hnRNP R (HNRNPR) (NM_005826) Human Tagged ORF Clone

CAT#: RC224502

HNRNPR (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein R (HNRNPR), transcript variant 2

Product Images

Circular map for RC224502

• 

  Western blot validation of overexpression lysate (Cat# LY417046) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224502 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HNRNPR protein (Cat# TP324502). The protein was produced from HEK293T cells transfected with HNRNPR cDNA clone (Cat# RC224502) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hnRNP R
• Synonyms: hnRNP-R; HNRPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224502 representing NM_005826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAATCAGGTGAATGGTAATGCGGTACAGTTAAAAGAAGAGGAAGAACCAATGGATACTTCCAGTG
  TAACTCACACAGAACACTACAAGACACTGATAGAGGCAGGCCTCCCACAGAAGGTGGCAGAAAGACTTGA
  TGAAATATTTCAGACAGGATTGGTAGCTTATGTCGATCTTGATGAAAGAGCAATTGATGCTCTCAGGGAA
  TTTAATGAAGAAGGAGCTCTGTCTGTACTACAGCAGTTCAAGGAAAGTGACTTATCACATGTTCAGAACA
  AAAGTGCATTTTTATGTGGAGTTATGAAGACCTACAGGCAGAGAGAGAAACAGGGGAGCAAGGTGCAAGA
  GTCCACAAAGGGACCTGATGAAGCGAAGATCAAGGCCTTGCTTGAGAGAACTGGTTATACTCTGGATGTA
  ACCACAGGACAGAGGAAGTATGGTGGTCCTCCACCAGACAGTGTGTACTCTGGCGTGCAACCTGGAATTG
  GAACGGAGGTATTTGTAGGCAAAATACCAAGGGATTTATATGAGGATGAGTTGGTGCCCCTTTTTGAGAA
  GGCCGGACCCATTTGGGATCTACGTCTTATGATGGATCCACTGTCCGGTCAGAATAGAGGGTATGCATTT
  ATCACCTTCTGTGGAAAGGAAGCTGCACAGGAAGCCGTGAAACTGTGTGACAGCTATGAAATTCGCCCTG
  GTAAACACCTTGGAGTGTGCATTTCTGTGGCAAACAACAGACTTTTTGTTGGATCCATTCCGAAGAATAA
  GACTAAAGAAAACATTTTGGAAGAATTCAGTAAAGTCACAGAGGGTTTGGTGGACGTTATTCTCTATCAT
  CAACCCGATGACAAAAAGAAGAATCGGGGGTTCTGCTTCCTTGAATATGAGGATCACAAGTCAGCAGCAC
  AAGCCAGACGCCGGCTGATGAGTGGAAAAGTAAAAGTGTGGGGAAATGTAGTTACAGTTGAATGGGCTGA
  CCCTGTGGAAGAACCAGATCCAGAAGTCATGGCTAAGGTAAAAGTTTTGTTTGTGAGAAACTTGGCTACT
  ACGGTGACAGAAGAAATATTGGAAAAGTCATTTTCTGAATTTGGAAAACTCGAAAGAGTAAAGAAGTTGA
  AAGATTATGCATTTGTTCATTTTGAAGACAGAGGAGCAGCTGTTAAGGCTATGGATGAAATGAATGGCAA
  AGAAATAGAAGGGGAAGAAATTGAAATAGTCTTAGCCAAGCCACCAGACAAGAAAAGGAAAGAGCGCCAA
  GCTGCTAGACAGGCCTCCAGAAGCACTGCGTATGAAGATTATTACTACCACCCTCCTCCTCGCATGCCAC
  CTCCAATTAGAGGTCGGGGTCGTGGTGGGGGGAGAGGTGGATATGGCTACCCTCCAGATTACTACGGCTA
  TGAAGATTACTATGATGATTACTATGGTTATGATTATCACGACTATCGTGGAGGCTATGAAGATCCCTAC
  TACGGCTATGATGATGGCTATGCAGTAAGAGGAAGAGGAGGAGGAAGGGGAGGGCGAGGTGCTCCACCAC
  CACCAAGGGGGAGGGGAGCACCACCTCCAAGAGGTAGAGCTGGCTATTCACAGAGGGGGGCACCTTTGGG
  ACCACCAAGAGGCTCTAGGGGTGGCAGAGGGGGTCCTGCTCAACAGCAGAGAGGCCGTGGTTCCCGTGGA
  TCTCGGGGCAATCGTGGGGGCAATGTAGGAGGCAAGAGAAAGGCAGATGGGTACAACCAGCCTGATTCCA
  AGCGTCGTCAGACCAACAACCAACAGAACTGGGGTTCCCAACCCATCGCTCAGCAGCCGCTTCAGCAAGG
  TGGTGACTATTCTGGTAACTATGGTTACAATAATGACAACCAGGAATTTTATCAGGATACTTATGGGCAA
  CAGTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224502 representing NM_005826
  Red=Cloning site Green=Tags(s)
  
  MANQVNGNAVQLKEEEEPMDTSSVTHTEHYKTLIEAGLPQKVAERLDEIFQTGLVAYVDLDERAIDALRE
  FNEEGALSVLQQFKESDLSHVQNKSAFLCGVMKTYRQREKQGSKVQESTKGPDEAKIKALLERTGYTLDV
  TTGQRKYGGPPPDSVYSGVQPGIGTEVFVGKIPRDLYEDELVPLFEKAGPIWDLRLMMDPLSGQNRGYAF
  ITFCGKEAAQEAVKLCDSYEIRPGKHLGVCISVANNRLFVGSIPKNKTKENILEEFSKVTEGLVDVILYH
  QPDDKKKNRGFCFLEYEDHKSAAQARRRLMSGKVKVWGNVVTVEWADPVEEPDPEVMAKVKVLFVRNLAT
  TVTEEILEKSFSEFGKLERVKKLKDYAFVHFEDRGAAVKAMDEMNGKEIEGEEIEIVLAKPPDKKRKERQ
  AARQASRSTAYEDYYYHPPPRMPPPIRGRGRGGGRGGYGYPPDYYGYEDYYDDYYGYDYHDYRGGYEDPY
  YGYDDGYAVRGRGGGRGGRGAPPPPRGRGAPPPRGRAGYSQRGAPLGPPRGSRGGRGGPAQQQRGRGSRG
  SRGNRGGNVGGKRKADGYNQPDSKRRQTNNQQNWGSQPIAQQPLQQGGDYSGNYGYNNDNQEFYQDTYGQ
  QWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005826
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005826.5
• RefSeq Size: 2663 bp
• RefSeq ORF: 1902 bp
• Locus ID: 10236
• UniProt ID: O43390
• Cytogenetics: 1p36.12
• Domains: RRM
• MW: 70.8 kDa
• Gene Summary: This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10. [provided by RefSeq, Jul 2014]