C14orf130 (UBR7) (NM_175748) Human Tagged ORF Clone

CAT#: RC218298

UBR7 (Myc-DDK-tagged)-Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 2

Product Images

Circular map for RC218298

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  Western blot validation of overexpression lysate (Cat# LY403580) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218298 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBR7 protein (Cat# TP318298). The protein was produced from HEK293T cells transfected with UBR7 cDNA clone (Cat# RC218298) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C14orf130
• Synonyms: C14orf130; LICAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218298 representing NM_175748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGCCGAGGGCGCCGCTGGGCGGCAGTCGGAGCTGGAGCCCGTGGTATCGTTGGTCGACGTCC
  TTGAGGAGGACGAGGAGCTGGAGAATGAGGCGTGCGCTGTCCTGGGCGGCAGCGACTCCGAGAAGTGCTC
  CTACTCTCAGGGCTCAGTAAAGAGACAAGCACTATATGCCTGTAGTACCTGCACCCCAGAGGGAGAAGAA
  CCAGCAGGAATTTGTTTAGCTTGCAGTTATGAATGTCATGGAAGTCACAAACTATTTGAGCTATACACAA
  AAAGAAATTTTCGTTGTGATTGTGGAAACAGCAAGTTTAAAAATTTGGAATGCAAATTACTTCCTGACAA
  AGCAAAGGTAAATTCTGGCAATAAGTACAATGACAACTTTTTTGGATTGTACTGCATTTGCAAGAGACCT
  TATCCTGATCCTGAAGACGAGATTCCAGATGAGATGATCCAGTGCGTAGTCTGTGAAGACTGGTTCCATG
  GAAGGCATCTTGGTGCCATTCCCCCTGAGAGTGGGGATTTTCAGGAGATGGTATGCCAGGCCTGCATGAA
  ACGTTGTTCTTTTTTGTGGGCTTATGCTGCACAATTGGCAGTAACCAAAATATCCACTGAGGATGATGGA
  TTGGTGCGGAACATTGATGGAATAGGTGATCAGGAAGTTATCAAACCTGAAAATGGAGAGCATCAAGATA
  GTACCCTCAAAGAGGATGTTCCAGAACAGGGAAAGGATGATGTCCGGGAGGTTAAAGTAGAGCAGAACAG
  TGAACCATGTGCCGGCTCTAGTTCTGAATCTGATCTCCAGACAGTGTTTAAGAATGAAAGCCTCAACGCA
  GAATCAAAATCTGGCTGCAAACTTCAGGAGCTTAAAGCTAAGCAGCTTATAAAGAAAGACACTGCCACCT
  ATTGGCCCCTGAACTGGCGTAGCAAGTTGTGTACCTGCCAAGACTGTATGAAAATGTATGGAGATCTAGA
  TGTCTTATTCCTGACAGATGAATACGACACAGTTCTGGCTTATGAAAACAAAGGGAAGATTGCCCAGGCC
  ACTGACAGGAGCGATCCCCTAATGGATACCCTTAGCAGCATGAATAGAGTCCAGCAAGTGGAACTCATTT
  GTGAATACAATGATTTGAAGACTGAACTTAAAGACTATCTCAAGAGATTTGCTGATGAAGGCACGGTTGT
  TAAGAGAGAGGACATTCAGCAGTTCTTTGAAGAGTTTCAGTCAAAAAAGAGAAGAAGAGTGGATGGGATG
  CAGTATTACTGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218298 representing NM_175748
  Red=Cloning site Green=Tags(s)
  
  MAGAEGAAGRQSELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEE
  PAGICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFGLYCICKRP
  YPDPEDEIPDEMIQCVVCEDWFHGRHLGAIPPESGDFQEMVCQACMKRCSFLWAYAAQLAVTKISTEDDG
  LVRNIDGIGDQEVIKPENGEHQDSTLKEDVPEQGKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLNA
  ESKSGCKLQELKAKQLIKKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGKIAQA
  TDRSDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGM
  QYYCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175748
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175748.4
• RefSeq Size: 1576 bp
• RefSeq ORF: 1278 bp
• Locus ID: 55148
• UniProt ID: Q8N806
• Cytogenetics: 14q32.12
• Protein Families: Druggable Genome
• MW: 47.8 kDa
• Gene Summary: This gene encodes a UBR box-containing protein that belongs to the E3 ubiquitin ligase family. The protein also contains a plant homeodomain (PHD) in the C-terminus. In mammals, the encoded protein recognizes N-degrons, the destabilizing N-terminal residues of short-lived proteins, which results in ubiquitinylation, and proteolysis via the proteasome. [provided by RefSeq, Jul 2016]