DUS2L (DUS2) (NM_001271763) Human Tagged ORF Clone

CAT#: RG232782

DUS2L (tGFP-tagged) - Homo sapiens dihydrouridine synthase 2 (DUS2), transcript variant 3

Product Images

Circular map for RG232782

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUS2L
• Synonyms: DUS2L; SMM1; URLC8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232782 representing NM_001271763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTTCGGGTAGGGA
  CTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTACTGTGAGGAGCTGATCGACCT
  CAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTCAGCACAGTGGACTTTGTCGCCCCTGATGAT
  CGAGTTGTCTTCCGCACCTGTGAAAGAGAGCAGAACAGGGTGGTCTTCCAGATGGGAGGAATGGGAGCTG
  CCCTGCTGTCAGACCCTGACAAGATTGAGAAGATCCTCAGCACTCTTGTTAAAGGGACACGCAGACCTGT
  GACCTGCAAGATTCGCATCCTGCCATCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACT
  GGCATTGCTGCCATCGCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAG
  TCATCAAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCACATCCA
  ACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATGGTGGCCCGAGCAGCC
  ATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTGGAGGAGGTCATGCAGAAATACATCA
  GATACGCGGTGCAGTATGACAACCACTACACCAACACCAAGTACTGCTTGTGCCAGATGCTACGAGAACA
  GCTGGAGTCGCCCCAGGGAAGGTTGCTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGC
  CTTGGTGCCTTCTATGAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTT
  CAGAGCAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGACCGGAG
  AGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGGGAGAAGTTGGCACAG
  CCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCCTCTATTGTCACCGTTGCTGAACAAA
  AGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAACTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCG
  GAGCCAGGGCCTCCCTGAGGGTCGGCTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCT
  CCTGACCAAGACCCTGGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCT
  TTGTGGCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232782 representing NM_001271763
  Red=Cloning site Green=Tags(s)
  
  MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDD
  RVVFRTCEREQNRVVFQMGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERT
  GIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAA
  MWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFG
  LGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQ
  PVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREA
  PDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271763
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271763.2
• RefSeq Size: 2237 bp
• RefSeq ORF: 1377 bp
• Locus ID: 54920
• UniProt ID: Q9NX74
• Cytogenetics: 16q22.1
• Gene Summary: This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]