PBX3 (NM_001134778) Human Tagged ORF Clone

CAT#: RC225537

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PBX3 (Myc-DDK-tagged)-Human pre-B-cell leukemia homeobox 3 (PBX3), transcript variant 2



  "NM_001134778" in other vectors (4)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PBX3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225537 representing NM_001134778
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACCAGCGCTCTTCAGCGTCCTGTGTGAGATCAAAGAGAAAACAGGTCTCAGCATCAGAGGAGCCC
AGGAGGAGGACCCTCCCGATCCCCAGCTAATGAGACTGGACAATATGCTTTTGGCAGAAGGGGTTTCAGG
TCCTGAGAAAGGTGGGGGATCGGCGGCAGCAGCTGCAGCCGCGGCAGCCTCTGGAGGTTCTTCAGATAAC
TCTATTGAACACTCAGATTACAGAGCCAAATTGACCCAGATCAGACAAATCTATCACACAGAACTGGAGA
AATATGAACAGGCATGTAATGAATTTACTACACATGTGATGAACCTTCTCCGAGAACAGAGTAGAACACG
TCCCATTTCTCCAAAAGAGATTGAAAGAATGGTGGGCATCATCCATCGAAAATTTAGTTCCATTCAGATG
CAGCTCAAACAAAGCACTTGTGAAGCAGTTATGATTTTAAGATCAAGGTTCCTTGATGCCAGACGGAAAA
GGCGTAACTTCAGTAAACAGGCCACAGAAATCTTGAATGAATATTTTTACTCACACCTCAGCAACCCCTA
CCCCAGTGAAGAAGCCAAAGAGGAGCTGGCCAAGAAATGCAGCATCACAGTGTCACAGGTATCCAATTGG
TTTGGCAACAAACGAATCAGGTACAAGAAGAACATTGGCAAGTTTCAGGAAGAAGCCAACCTCTATGCTG
CAAAGACGGCCGTGACAGCTGCACACGCAGTAGCAGCAGCTGTGCAGAACAACCAGACCAATTCGCCCAC
CACACCAAATTCCGGTTCTTCTGGTTCTTTTAACCTCCCAAATTCTGGGGACATGTTCATGAACATGCAG
AGTCTGAATGGGGATTCTTACCAAGGGTCCCAAGTCGGAGCCAATGTGCAATCACAGGTGGATACCCTCC
GTCATGTTATCAATCAGACGGGAGGCTACAGTGATGGCCTTGGAGGAAATTCACTGTACAGTCCACATAA
TTTAAATGCTAATGGAGGCTGGCAGGACGCAACAACTCCATCTTCTGTGACTTCTCCTACAGAAGGCCCA
GGAAGTGTGCACTCGGATACCTCTAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225537 representing NM_001134778
Red=Cloning site Green=Tags(s)

MKPALFSVLCEIKEKTGLSIRGAQEEDPPDPQLMRLDNMLLAEGVSGPEKGGGSAAAAAAAAASGGSSDN
SIEHSDYRAKLTQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVGIIHRKFSSIQM
QLKQSTCEAVMILRSRFLDARRKRRNFSKQATEILNEYFYSHLSNPYPSEEAKEELAKKCSITVSQVSNW
FGNKRIRYKKNIGKFQEEANLYAAKTAVTAAHAVAAAVQNNQTNSPTTPNSGSSGSFNLPNSGDMFMNMQ
SLNGDSYQGSQVGANVQSQVDTLRHVINQTGGYSDGLGGNSLYSPHNLNANGGWQDATTPSSVTSPTEGP
GSVHSDTSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001134778
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001134778.2
RefSeq ORF 1080 bp
Locus ID 5090
UniProt ID P40426
Cytogenetics 9q33.3
Protein Families Transcription Factors
MW 39 kDa
Gene Summary Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'.[UniProtKB/Swiss-Prot Function]

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