATTCCAGAATCACCCTGAAATCTGGCTATGGAAAATATCTTGGTA
  TAAATTCAGATGAACTTGTTGTTGGGCGTTCAGATGCAATTGGACCAAGAGAACAATGGG
  AACCAGTCTTTAAAATTGGGAAAATGGCTTTGTCGACCTCAAATAGCTGCTTTATTAGAT
  ACAGTGAAGCAGAGGACATAGAAGCCAAAAGTAAAACAGCAGGAGAAGAAGAAATGATCA
  AGAGTCTCAGTCATAGCCTGCCTTCATCTCTGAAGGGACCANGCGACCCACGGAGTGCCT
  TCGCAGCCTTGTTATTGCATGCAATCTTCACAGACAAGATCTCACTGCAACCCAGTTTCT
  ACAGGAGCACAGGTGTGACATTATAGTGATGACATCATTCTCAGAGAAAATCATTTTACG
  CACTAAGGACCACAGNTTCAAGGNAGCTTACACAAAGGGATGAC
  
• Restriction Sites: NotI-NotI
• ACCN: AK057099
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK057099.1, BAB71366.1
• RefSeq Size: 2306 bp
• RefSeq ORF: 492 bp
• Locus ID: 2483
• Cytogenetics: 4q35.2
• Gene Summary: This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008]