CHRDL1 (NM_145234) Human Untagged Clone

CAT#: SC100163

CHRDL1 (untagged)-Human chordin-like 1 (CHRDL1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRDL1
• Synonyms: CHL; dA141H5.1; MGC1; MGCN; NRLN1; VOPT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145234, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGAAAAAAGTGGAAAATGGGAGGCATGAAATACATCTTTTCGTTGTTGTTCTTTCTTTTGCTAGAAG
  GAGGCAAAACAGAGCAAGTAAAACATTCAGAGACATATTGCATGTTTCAAGACAAGAAGTACAGAGTGGG
  TGAGAGATGGCATCCTTACCTGGAACCTTATGGGTTGGTTTACTGCGTGAACTGCATCTGCTCAGAGAAT
  GGGAATGTGCTTTGCAGCCGAGTCAGATGTCCAAATGTTCATTGCCTTTCTCCTGTGCATATTCCTCATC
  TGTGCTGCCCTCGCTGCCCAGACTCCTTACCCCCAGTGAACAATAAGGTGACCAGCAAGTCTTGCGAGTA
  CAATGGGACAACTTACCAACATGGAGAGCTGTTCGTAGCTGAAGGGCTCTTTCAGAATCGGCAACCCAAT
  CAATGCACCCAGTGCAGCTGTTCGGAGGGAAACGTGTATTGTGGTCTCAAGACTTGCCCCAAATTAACCT
  GTGCCTTCCCAGTCTCTGTTCCAGATTCCTGCTGCCGGGTATGCAGAGGAGATGGAGAACTGTCATGGGA
  ACATTCTGATGGTGATATCTTCCGGCAACCTGCCAACAGAGAAGCAAGACATTCTTACCACCGCTCTCAC
  TATGATCCTCCACCAAGCCGACAGGCTGGAGGTCTGTCCCGCTTTCCTGGGGCCAGAAGTCACCGGGGAG
  CTCTTATGGATTCCCAGCAAGCATCAGGAACCATTGTGCAAATTGTCATCAATAACAAACACAAGCATGG
  ACAAGTGTGTGTTTCCAATGGAAAGACCTATTCTCATGGCGAGTCCTGGCACCCAAACCTCCGGGCATTT
  GGCATTGTGGAGTGTGTGCTATGTACTTGTAATGTCACCAAGCAAGAGTGTAAGAAAATCCACTGCCCCA
  ATCGATACCCCTGCAAGTATCCTCAAAAAATAGACGGAAAATGCTGCAAGGTGTGTCCAGGTAAAAAAGC
  AAAAGAACTTCCAGGCCAAAGCTTTGACAATAAAGGCTACTTCTGCGGGGAAGAAACGATGCCTGTGTAT
  GAGTCTGTATTCATGGAGGATGGGGAGACAACCAGAAAAATAGCACTGGAGACTGAGAGACCACCTCAGG
  TAGAGGTCCACGTTTGGACTATTCGAAAGGGCATTCTCCAGCACTTCCATATTGAGAAGATCTCCAAGAG
  GATGTTTGAGGAGCTTCCTCACTTCAAGCTGGTGACCAGAACAACCCTGAGCCAGTGGAAGATCTTCACC
  GAAGGAGAAGCTCAGATCAGCCAGATGTGTTCAAGTCGTGTATGCAGAACAGAGCTTGAAGATTTAGTCA
  AGGTTTTGTACCTGGAGAGATCTGAAAAGGGCCACTGTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145234 unedited
  GGTCAAATTTGTATACCATCACTATAGGCGGCCGCGATTCGGCACCAGGGAGCTCGGAGC
  GCGTGCACGCGTGGCAGACGGAGAAGGCCAGTGCCCAGCTTGAAGGTTCTGTCACCTTTT
  GCAGTGGTCCAAATGAGAAAAAAGTGGAAAATGGGAGGCATGAAATACATCTTTTCGTTG
  TTGTTCTTTCTTTTGCTAGAAGGAGGCAAAACAGAGCAAGTAAAACATTCAGAGACATAT
  TGCATGTTTCAAGACAAGAAGTACAGAGTGGGTGAGAGATGGCATCCTTACCTGGAACCT
  TATGGGTTGGTTTACTGCGTGAACTGCATCTGCTCAGAGAATGGGAATGTGCTTTGCAGC
  CGAGTCAGATGTCCAAATGTTCATTGCCTTTCTCCTGTGCATATTCCTCATCTGTGCTGC
  CCTCGCTGCCCAGAAGACTCCTTACCCCCAGTGAACAATAAGGTGACCAGCAAGTCTTGC
  GAGTACAATGGGACAACTTACCAACATGGAGAGCTGTTCGTAGCTGAAGGGCTCTTTCAG
  AATCGGCAACCCAATCAATGCACCCAGTGCAGCTGTTCGGAGGGAAACGTGTATTGTGGT
  CTCAAGACTTGCCCCAAATTAACCTGTGCCTTCCCAGTCTCTGTTCCAGATTCCTGCTGC
  CGGGTATGCAGAGGAGATGGAGAACTGTCATGGGAACATTCTGATGGTGATATCTTCCGG
  GCACCTGCCAACAGAGAAGCAAGACATTCTTACCACCGCTCTCACTATGATCCTNCACCA
  GCCGACAGGCTGGAGGTCTGTCCCGCTTTCCTGGGGCCAAAGTCACCGGNGAGCTCTTAT
  GGATTCCCAGCAGCATCNGAAACCATTGTGCAATGTCATCATAACAACACAGCTGGAACA
  GTGTGTGTTTCATGGNAAGACTATNTCATNGNCAGTCCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145234 unedited
  NNCCATTTGNNNGGGTATACTAGGACCGCGCCGCAATCTAAATACGCAATTTTTTTTTTT
  TTTTTTTTTTTGGAATTTCAAAATTAACTTTTTTATTAATTTAAAAATCCCGAAATACAG
  TGACTACCTAAATAAGTTCCATAATTAGGTACATGTCCTGTGAGAACAGTGAAAGGGTAA
  TACTGTTATGTTACTCTTACTTGTTTACATGAGTTAACTAGAAAATGGCTACCACTGCTA
  AATGATGCTTATGGTCTTTGTTGTTCCAAGTGTTTATGATACCATAAATCCCCAGAAGAA
  CCCATCCCTTCTTCTCTACTAACTACAGGCAGCTTGGCCTCTTTACCCTATGTCCTATTC
  TCTACACAACACCAAACACTGGAGGGTTTCTACTTTGACTTAACACAGCTCCCCAACTCC
  TGCTTCCCACAGCATTTTGCAAAGGTGTGTCCCAACACCTGGAAGCAAGAGTATATCTTG
  GAGAAACTCTCTGCGTGTTCTCTTTAGGCCAAACCTTTTCCAAAAACCCCTGGGTGGAGA
  ACGGCTTTGGGGGTGAATCCTTCCACAAAAGAGAAAACCACCTCTTTTGGCCTTAGGAAT
  CTAGCTTCCTAACCCCCCCCCTTTTAGGAAATCACCGACGCCCTCTCTGGGAACGGGAGG
  AGAGACCCCGCCAAAACAACATTACCGCCGTTTTAACGAAGAATTCCCCGAACAATGACA
  AGAGGAACCACCCTATTCACCCCCAGAACACCGGAACACGAGCGAAACTATAGACAGCCC
  CGCACCCCGGGAGAGGGACCCGAGAGGACACATCGTTCCATGANAACGACGGGCCTATAT
  AGAGCACACCAGAAAACAATATAACACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145234
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145234.2, NP_660277.2
• RefSeq Size: 2488 bp
• RefSeq ORF: 1371 bp
• Locus ID: 91851
• UniProt ID: Q9BU40
• Cytogenetics: Xq23
• Domains: VWC
• Protein Families: ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (3) differs in the 5' UTR and uses alternate in-frame splice sites in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.