NCOA1 (NM_003743) Human Tagged ORF Clone

CAT#: RC224812

NCOA1 (Myc-DDK-tagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1

Product Images

Circular map for RC224812

• 

  Western blot validation of overexpression lysate (Cat# LY401228) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224812 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCOA1
• Synonyms: bHLHe42; bHLHe74; F-SRC-1; KAT13A; RIP160; SRC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224812 representing NM_003743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCCTCGGGGACAGTTCATCCGACCCTGCTAACCCAGACTCACATAAGAGGAAAGGATCGCCAT
  GTGACACACTGGCATCAAGCACGGAAAAGAGGCGCAGGGAGCAAGAAAATAAATATTTAGAAGAACTAGC
  TGAGTTACTGTCTGCCAACATTAGTGACATTGACAGCTTGAGTGTAAAACCAGACAAATGCAAGATTTTG
  AAGAAAACAGTCGATCAGATACAGCTAATGAAGAGAATGGAACAAGAGAAATCAACAACTGATGACGATG
  TACAGAAATCAGACATCTCATCAAGTAGTCAAGGAGTGATAGAAAAGGAATCCTTGGGACCTCTTCTTTT
  GGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGGAGAATTGTATTTGTGTCAGAGAATGTA
  ACCAGCTACTTAGGTTACAATCAGGAGGAATTAATGAATACCAGCGTCTACAGCATACTGCACGTGGGGG
  ATCATGCAGAATTTGTGAAGAATCTGCTACCAAAATCACTAGTAAATGGAGTTCCTTGGCCTCAAGAGGC
  AACACGACGAAATAGCCATACCTTTAACTGCAGGATGCTAATTCACCCTCCAGATGAGCCAGGGACCGAG
  AACCAAGAAGCTTGCCAGCGTTATGAAGTAATGCAGTGTTTCACTGTGTCACAGCCAAAATCAATTCAAG
  AGGATGGAGAAGATTTCCAGTCATGTCTGATTTGTATTGCACGGCGATTACCTCGGCCTCCAGCTATTAC
  GGGTGTAGAATCCTTTATGACCAAGCAAGATACTACAGGTAAAATCATCTCTATTGATACTAGTTCCCTG
  AGAGCTGCTGGCAGAACTGGTTGGGAAGATTTAGTGAGGAAGTGCATTTATGCTTTTTTCCAACCTCAGG
  GCAGAGAACCATCTTATGCCAGACAGCTGTTCCAAGAAGTGATGACTCGTGGCACTGCCTCCAGCCCCTC
  CTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACCAAGTGTAAACTTTGCTACCCTCAA
  AGTCCAGACATGCAACCTTTCATCATGGGAATTCATATCATCGACAGGGAGCACAGTGGGCTTTCTCCTC
  AAGATGACACTAATTCTGGAATGTCAATTCCCCGAGTAAATCCCTCGGTCAATCCTAGTATCTCTCCAGC
  TCATGGTGTGGCTCGTTCATCCACATTGCCACCATCCAACAGCAACATGGTATCCACCAGAATAAACCGC
  CAGCAGAGCTCAGACCTTCATAGCAGCAGTCATAGTAATTCTAGCAACAGCCAAGGAAGTTTCGGATGCT
  CACCCGGAAGTCAGATTGTAGCCAATGTTGCCTTAAACCAAGGACAGGCCAGTTCACAGAGCAGTAATCC
  CTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATATCCCTAGCACAGTTCATGTCTCCAAGG
  AGACAGGTTACTTCTGGATTGGCAACAAGGCCCAGGATGCCAAACAATTCCTTTCCTCCTAATATTTCGA
  CATTAAGCTCTCCCGTTGGCATGACAAGTAGTGCCTGTAATAATAATAACCGATCTTATTCAAACATCCC
  AGTAACATCTTTACAGGGTATGAATGAAGGACCCAATAACTCCGTTGGCTTCTCTGCCAGTTCTCCAGTC
  CTCAGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAATATACAACCAGCAAAAGCTGAGTCCAAAG
  ATAACAAAGAGATTGCCTCAATTTTAAATGAAATGATTCAATCTGACAACAGCTCTAGTGATGGCAAACC
  TCTGGATTCAGGGCTTCTGCATAACAATGACAGACTTTCAGATGGAGACAGTAAATACTCTCAAACCAGT
  CACAAACTAGTGCAGCTTTTGACAACAACTGCCGAACAGCAGTTACGGCATGCTGATATAGACACAAGCT
  GCAAAGATGTCCTGTCTTGCACAGGCACTTCCAACTCTGCCTCTGCTAACTCTTCAGGAGGTTCTTGTCC
  CTCTTCTCATAGCTCATTGACAGAACGGCATAAAATTCTACACCGGCTCTTACAGGAGGGTAGCCCCTCA
  GATATCACCACTTTGTCTGTCGAGCCTGATAAAAAGGACAGTGCATCTACTTCTGTGTCAGTGACTGGAC
  AGGTACAAGGAAACTCCAGTATAAAACTAGAACTGGATGCTTCAAAGAAAAAAGAATCAAAAGACCATCA
  GCTCCTACGCTATCTTTTAGATAAAGATGAGAAAGATTTAAGATCAACTCCAAACCTGAGCCTGGATGAT
  GTAAAGGTGAAAGTGGAAAAGAAAGAACAGATGGATCCATGTAATACAAACCCAACCCCAATGACCAAAC
  CCACTCCTGAGGAAATAAAACTGGAGGCCCAGAGCCAGTTTACAGCTGACCTTGACCAGTTTGATCAGTT
  ACTGCCCACGCTGGAGAAGGCAGCACAGTTGCCAGGCTTATGTGAGACAGACAGGATGGATGGTGCGGTC
  ACCAGTGTAACCATCAAATCGGAGATCCTGCCAGCTTCACTTCAGTCCGCCACTGCCAGACCCACTTCCA
  GGCTAAATAGATTACCTGAGCTGGAATTGGAAGCAATTGATAACCAATTTGGACAACCAGGAACAGGCGA
  TCAGATTCCATGGACAAATAATACAGTGACAGCTATAAATCAGAGTAAATCAGAAGACCAGTGTATTAGC
  TCACAATTAGATGAGCTTCTCTGTCCACCCACAACAGTAGAAGGGAGAAATGATGAGAAGGCTCTTCTTG
  AACAGCTGGTATCCTTCCTTAGTGGCAAAGATGAAACTGAGCTAGCTGAACTAGACAGAGCTCTGGGAAT
  TGACAAACTTGTTCAGGGGGGTGGATTAGATGTATTATCAGAGAGATTTCCACCACAACAAGCAACGCCA
  CCTTTGATCATGGAAGAAAGACCCAACCTTTATTCCCAGCCTTACTCTTCTCCTTCTCCTACTGCCAATC
  TCCCTAGCCCTTTCCAAGGCATGGTCAGGCAAAAACCTTCACTGGGGACGATGCCTGTTCAAGTAACACC
  TCCCCGAGGTGCTTTTTCACCTGGCATGGGCATGCAGCCCAGGCAAACTCTAAACAGACCTCCGGCTGCA
  CCTAACCAGCTTCGACTTCAACTACAGCAGCGATTACAGGGACAACAGCAGTTGATACACCAAAATCGGC
  AAGCTATCTTAAACCAGTTTGCAGCAACTGCTCCTGTTGGCATCAATATGAGATCAGGCATGCAACAGCA
  AATTACACCTCAGCCACCCCTGAATGCTCAAATGTTGGCACAACGTCAGCGGGAACTGTACAGTCAACAG
  CACCGACAGAGGCAGCTAATACAGCAGCAAAGAGCCATGCTTATGAGGCAGCAAAGCTTTGGGAACAACC
  TCCCTCCCTCATCTGGACTACCAGTTCAAATGGGGAACCCCCGTCTTCCTCAGGGTGCTCCACAGCAATT
  CCCCTATCCACCAAACTATGGTACAAATCCAGGAACCCCACCTGCTTCTACCAGCCCGTTTTCACAACTA
  GCAGCAAATCCTGAAGCATCCTTGGCCAACCGCAACAGCATGGTGAGCAGAGGCATGACAGGAAACATAG
  GAGGACAGTTTGGCACTGGAATCAATCCTCAGATGCAGCAGAATGTCTTCCAGTATCCAGGAGCAGGAAT
  GGTTCCCCAAGGTGAGGCCAACTTTGCTCCATCTCTAAGCCCTGGGAGCTCCATGGTGCCGATGCCAATC
  CCTCCTCCTCAGAGTTCTCTGCTCCAGCAAACTCCACCTGCCTCCGGGTATCAGTCACCAGACATGAAGG
  CCTGGCAGCAAGGAGCGATAGGAAACAACAATGTGTTCAGTCAAGCTGTCCAGAACCAGCCCACGCCTGC
  ACAGCCAGGAGTATACAACAACATGAGCATCACCGTTTCCATGGCAGGTGGAAATACGAATGTTCAGAAC
  ATGAACCCAATGATGGCCCAGATGCAGATGAGCTCTTTGCAGATGCCAGGAATGAACACTGTGTGCCCTG
  AGCAGATAAATGATCCCGCACTGAGACACACAGGCCTCTACTGCAACCAGCTCTCATCCACTGACCTTCT
  CAAAACAGAAGCAGATGGAACCCAGCAGGTGCAACAGGTTCAGGTGTTTGCTGACGTCCAGTGTACAGTG
  AATCTGGTAGGCGGGGACCCTTACCTGAACCAGCCTGGTCCACTGGGAACTCAAAAGCCCACGTCAGGAC
  CACAGACCCCCCAGGCCCAGCAGAAGAGCCTCCTTCAGCAGCTACTGACTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224812 representing NM_003743
  Red=Cloning site Green=Tags(s)
  
  MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKIL
  KKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENV
  TSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTE
  NQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSL
  RAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQ
  SPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINR
  QQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPR
  RQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPV
  LRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTS
  HKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPS
  DITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDD
  VKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAV
  TSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCIS
  SQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATP
  PLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAA
  PNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQ
  HRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQL
  AANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPI
  PPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQN
  MNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTV
  NLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003743
• ORF Size: 4323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003743.5
• RefSeq Size: 6895 bp
• RefSeq ORF: 4326 bp
• Locus ID: 8648
• UniProt ID: Q15788
• Cytogenetics: 2p23.3
• Domains: PAS, HLH
• Protein Families: Druggable Genome, Transcription Factors
• MW: 156.6 kDa
• Gene Summary: The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]