Gabra1 (NM_183326) Rat Untagged Clone

CAT#: RN203113

Gabra1 (untagged ORF) - Rat gamma-aminobutyric acid (GABA) A receptor, alpha 1 (Gabra1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gabra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203113 representing NM_183326
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAAGTCGGGGTCTCTCTGACTATCTTTGGGCCTGGACCCTCATTCTGAGCACTCTCTCGGGAA
  GAAGCTATGGACAGCCCTCCCAAGATGAACTTAAGGACAACACCACTGTCTTCACGAGGATTTTGGACCG
  ACTGCTGGATGGTTATGACAATCGTCTGAGACCAGGCTTGGGAGAGCGTGTAACTGAAGTGAAGACGGAC
  ATCTTTGTCACCAGTTTCGGACCCGTGTCAGACCACGATATGGAATATACAATAGATGTGTTTTTCCGCC
  AAAGCTGGAAGGATGAAAGATTAAAATTCAAAGGACCCATGACAGTGCTCCGGCTGAACAACCTGATGGC
  CAGTAAAATCTGGACTCCAGATACATTTTTCCACAATGGAAAAAAGTCTGTGGCCCACAACATGACCATG
  CCCAATAAACTCCTGCGTATCACAGAGGATGGCACACTGCTGTACACCATGAGGTTGACTGTGAGAGCCG
  AATGCCCCATGCACTTAGAAGACTTTCCCATGGATGCCCATGCCTGCCCACTAAAATTTGGGAGCTATGC
  TTATACAAGAGCAGAAGTTGTCTATGAGTGGACAAGGGAGCCAGCCCGCTCAGTGGTTGTGGCAGAAGAT
  GGGTCACGTTTAAACCAGTATGACCTTCTTGGGCAAACAGTTGACTCTGGAATTGTTCAGTCCAGTACTG
  GAGAATATGTGGTTATGACGACTCACTTTCACTTGAAGAGAAAAATCGGCTACTTTGTTATTCAAACATA
  TCTGCCATGCATAATGACAGTCATTCTCTCCCAAGTCTCCTTCTGGCTTAACAGAGAGTCAGTACCAGCA
  AGAACTGTCTTTGGAGTGACGACCGTTCTGACCATGACAACCTTGAGTATCAGTGCCAGAAATTCCCTCC
  CAAAGGTGGCTTATGCAACGGCCATGGACTGGTTTATTGCAGTGTGCTATGCCTTCGTGTTCTCGGCTCT
  GATTGAGTTTGCCACAGTAAACTATTTCACCAAGAGAGGGTATGCGTGGGATGGCAAAAGCGTGGTTCCA
  GAAAAGCCAAAGAAAGTGAAGGATCCTCTCATTAAGAAAAACAACACATATGCTCCTACAGCAACCAGCT
  ATACCCCTAACTTAGCCAGGGGTGACCCCGGCTTGGCAACTATTGCTAAAAGTGCGACCATAGAACCGAA
  AGAAGTCAAGCCTGAGACAAAACCGCCAGAACCCAAGAAAACCTTTAACAGCGTCAGCAAAATCGACCGA
  CTGTCAAGAATAGCCTTTCCGCTGCTATTTGGAATCTTTAACTTAGTCTATTGGGCCACGTATTTAAACA
  GAGAGCCTCAGCTAAAAGCCCCCACACCCCATCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183326
• Insert Size: 1368 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183326.2, NP_899155.1
• RefSeq Size: 3637 bp
• RefSeq ORF: 1368 bp
• Locus ID: 29705
• UniProt ID: P62813
• Cytogenetics: 10q21
• Gene Summary: subunit of GABAA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]