CAVIN1 (NM_012232) Human Untagged Clone

SKU SC101318

PTRF (untagged)-Human polymerase I and transcript release factor (PTRF)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CAVIN1
• Synonyms: CAVIN; cavin-1; CGL4; FKSG13; PTRF
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101318 sequence for NM_012232 edited (data generated by NextGen Sequencing); ATGGAGGACCCCACGCTCTATATTGTCGAGCGGCCGCTTCCCGGGTNNNCCGACGCCGAG; GCCCCGGAGCCTTCCTCCGCTGGGGCTCAGGCAGCGGAGGAGCCGTCGGGGGCCGGCTCA; GAAGAGCTGATCAAGTCGGACCAGGTGAACGGCGTGCTGGTGCTGAGCCTCCTGGACAAA; ATCATCGGGGCCGTAGACCAGATCCAGCTGACTCAAGCACAGCTGGAGGAGCGGCAGGCG; GAGATGGAGGGCGCAGTGCAGAGCATCCAGGGCGAGCTGAGCAAGCTGGGCAAGGCGCAC; GCCACCACGAGCAATACGGTGAGCAAGCTGCTGGAGAAGGTGCGCAAGGTCAGCGTCAAC; GTGAAGACCGTGCGCGGCAGCCTGGAGCGCCAGGCGGGGCAGATCAAGAAGCTGGAGGTC; AACGAGGCCGAGCTGCTGCGGCGCCGCAACTTTAAAGTCATGATCTACCAGGATGAAGTG; AAGCTGCCGGCCAAACTGAGCATCAGCAAATCGCTGAAAGAGTCGGAGGCGCTGCCAGAG; AAGGAGGGCGAGGAGCTGGGCGAGGGCGAGCGGCCCGAGGAGGACGCAGCGGCGCTGGAG; CTTTCGTCGGACGAGGCGGTGGAGGTTGAGGAGGTTATTGAGGAGTCCCGCGCAGAGCGT; ATCAAGCGCAGCGGCCTGCGGCGCGTGGACGACTTCAAGAAGGCCTTCTCCAAGGAGAAG; ATGGAGAAGACCAAGGTGCGTACCCGCGAGAACCTGGAGAAGACGCGCCTCAAGACCAAG; GAAAACCTGGAGAAGACGCGGCACACCCTGGAGAAGCGCATGAACAAGCTGGGCACGCGC; CTGGTGCCCGCCGAGCGGCGCGAGAAACTGAAGACGTCGCGGGACAAGTTGCGCAAATCC; TTCACGCCCGACCACGTGGTGTACGCGCGCTCCAAGACCGCGGTCTACAAGGTGCCACCC; TTCACCTTCCACGTCAAGAAGATCCGCGAGGGCCAGGTGGAAGTGCTCAAGGCCACCGAG; ATGGTGGAGGTGGGCGCCGACGACGACGAGGGCGGCGCGGAGCGCGGGGAGGCCGGCGAC; CTGCGGCGCGGGAGCAGCCCCGACGTGCACGCGCTGCTGGAGATCACCGAGGAGTCGGAC; GCCGTGCTGGTGGACAAGAGCGACAGCGACTGA; ; Clone variation with respect to NM_012232.5; 47 a=>n;48 c=>n;49 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012232 unedited; CAATCCCCCGCCCGTTGACGCAATGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAAG; CAGAGCTCATTTAGGTGACACTATAGGGGACAGGCTACTTGTTCTTTTTGCAGCGGCCGC; GAATTCGGCACGAGGGTCCCGGTGCGCACGGACGTGGCTCGAGTTTCCTCTGCTCTCCGC; TCTCGCCCGCTAGCTCTCCTCCCTTCCGCTCCTGCTTCTCTCCGGGTCTCCCGCTCCAGC; TCCAGGGCGACCCGGCCGGTCCCGCACGGCTCCGGGTAGCCATGGAGGACCCCACGCTCT; ATATTGTCGAGCGGCCGCTTCCCGGGTACCCCGACGCCGAGGCCCCGGAGCCTTCCTCCG; CTGGGGCTCAGGCAGCGGAGGAGCCGTCGGGGGCCGGCTCAGAAGAGCTGATCAAGTCGG; ACCAGGTGAACGGCGTGCTGGTGCTGAGCCTCCTGGACAAAATCATCGGGGCCGTAGACC; AGAGCCAGCTGACTCAAGCACAGCTGGAGGAGCGGCAGGCGGAGATGGAGGGCGCAGTGC; AGAGCATCCAGGGCGAGCTGAGCAAGCTGGGCAAGGCGCACGCCACCACGAGCAATACGG; TGAGCAAGCTGCTGGAGAAGGTGCGCAAGGTCAGCGTCAACGTGAAGACCGTGCGCGGCA; GCCTGGAGCGCCAGGCGGNGCAGATCAAGAAGCTGGAGGTCAACGAGGCCGAGCTGCTGC; GGCGCCGCAACTTTAAAGTCATGATCTACCAGGATGAAGTGAAGCTGCCTGCCAAACTGA; GCATCAGCAAATCGCTGAAAGAGTCTGAGGCGCTGCCANAAACNGAGGCTAGGAGCATGG; CTAGGGCTAGCTGCCCTAGGAGACGCACGGCGCTGGAGCTATCTCGACGAGCGGTGGAGG; TTAGGAGGTATTGAGGAGTCCTCGCANACGTATCAGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012232 unedited; AAACTTTTGANAGACAGTTTATTGGTCGGGCTGCTCACCAGGACACAGCAACGTGAGCAG; TTCCCCAAGCCCCATAAAATGCATCTGCCCACAGCTCAGGCCCCTTCAGGCCATCAGCAC; CAAGGGACCTTGTCCCACAATCCCCAACCTCCCTCGGCAGAGGGGTCTTCAGCCATTCAG; AGGAGAGAAGAGAACCGAGAAAGGGAAAAGGAAGAAAAAAAAAAAAAAAGCAAAGCTTTG; TATTGTATAAAAGGTTTGTGTCCCCAGGCTCCCTCCCCCAATCCCTTAAAACAATGAACT; GCAGTTCTAAAAAGCAGGGCAGAGAAGGTAAGGAGCAGGTGGGGGGAAGGAGGAAGCTCT; CGGGGCCTCCCTTCAGGCTGTGACTGGGGAGAGGGGCTGTTCTTGCTTCTGACAAACCCC; CTTTAATGGGGAGGAACAAGGGGACTCGTGTCTTGAGAACCTGGTCGTGTCTTGAGAACC; CAGTCCGAACAGAATCAGGCCTCTGGACTGGGAGCAACACTCCCTTCACCCCCAAAGATT; CAGGAAAAGCACCCCAAGGACAAGGAAACCAATGAGGTCTGGGCTAGCTCTGCAGCTTTA; GGATACTAGCTCTAGGGAAGGATTTTTTCCTTTTTAAACAGCGTCTCACTCTGTTGCCTA; GGATGGAGCACAGAGGCACCCTCATAGCTCACTGCAGCCTCAAACTCCTGGGCTCTGGCG; ATCCTCCCGCCTCAGCCTCCCAAGTAGCTGAGACCACAGGCACGTGCCACCATGCTCCTA; GGGAAGGAGCTTGAGAATGAACTGCCAGGAGTGAACCAGGGCTGGCTGCTCTGTGATGTT; CTCTCCCCACCTCCCCTTCAGCTCTCAACTTGGTGGCAGGGCCGGCACCCTGCTCTNCCT; CCTAACTNCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_012232
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012232.2, NP_036364.2
• RefSeq Size: 3580 bp
• RefSeq ORF: 1173 bp
• Locus ID: 284119
• UniProt ID: Q6NZI2
• Cytogenetics: 17q21.2
• Protein Families: Transcription Factors
• Summary: This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]