CARD10 (NM_014550) Human Untagged Clone

CAT#: SC101447

CARD10 (untagged)-Human caspase recruitment domain family, member 10 (CARD10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARD10
• Synonyms: BIMP1; CARMA3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014550 edited
  GAATTCGGCACGAGGGGGAGCCCGAGGCGACTGTAGCGTGCGGGACCCTGAGGACACGGC
  CATGCCGGGCCGGGCGGAGGCGGGGGAGGCCGAGGAGGAGGCCGGGGCCGGCTCGGGGTC
  TGAGGCGGAGGAGGACGCGCTGTGGGAGCGAATCGAGGGCGTCCGGCATCGGCTGGCTCG
  CGCCCTGAACCCGGCCAAGCTCACGCCGTATCTGCGCCAGTGCCGGGTCATCGACGAGCA
  GGACGAGGAGGAGGTGCTGAGCACCTACCGCTTCCCGTGCCGCGTCAACCGCACCGGGCG
  CCTGATGGACATCTTGCGCTGCCGTGGCAAGAGGGGCTATGAGGCCTTCCTGGAAGCCCT
  GGAGTTCTACTACCCCGAACACTTCACGCTGCTCACGGGCCAGGAACCCGCCCAGCGCTG
  CTCCATGATCCTCGATGAGGAGGGGCCTGAGGGCCTGACCCAATTCTTGATGACAGAGGT
  GCGACGGCTGCGGGAAGCTCGCAAGAGCCAGCTGCAGCGGGAGCAGCAACTGCAGGCCCG
  GGGCCGGGTGCTCGAGGAGGAGCGGGCAGGGCTGGAGCAGCGGCTGCGGGACCAGCAGCA
  GGCTCAGGAGCGCTGTCAACGGCTGCGGGAGGACTGGGAGGCGGGCAGCCTGGAGCTGCT
  GCGGCTCAAGGATGAGAACTACATGATCGCCATGCGCCTGGCACAGCTCAGTGAGGAGAA
  GAACTCGGCTGTACTTCGCAGCCGTGACCTGCAGCTGGCGGTGGATCAGCTCAAGCTCAA
  AGTGAGTCGGCTGGAGGAAGAGTGTGCACTGCTTCGAAGGGCCAGGGGCCCGCCCCCTGG
  GGCAGAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGCCAGACAATGTGGACCTTGT
  CTCTGAGCTGCGTGCTGAGAACCAGCGGCTGACGGCGTCACTGCGGGAGTTGCAGGAGGG
  CCTGCAGCAGGAGGCGAGCCGGCCGGGGGCCCCGGGCTCCGAGCGCATCCTGCTGGACAT
  CCTAGAGCATGACTGGCGGGAGGCGCAGGACAGCAGGCAGGAGCTGTGCCAGAAGCTGCA
  TGCCGTGCAGGGGGAGCTGCAGTGGGCCGAGGAGCTGCGCGACCAGTACCTGCAGGAGAT
  GGAAGACCTGCGGCTCAAGCACCGCACGCTGCAGAAGGACTGTGACCTGTACAAGCACCG
  CATGGCCACTGTCCTGGCCCAACTGGAGGAGATTGAGAAGGAGCGAGACCAGGCCATCCA
  GAGCCGTGACCGGATCCAGTTGCAGTACTCACAGAGCCTCATCGAGAAGGACCAGTACCG
  CAAGCAGGTGCGGGGCCTGGAGGCGGAGCGGGATGAGCTGCTGACAACGCTCACCAGCCT
  GGAGGGCACCAAGGCTCTGCTGGAGGTTCAGCTGCAGCGGGCCCAGGGTGGCACCTGCCT
  CAAGGCCTGTGCCTCCTCCCATTCCCTGTGCTCCAACCTCAGCAGCACTTGGAGCCTGAG
  CGAGTTCCCCTCCCCTCTGGGAGGCCCAGAAGCAACTGGGGAGGCAGCTGTCATGGGGGG
  ACCTGAGCCTCACAACTCGGAGGAAGCCACAGACAGTGAAAAGGAGATCAATCGGCTCTC
  CATCCTGCCCTTCCCCCCCAGTGCCGGCTCCATCCTCCGCCGGCAGCGTGAGGAAGACCC
  CGCACCCCCTAAGAGATCCTTCAGCAGCATGTCAGACATCACAGGGAGTGTGACACTTAA
  GCCCTGGTCCCCTGGCCTCTCTTCGTCCTCATCCTCTGACAGCGTGTGGCCTTTGGGAAA
  GCCGGAAGGCCTCCTGGCTCGGGGCTGTGGCCTGGACTTCCTCAACAGGTCTCTGGCTAT
  TCGGGTGTCTGGCCGGAGCCCCCCAGGGGGCCCAGAGCCGCAGGACAAGGGACCAGATGG
  ACTGTCGTTTTATGGGGACAGATGGTCTGGGGCTGTGGTGCGCAGGGTGCTGTCTGGGCC
  TGGGTCCGCCAGGATGGAACCAAGAGAGCAAAGGGTGGAAGCTGCTGGTCTGGAGGGGGC
  GTGCCTGGAAGCCGAGGCCCAGCAGAGAACCTTGCTCTGGAATCAGGGGTCCACACTCCC
  CTCCCTGATGGACTCGAAGGCCTGCCAGTCCTTCCACGAGGCCCTAGAAGCCTGGGCAAA
  GGGACCAGGTGCCGAGCCCTTCTACATTCGTGCCAACCTCACCTTGCCTGAGAGGGCAGA
  TCCCCATGCCCTTTGCGTGAAAGCCCAAGAGATCCTTCGACTGGTGGACTCGGCATACAA
  GCGGAGGCAGGAATGGTTCTGCACCCGGGTTGACCCCCTCACTCTGCGGGACCTGGACCG
  GGGCACCGTGCCCAATTATCAGAGAGCCCAGCAGCTCCTAGAAGTTCAGGAGAAATGCCT
  GCCCTCCAGCCGGCACCGAGGCCCCCGCAGTAATCTGAAGAAGAGAGCCCTGGACCAGCT
  GCGGCTGGTGAGGCCCAAGCCCGTGGGGGCGCCTGCAGGGGACTCCCCGGATCAGCTGCT
  GCTGGAGCCCTGTGCAGAGCCGGAGCGGAGCCTCAGACCCTACAGTTTGGTGCGGCCGCT
  ACTGGTGTCTGCCCTGCGGCCCGTGGTGCTGTTGCCTGAGTGCCTGGCGCCCCGGCTCAT
  CCGTAACCTGCTAGACCTGCCCAGCTCCCGGCTGGACTTCCAAGTGTGCCCAGCGGAAAG
  CCTCTCTGGGGAGGAACTGTGCCCATCCTCAGCGCCTGGAGCCCCCAAGGCTCAGCCTGC
  CACCCCTGGGCTAGGCAGCAGGATCCGGGCCATCCAGGAGTCTGTTGGGAAGAAGCACTG
  CCTGCTGGAGCTGGGTGCTCGGGGTGTGCGGGAGCTGGTGCAGAACGAGATCTACCCCAT
  CGTCATCCACGTGGAGGTGACTGAGAAGAATGTCCGGGAAGTCAGGGGTCTGCTGGGCCG
  GCCGGGCTGGCGGGACTCAGAGCTGCTGCGGCAGTGCCGTGGCTCAGAGCAGGTGCTCTG
  GGGGCTGCCCTGCTCCTGGGTGCAGGTGCCCGCCCATGAGTGGGGACACGCAGAGGAGCT
  GGCCAAGGTGGTGCGCGGCCGCATCCTGCAGGAGCAGGCCCGCCTCGTGTGGGTGGAGTG
  CGGCAGCAGCAGAGGCTGCCCCAGCAGCAGTGAGGCCTGAGGCTCATCTGATACCTGCAC
  CTTCTCCCCAAGCCAGCGTGGACCCTGGTGTCTATGGTGAAGCTGGGCCCTCCCACCCTG
  AGCCTTCCTAGACCCTTGGACTCTCAGATGCAGGGCCCTTGGCTCTGGCCTCTCACCCCC
  AAGGCTGTCTCTGGCCCTGCCGAGCCTATXXXXXXXXXXGGGTCTTATGTGTGTCTGTGT
  CTTCTCCTTAAACACTCGCCCTGGAGTCTGTTCTCACACCTGTGCGCAGGTTTGCACACT
  CAAGTTCTCATGGGCAGGCTCAGGTCTGTCCCGCTGCCCTGGGCACGAGGTCTCCTGAGG
  ACCTGGGCCTGTTCTGCTCCTAGGAGACCTGAGCCCGTTACCGCGTGACTCCCACCATCC
  AGCTCGCGCTCCTCGTGGATTCAGCCATGCATGGACTGGGGTGTTCCCTGGCCCATGGTC
  ACCTGTGCCCCTCGTGTCTCCTCACATGGGTGTCTGTGGTTCTCTCCTGTGTAAATGTCA
  CGCCCCACCCCTGTTTCATGTGGGCACTAACACGTGTGCGTTCCTGGCGGGCACACTCAG
  GACCGTGCCTCACAGGGCCCACTCCCTGCCTATGCCTCCCTCTTGGGGGGCCGAGGAGGG
  CGGCTGCTCTGTCATGAGAATGTACGGCCCGTGGATGATTAACGGGCCTTTTTCACTTAG
  AAGCTGCACATTATGGAGCATTAAACACTTTTGTCATAAAAAAAAAAAAAAAAAACTCGA
  C
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014550 unedited
  ACCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTACTATAAGCA
  GAGGCTCACGTTAGGTGACACTACGAGAGTACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGGGAAGCCCGAGGCGACTGTAGCGTGCGGGACCCTGAGGACACG
  GCCATGCCGGGCCGGGCGGAGGCGGGGGAGGCCGAGGAGGAGGCCGGGGCCGGCTCGGGG
  TCTGAGGCGGAGGAGGACGCGCTGTGGGAGCGAATCGAGGGCGTCCGGCATCGGCTGGCT
  CGCGCCCTGAACCCGGCCAAGCTCACGCCGTATCTGCGCCAGTGCCGGGTCATCGACGAG
  CAGGACGAGGAGGAGGTGCTGAGCACCTACCGCTTCCCGTGCCGCGTCAACCGCACCGGG
  CGCCTGATGGACATCTTGCGCTGCCGTGGCAAGAGGGGCTATGAGGCCTTCCTGGAAGCC
  CTGGAGTTCTACTACCCCGAACACTTCACGCTGCTCACGGGCCAGGAACCCGCCCAGCGC
  TGCTCCATGATCCTCGATGAGGAGGGGCCTGAGGGCCTGACCCAATTCTTGATGACAGAG
  GTGCGACGGCTGCGGGAAGCTCGCAAGAGCCAGCTGCAGCGGGAGCAGCAACTGCAGGCC
  CGGGGCCGGGTGCTCGAGGAGGAGCGGGCAGGGCTGGAGCAACGGCTGCGGAACAGCAGC
  AGGCTCAGGAGCGCTGNCACGGCTGCGGGAGGACTGGGAGGCNGGCAACCTGGAGCTGCT
  GGCGNTCAAGATGAGAACTCATGATCCCCATGCGCCTGGACAGGTCAGTGAGAAAGAACT
  CGGTTTACTTNNANNCCTNACCTCAGCTGGCGGGGGATAAGTTAGCTCAAGTAAATTCGT
  GGAGGAAAAGTTCACATGTTTNAAAGNCAAGGGCTCCTCCCTTGGTTAAGAANAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014550 unedited
  GGTACTAGCTATGNNACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAT
  GACAAAAGTGTTTAATGCTCCATAATGTGCAGCTTCTAAGTGAAAAAGCCCGTTAATCAT
  CCACGGGCCGTACATTCTCATGACAGAGCAGCCGCCCTCCTCGGCCCCCCAAGAGGGAGG
  CATAGGCAGGGAGTGGGCCCTGTGAGGCACGGTCCTGAGTGTGCCCGCCAGGAACGCACA
  CGTGTTAGTGCCCACATGAAACAGGGGTGGGGCGTGACATTTACACAGGAGAGAACCACA
  GACACCCATGTGAGGAGACACGAGGGGCACAGGTGACCATGGGCCAGGGAACACCCCAGT
  CCATGCATGGCTGAATCCACGAGGAGCGCGAGCTGGATGGTGGGAGTCACGCGGTAACGG
  GCTCAGGTCTCCTAGGAGCAAAACAGGCCCAGGTCCTCACGAGACCTCGTGCCCCAGGCA
  GCGGGACAGACCTGAGCCTGCCCATGAGAACTTGAGTGTGCAAACCTGCGCACAGGTGTG
  AGAACAGACTCCAGGGCGAGTGTTTAAGGAGGAGACACAGACACCCATTAGACCCACTAG
  GGGGAATGTGGAGACCCCAGGAAAGGGGGGGGGGCCCACAACAACCAGTTAAGGGGAGTG
  GGGACTCTGTTCCCGGGGCTCCATTAGGCTCGGCAGGGCCAAAAACCACCTTGGGGGGGA
  GAGGGCCAAAACCCAAGGCCCCGTCTCTTAGAATTCCAGGGTTTAGAAAGGCCTCGGGGG
  GGAGGGCCCACCTTCCCATAAACCCAGGGTCCACCCGGCTTGGGAAAAGAGGCNGTATTA
  AAAAAACTCCAGGCCCCTTGGGTGTGGGGGGACCCCTGTGGTGCCGCCCTCCCCCCCCAG
  AGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014550
• Insert Size: 3650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014550.3, NP_055365.2
• RefSeq Size: 3912 bp
• RefSeq ORF: 3099 bp
• Locus ID: 29775
• UniProt ID: Q9BWT7
• Cytogenetics: 22q13.1
• Gene Summary: The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]