Lass5 (CERS5) (NM_147190) Human Untagged Clone

CAT#: SC100282

CERS5 (untagged)-Human LAG1 homolog, ceramide synthase 5 (LASS5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lass5
• Synonyms: LASS5; Trh4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100282 sequence for NM_147190 edited (data generated by NextGen Sequencing)
  ATGGCGACAGCAGCGCAGGGACCCCTAAGCTTGCTGTGGGGCTGGCTGTGGAGCGAGCGC
  TTCTGGCTACCCGAGAACGTGAGCTGGGCTGATCTGGAGGGGCCGGCCGACGGCTACGGT
  TACCCCCGCGGCCGGCACATCCTCTCGGTGTTCCCGCTGGCGGCGGGCATCTTCTTCGTG
  AGGCTGCTCTTCGAGCGATTTATTGCCAAACCCTGTGCACTCTGTATTGGCATCGAGGAC
  AGTGGTCCTTATCAGGCCCAACCCAATGCCATCCTTGAAAAGGTGTTCATATCTATTACC
  AAGTATCCTGATAAGAAAAGGCTGGAGGGCCTGTCAAAGCAGCTGGATTGGAATGTCCGA
  AAAATCCAATGCTGGTTTCGCCATCGGAGGAATCAGGACAAGCCCCCAACGCTTACTAAA
  TTCTGTGAAAGCATGTGGAGATTCACATTTTATTTATGTATATTCTGCTATGGAATTAGA
  TTTCTCTGGTCGTCACCTTGGTTCTGGGACATCCGACAGTGCTGGCATAACTATCCATTT
  CAGCCTCTTTCAAGTGGGCTTTATCACTATTATATCATGGAATTGGCCTTCTATTGGTCC
  CTTATGTTTTCTCAGTTTACAGACATTAAAAGAAAGGACTTCCTGATCATGTTTGTGCAT
  CACTTGGTCACCATTGGGCTTATCTCCTTCTCCTACATCAACAATATGGTTCGAGTGGGA
  ACTCTGATCATGTGTCTACATGATGTCTCAGACTTCTTGCTGGAGGCAGCCAAACTGGCC
  AATTATGCCAAGTATCAGCGGCTCTGTGACACCCTTTTTGTGATCTTCAGTGCTGTTTTT
  ATGGTTACACGACTAGGAATCTATCCATTCTGGATTCTGAACACGACCCTCTTTGAGAGT
  TGGGAGATAATCGGGCCTTATGCTTCATGGTGGCTCCTCAATGGCCTGCTGCTGACCCTA
  CAGCTTCTGCATGTCATCTGGTCCTACCTAATTGCACGGATTGCTTTGAAAGCCTTGATC
  AGGGGAAAGGTATCGAAGGATGATCGCAGTGATGTGGAGAGCAGCTCAGAGGAAGAAGAT
  GTGACCACCTGCACAAAAAGTCCCTGTGACAGTAGCTCCAGCAATGGTGCCAATCGGGTG
  AATGGTCACATGGGAGGCAGCTACTGGGCTGAAGAGTAA
  
  Clone variation with respect to NM_147190.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_147190 unedited
  TTATAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTACGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGATGGCGACAGCAGCGCAGGGACCCCTAAGCTTGCTGTGGGGCT
  GGCTGTGGAGCGAGCGCTTCTGGCTACCCGAGAACGTGAGCTGGGCTGATCTGGAGGGGC
  CGGCCGACGGCTACGGTTACCCCCGCGGCCGGCACATCCTCTCGGTGTTCCCGCTGGCGG
  CGGGCATCTTCTTCGTGAGGCTGCTCTTCGAGCGATTTATTGCCAAACCCTGTGCACTCT
  GTATTGGCATCGAGGACAGTGGTCCTTATCAGGCCCAACCCAATGCCATCCTTGAAAAGG
  TGTTCATATCTATTACCAAGTATCCTGATAAGAAAAGGCTGGAGGGCCTGTCAAAGCAGC
  TGGATTGGAATGTCCGAAAAATCCAATGCTGGTTTCGCCATCGGAGGAATCAGGACAAGC
  CCCCAACGCTTACTAAATTCTGTGAAAGCATGTGGAGATTCACATTTTATTTATGTATAT
  TCTGCTATGGAATTAGATTTCTCTGGTCGTCACCTTGGTTCTGGGACATCCGACAGTGCT
  GGCATAACTATCCATTTCAGCCTCTTTCAAGTGGGCTNTATCACTATTATATCATGGAAT
  TGGCCTTCTATTGGTCCCTTATGTTTTCTCAGTTTACAGACATTAAAAGAAAAGACTTCC
  TGATCATGTTTGTGCATCACTTGGTCACCATTGGGCCTTATCTCCCTCTCCTACATCAAC
  CATATGGTTCCGAGTGGGGAACTCTGATCATGCGTCTACATGCAGGCTTAGACCTCCTGC
  CTGGAGCCAGCCAACTGGTCAATTATGCCCAGTATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_147190 unedited
  ACCTACTCACATCTGGCTGATGGCAACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTC
  ACAGGGATGCCACCCGGGATCTGTTCAGGAAACACTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTTGGGTTGAAGCACTTTTAATCACAAACAAACAATAGTA
  TAAGACCGTGAACAGCTCACCCAGTGGCTGTTGCCCTCCTCCAGCCCTCAGCTCACCCTC
  TTCCCATGCTCCCTCCCTGGGTACCATTGAGTAAAGAGCAGGGTATTGGCAGCTGTCCCT
  GTTGCTATCAGGTGATTGATAGGAAGGGGATCTAGCTAGGGACCACGGCAATCCTGGCCA
  TCCATCCATGGTTCTGTTTCTATACAACTTCATCATGTGACATATTCCAGCTGTGAAAGA
  GGAACAGTACAGAAATGTGGGACTGAACTTGGCTGCTTCATTCAGATGGTTCATGGGTTT
  AGAGTGTCAAGAGCCAAGGGCAAAAAAGAAGGAGAAGTCTGGAGTTGGCATAATTCAAGA
  TGCATCCCAATGTTGTACTAATGTGGGTTCAGTGGTGACCAGGGAAGGCCTCATGGTCCC
  ATTCCCACAATTGGGGCCCAACCCCGGCAATGAAACTTATGCTTATGCTCTATCGCCCCT
  CAGCAGATTCACTAAAACTTATAGTGGAAATAACATTACTGAAAGTACCTTGAGGTTTTT
  ATATACCAATGGCAGTTCTCCTCCAAAGTGAAAATTTTTGGTTTTTTTGATACTCCCTCA
  TGCGCCTGCAAGGCTTCATATCAGTTGGAGAGCTTTACTGGGCTAGGGCCAGCATCAGTA
  TTTTTCCACTCTGCCCCACCAAATCCAGCGGCAGCTGAAATCCCCTTAAACTACCGACTT
  TAATGTTCGTACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_147190
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147190.1, NP_671723.1
• RefSeq Size: 2022 bp
• RefSeq ORF: 1179 bp
• Locus ID: 91012
• UniProt ID: Q8N5B7
• Cytogenetics: 12q13.12
• Domains: homeobox, LAG1, TLC
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: This gene encodes a protein that belongs to the TLC (TRAM, LAG1 and CLN8 homology domains) family of proteins. The encoded protein functions in the synthesis of ceramide, a lipid molecule that is involved in a several cellular signaling pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.