Phospholipase A2 (PLA2G4A) (NM_024420) Human Untagged Clone

CAT#: SC100383

PLA2G4A (untagged)-Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phospholipase A2
• Synonyms: cPLA2; cPLA2-alpha; GURDP; PLA2G4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100383 sequence for NM_024420 edited (data generated by NextGen Sequencing)
  ATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATTCCCACAAGTTT
  ACGGTAGTGGTGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTGACATGCTTGAT
  ACTCCAGATCCCTATGTGGAACTTTTTATCTCTACAACCCCTGACAGCAGGAAGAGAACA
  AGACATTTCAATAATGACATAAACCCTGTGTGGAATGAGACCTTTGAATTTATTTTGGAT
  CCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATGCCAATTATGTCATGGATGAA
  ACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAAAGAAAGAAGTT
  CCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTGAAGTTTGCTCA
  TGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTTTCAGACAACAG
  AGAAAAGAACACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGAATAGTGAAGGA
  TTGCATTCTGCACGTGATGTGCCTGTGGTAGCCATATTGGGTTCAGGTGGGGGTTTCCGA
  GCCATGGTGGGATTCTCTGGTGTGATGAAGGCATTATACGAATCAGGAATTCTGGATTGT
  GCTACCTACGTTGCTGGTCTTTCTGGCTCCACCTGGTATATGTCAACCTTGTATTCTCAC
  CCTGATTTTCCAGAGAAAGGGCCAGAGGAGATTAATGAAGAACTAATGAAAAATGTTAGC
  CACAATCCCCTTTTACTTCTCACACCACAGAAAGTTAAAAGATATGTTGAGTCTTTATGG
  AAGAAGAAAAGCTCTGGACAACCTGTCACCTTTACTGATATCTTTGGGATGTTAATAGGA
  GAAACACTAATTCATAATAGAATGAATACTACTCTGAGCAGTTTGAAGGAAAAAGTTAAT
  ACTGCACAATGCCCTTTACCTCTTTTCACCTGTCTTCATGTCAAACCTGACGTTTCAGAG
  CTGATGTTTGCAGATTGGGTTGAATTTAGTCCATACGAAATTGGCATGGCTAAATATGGT
  ACTTTTATGGCTCCCGACTTATTTGGAAGCAAATTTTTTATGGGAACAGTCGTTAAGAAG
  TATGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGCAGTGCCTTTTCCATATTG
  TTCAACAGAGTTTTGGGCGTTTCTGGTTCACAAAGCAGAGGCTCCACAATGGAGGAAGAA
  TTAGAAAATATTACCACAAAGCATATTGTGAGTAATGATAGCTCGGACAGTGATGATGAA
  TCACACGAACCCAAAGGCACTGAAAATGAAGATGCTGGAAGTGACTATCAAAGTGATAAT
  CAAGCAAGTTGGATTCATCGTATGATAATGGCCTTGGTGAGTGATTCAGCTTTATTCAAT
  ACCAGAGAAGGACGTGCTGGGAAGGTACACAACTTCATGCTGGGCTTGAATCTCAATACA
  TCTTATCCACTGTCTCCTTTGAGTGACTTTGCCACACAGGACTCCTTTGATGATGATGAA
  CTGGATGCAGCTGTAGCAGATCCTGATGAATTTGAGCGAATATATGAGCCTCTGGATGTC
  AAAAGTAAAAAGATTCATGTAGTGGACAGTGGGCTCACATTTAACCTGCCGTATCCCTTG
  ATACTGAGACCTCAGAGAGGGGTTGATCTCATAATCTCCTTTGACTTTTCTGCAAGGCCA
  AGTGACTCTAGTCCTCCGTTCAAGGAACTTCTACTTGCAGAAAAGTGGGCTAAAATGAAC
  AAGCTCCCCTTTCCAAAGATTGATCCTTATGTGTTTGATCGGGAAGGGCTGAAGGAGTGC
  TATGTCTTTAAACCCAAGAATCCTGATATGGAGAAAGATTGCCCAACCATCATCCACTTT
  GTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCTCCAGGTGTTCCAAGGGAAACTGAG
  GAAGAGAAAGAAATCGCTGACTTTGATATTTTTGATGACCCAGAATCACCATTTTCAACC
  TTCAATTTTCAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCACTTCAAT
  ACTCTGAACAACATTGATGTGATAAAAGAAGCCATGGTTGAAAGCATTGAATATAGAAGA
  CAGAATCCATCTCGTTGCTCTGTTTCCCTTAGTAATGTTGAGGCAAGAAGATTTTTCAAC
  AAGGAGTTTCTAAGTAAACCCAAAGCATAG
  
  Clone variation with respect to NM_024420.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024420 unedited
  GGGTTCAGAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAGATTCTC
  AGGTTTTAAAGACGCTAGAGTGCCAAAGAAGACTTTGAAGTGTGAAAACATTTCCTGTAA
  TTGAAACCAAAATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATT
  CCCACAAGTTTACGGTAGTGGTGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTG
  ACATGCTTGATACTCCAGATCCCTATGTGGAACTTTTTATCTCTACAACCCCTGACAGCA
  GGAAGAGAACAAGACATTTCAATAATGACATAAACCCTGTGTGGAATGAGACCTTTGAAT
  TTATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATGCCAATTATG
  TCATGGATGAAACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAA
  AGAAAGAAGTTCCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTG
  AAGTTTGCTCATGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTT
  TCAGACAACAGAGAAAAGAACACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGA
  ATAGTGAAGGATTGCATTCTGCACGTGATGTGCCTGTGGTAGCCATATTGGGTTCAGGTG
  GNGGTTTCCGAGCCATGGTGGGATTCTCTGGTGTGATGAAAGCATTATACGAATCANGAA
  TTCTGGATTGTGCTACCTACGTTGCTGGTCTTTCTGGCTCCACTGTTATATGTCAACCTT
  GTATCCTCACCCTGATTTCCAGAGAAGGGCCCNGAGAGATTATGAAGAACTATGAAAATG
  TTGCCCAATCCCCTTTACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024420 unedited
  GTCCGCGGCCGCATCTATAGTGAGTTTTTTTTTTTTTTTTTTTTATTTTTATATAGATGT
  ATTTATTTCATGTATGTATATATATGCCTATATAAATAAAAATAGTGTTGTCTCATGGTA
  TGAATAAATCTAGTCAGTGAATAACATTCATACCTAATGCGAGGTCTTATTGAGATTGAA
  CTGTTAAATATTTTAAAAGAAAAAAAATTTTTAAAAAGAACACACCTAAGAAAGTTGGTG
  AGAAATGTTTAAAAATACAGTATATATCCCTACATTTGTATCAAGAAGTTCATACTGACT
  GAAAATGTAGCTAAGTATATCCTTACATAATCAACATCATTTGTCAACCTAACTTATAAT
  AGTATTATTCTCATGCAGCTAAGTAACTGCAACTTTGAGTATCAGCCAGTCTCTCATGAT
  CAGTACGACTATCTGTACTGTACTTTTAAATCCAGTTGTCATGGGATTGCAAACTGCCTC
  AGCATCAGAAACTGCTGCCATTTCCAGTACATGAACTATGCTTTGGGTTTACTTAGAAAC
  TCCTTGTTGAAAAATCTTCTTGCCTCAACATTACTAAGGGAAACAGAGCAACGAGATGGA
  TTCTGTCTTCTATATTCAATGCTTTCAACCATGGCTTCTTTTATCACATCAATGTTGTTC
  AGAGTATTGAAGCGCATAAGATCATGTAGTCTTTTGAATGCCTGATTTGGATATTGAAAA
  TTGAAAGGTGAAAATGGCGATTCTGGGTCATCAAAAATATCANAGTCAGCGATTTCTTTC
  TCTTCCTCAGTTTCCCTAGAACACCTGNAGCCNTGTACTTTCTGAGNTGATGNTGCCANA
  ACAAGTGGATGATGGNTAGGGCATCTTCTCCTATTAGATTCTAGGTTTAAGACTANACTC
  CTTCACCCTCCGATCAAACCNTAGATCATCTTTGAAGGGGGAGCTGTCATTTACCCACTT
  NCTGCAGTAAATTCCTTGACGAGNCTANATNCCTNGNCCTGCNAAATCAAGAGATTGAAA
  TCACCCNTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024420
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024420.1, NP_077734.1
• RefSeq Size: 2875 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5321
• UniProt ID: P47712
• Cytogenetics: 1q31.1
• Domains: C2, PLA2_B
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).