SP3 (NM_003111) Human Tagged ORF Clone

CAT#: RC222027

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SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 1



Reconstitution Protocol

USD 715.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SP3
Synonyms SPR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222027 representing NM_003111
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGCTCCCGAAAAGCCCGTGAAACAAGAGGAAATGGCTGCCTTGGACGTGGATAGCGGCGGCGGCG
GTGGCGGCGGCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCACGGAAACGGTGCGGTGGCGGC
GGCAGCGGCGGCCCAGGACACTCAGCCGTCACCGCTCGCTCTGCTGGCCGCTACCTGCAGCAAGATAGGG
CCGCCATCGCCGGGCGACGACGAGGAGGAGGCGGCCGCCGCAGCCGGGGCCCCCGCCGCCGCCGGAGCGA
CAGGTGATTTGGCTTCTGCACAGTTAGGAGGAGCACCAAACCGATGGGAGGTTTTGTCAGCCACACCTAC
AACTATAAAAGATGAAGCTGGTAATCTAGTCCAGATTCCAAGTGCTGCTACTTCAAGTGGGCAGTATGTT
CTTCCCCTTCAGAATTTGCAGAATCAACAAATATTTTCCGTTGCACCAGGATCAGATTCATCAAATGGTG
CAGTGTCCAGTGTTCAATATCAAGTGATACCACAGATCCAGTCAGCAGATGGTCAGCAGGTTCAAATTGG
TTTCACAGGCTCTTCAGATAATGGGGGTATAAATCAAGAAAGCAGTCAAATTCAGATCATTCCTGGCTCT
AATCAAACCTTACTTGCCTCTGGAACACCTTCTGCTAACATCCAGAATCTCATACCACAGACTGGTCAAG
TCCAGGTTCAGGGAGTTGCAATTGGTGGTTCATCTTTTCCTGGTCAAACCCAAGTAGTTGCTAATGTGCC
TCTTGGTCTGCCAGGAAATATTACGTTTGTACCAATCAATAGTGTCGATCTAGATTCTTTGGGACTCTCG
GGCAGTTCTCAGACAATGACTGCAGGCATTAATGCCGACGGACATTTGATAAACACAGGACAAGCTATGG
ATAGTTCAGACAATTCAGAAAGGACTGGTGAGCGGGTTTCTCCTGATATTAATGAAACTAATACTGATAC
AGATTTATTTGTGCCAACATCCTCTTCATCACAGTTGCCTGTTACGATAGATAGTACAGGTATATTACAA
CAAAACACAAATAGCTTGACTACATCTAGTGGGCAGGTTCATTCTTCAGATCTTCAGGGAAATTATATCC
AGTCGCCTGTTTCTGAAGAGACACAGGCACAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAGCA
TCTACAACTTCAAGAGTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACA
ATCCATGGTGTGCAAGCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAATCTTCAGTTGCAGCTGA
ATCCTGGAACCTTTTTAATTCAGGCACAGACAGTGACCCCTTCTGGACAGGTAACTTGGCAAACGTTTCA
AGTACAAGGGGTCCAGAACTTGCAGAATTTGCAAATACAGAATACTGCTGCCCAACAAATAACTTTGACG
CCTGTTCAAACCCTCACACTTGGTCAAGTTGCGGCAGGTGGAGCCTTCACTTCAACTCCAGTTAGTCTAA
GCACTGGTCAGTTGCCAAATCTACAAACAGTTACAGTGAACTCTATAGATTCTGCTGGTATACAGCTACA
TCCAGGAGAGAATGCTGACAGTCCTGCAGATATTAGGATCAAGGAAGAAGAACCTGATCCTGAAGAGTGG
CAGCTCAGTGGTGATTCTACCTTGAATACCAATGACCTAACACACTTAAGAGTACAGGTGGTAGATGAAG
AAGGGGACCAACAACATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGCACCTGTCCCAACTGTAAAGA
AGGTGGTGGAAGAGGTACCAATCTTGGGAAAAAGAAGCAACACATTTGTCATATACCAGGATGTGGTAAA
GTCTATGGGAAGACCTCACATCTGAGAGCTCATCTGCGTTGGCATTCTGGAGAACGCCCTTTTGTTTGTA
ACTGGATGTACTGTGGTAAAAGATTTACTCGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGG
TGAGAAGAAATTTGTTTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATT
AAAACACACCAGAATAAAAAAGGTATTCACTCTAGCAGTACAGTGCTGGCATCTGTGGAAGCTGCGCGAG
ATGATACTTTGATTACTGCAGGAGGAACAACGCTTATCCTTGCAAATATTCAACAAGGTTCTGTTTCAGG
GATAGGAACTGTTAATACTTCCGCCACCAGCAATCAAGATATCCTTACCAACACTGAAATACCTTTACAG
CTTGTCACAGTTTCTGGAAATGAGACAATGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222027 representing NM_003111
Red=Cloning site Green=Tags(s)

MTAPEKPVKQEEMAALDVDSGGGGGGGGGHGEYLQQQQQHGNGAVAAAAAAQDTQPSPLALLAATCSKIG
PPSPGDDEEEAAAAAGAPAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPSAATSSGQYV
LPLQNLQNQQIFSVAPGSDSSNGAVSSVQYQVIPQIQSADGQQVQIGFTGSSDNGGINQESSQIQIIPGS
NQTLLASGTPSANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLS
GSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQ
QNTNSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQT
IHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQVTWQTFQVQGVQNLQNLQIQNTAAQQITLT
PVQTLTLGQVAAGGAFTSTPVSLSTGQLPNLQTVTVNSIDSAGIQLHPGENADSPADIRIKEEEPDPEEW
QLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGK
VYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHI
KTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQ
LVTVSGNETME

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003111
ORF Size 2343 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003111.4
RefSeq Size 3920 bp
RefSeq ORF 2346 bp
Locus ID 6670
UniProt ID Q02447
Cytogenetics 2q31.1
Protein Families Druggable Genome, Transcription Factors
MW 81.7 kDa
Gene Summary This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
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