Cathepsin L (CTSL) (NM_145918) Human Untagged Clone

CAT#: SC100267

CTSL1 (untagged)-Human cathepsin L1 (CTSL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin L
• Synonyms: CATL; CTSL1; MEP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145918, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCCTACACTCATCCTTGCTGCCTTTTGCCTGGGAATTGCCTCAGCTACTCTAACATTTGATCACA
  GTTTAGAGGCACAGTGGACCAAGTGGAAGGCGATGCACAACAGATTATACGGCATGAATGAAGAAGGATG
  GAGGAGAGCAGTGTGGGAGAAGAACATGAAGATGATTGAACTGCACAATCAGGAATACAGGGAAGGGAAA
  CACAGCTTCACAATGGCCATGAACGCCTTTGGAGACATGACCAGTGAAGAATTCAGGCAGGTGATGAATG
  GCTTTCAAAACCGTAAGCCCAGGAAGGGGAAAGTGTTCCAGGAACCTCTGTTTTATGAGGCCCCCAGATC
  TGTGGATTGGAGAGAGAAAGGCTACGTGACTCCTGTGAAGAATCAGGGTCAGTGTGGTTCTTGTTGGGCT
  TTTAGTGCTACTGGTGCTCTTGAAGGACAGATGTTCCGGAAAACTGGGAGGCTTATCTCACTGAGTGAGC
  AGAATCTGGTAGACTGCTCTGGGCCTCAAGGCAATGAAGGCTGCAATGGTGGCCTAATGGATTATGCTTT
  CCAGTATGTTCAGGATAATGGAGGCCTGGACTCTGAGGAATCCTATCCATATGAGGCAACAGAAGAATCC
  TGTAAGTACAATCCCAAGTATTCTGTTGCTAATGACACCGGCTTTGTGGACATCCCTAAGCAGGAGAAGG
  CCCTGATGAAGGCAGTTGCAACTGTGGGGCCCATTTCTGTTGCTATTGATGCAGGTCATGAGTCCTTCCT
  GTTCTATAAAGAAGGCATTTATTTTGAGCCAGACTGTAGCAGTGAAGACATGGATCATGGTGTGCTGGTG
  GTTGGCTACGGATTTGAAAGCACAGAATCAGATAACAATAAATATTGGCTGGTGAAGAACAGCTGGGGTG
  AAGAATGGGGCATGGGTGGCTACGTAAAGATGGCCAAAGACCGGAGAAACCATTGTGGAATTGCCTCAGC
  AGCCAGCTACCCCACTGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145918 unedited
  GTTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCCGCAAC
  CTTGAGCGGCATCCGTGGAGTGCGCCTGCGCAGCTACNACCGCAGCAGGAAAGCGCCGCC
  GGCCAGGCCCAGCTGTGGCCGGACAGGGACTGGAAGAGAGGACGCGGTCGAGTTTTAAAA
  CATGAATCCTACACTCATCCTTGCTGCCTTTTGCCTGGGAATTGCCTCAGCTACTCTAAC
  ATTTGATCACAGTTTAGAGGCACAGTGGACCAAGTGGAAGGCGATGCACAACAGATTATA
  CGGCATGAATGAAGAAGGATGGAGGAGAGCAGTGTGGGAGAAGAACATGAAGATGATTGA
  ACTGCACAATCAGGAATACAGGGAAGGGAAACACAGCTTCACAATGGCCATGAACGCCTT
  TGGAGACATGACCAGTGAAGAATTCAGGCAGGTGATGAATGGCTTTCAAAACCGTAAGCC
  CAGGAAGGGGAAAGTGTTCCAGGAACCTCTGTTTTATGAGGCCCCCAGATCTGTGGATTG
  GAGAGAGAAAGGCTACGTGACTCCTGTGAAGAATCAGGGTCAGTGTGGTTCTTGTTGGGC
  TTTTAGTGCTACTGGTGCTCTTGAAGGACAGATGTTCCGGANAACTGGGAGGCTTATCTC
  ACTGAGTGAGCAGAATCTGGTAGACTGCTCTGGGCCTCAAGGCAATGAAGGCTGCAATGG
  TGGCCTAATGGATTATGCTTTCCAGTATGTTCAGGATAATGGAGGCCTGGACTCTGAGGA
  ATCCTATCCATATGAGGGCACAGAAGAATCCCTGTAGTACATCCCCCAGTATTCTGTTGC
  TAATGACACCGGCTTTTGTGGACATNCCTTTAGCAGGAGAAGCCCTGATGAAGGCAGTGC
  ACCTGTGGGCCCCATTTCTGGTGTATGGATGCAGNCACTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145918 unedited
  GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTACATTTGAAAATTAAATTTTATTTAAACAGGGAAAAATTTATACATCC
  TTTTAAAAACGAAAATGCAAAGTCAGTAAGGGAATCAATAATATAAACCTATTTATAGCA
  GTAATTAAAATAGAGGGAACATTTACCAGGGGGAAAATATCACAGAATTCAAATCACACT
  CGGATCTTCAATGATTCGAGGGGGTATCCGACACAGGGGGGGCTGGTAAACTGAAAATGA
  ATTCCTCCCATGCATGCGCCATCCCCAGTCAAGTCCTTCCTTATCACCGTCCACCAGCTC
  ACACAGTGGGGTAACTGGCTGCTGAGGCAATTCCACAATGGTTTCTCCGGTCTTTGGCCA
  TTTTTACGTAGCCACCCATGCCCCATTCTTCACCCCAGCTGTTTTTCACCAGCCAATATT
  TATTGGTATCTGATTCTGGGCTTTCAAATCCGTAGCCAACCACCAGCACACCATGATCCA
  TGTCTTCACTGCTACAGTCTGGCTCAAAATAAATGCCTTCTTTATAGAACAGGAAGGACT
  CATGACCTGCATCAATAGCAACAGAAATGGGCCCCACAGTTGCAACTGCCTTCATCAGGG
  GCCTTCTCTGCTTAGGGATGTCCACAAAGCCGGTGTCATTAGCAACAGATACTTGGGATN
  GACCTACAGGATTCCCCTGTTGCCTCATATGGATAGGATTNCCTCAAAGTCAGGGCTNCA
  TTATCCTGAAACTACTGGAAAGCATATTCCTTTAGGCCACATTGCAGGCTTTAATTGCCT
  TGAGGGCCAAAAAAGTCTACAAATTTTGCNACCTCAGGAAAAAGGCTCCCAGTTTTTCGG
  AACATTGGCTTTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145918
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145918.1, NP_666023.1
• RefSeq Size: 1487 bp
• RefSeq ORF: 1002 bp
• Locus ID: 1514
• UniProt ID: P07711
• Cytogenetics: 9q21.33
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome
• Gene Summary: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1-4 encode the same isoform (1).