C14orf130 (UBR7) (NM_175748) Human Tagged ORF Clone

CAT#: RC218298

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UBR7 (Myc-DDK-tagged)-Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 2



  "NM_175748" in other vectors (6)

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USD 477.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol UBR7
Synonyms C14orf130; LICAS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218298 representing NM_175748
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGAGCCGAGGGCGCCGCTGGGCGGCAGTCGGAGCTGGAGCCCGTGGTATCGTTGGTCGACGTCC
TTGAGGAGGACGAGGAGCTGGAGAATGAGGCGTGCGCTGTCCTGGGCGGCAGCGACTCCGAGAAGTGCTC
CTACTCTCAGGGCTCAGTAAAGAGACAAGCACTATATGCCTGTAGTACCTGCACCCCAGAGGGAGAAGAA
CCAGCAGGAATTTGTTTAGCTTGCAGTTATGAATGTCATGGAAGTCACAAACTATTTGAGCTATACACAA
AAAGAAATTTTCGTTGTGATTGTGGAAACAGCAAGTTTAAAAATTTGGAATGCAAATTACTTCCTGACAA
AGCAAAGGTAAATTCTGGCAATAAGTACAATGACAACTTTTTTGGATTGTACTGCATTTGCAAGAGACCT
TATCCTGATCCTGAAGACGAGATTCCAGATGAGATGATCCAGTGCGTAGTCTGTGAAGACTGGTTCCATG
GAAGGCATCTTGGTGCCATTCCCCCTGAGAGTGGGGATTTTCAGGAGATGGTATGCCAGGCCTGCATGAA
ACGTTGTTCTTTTTTGTGGGCTTATGCTGCACAATTGGCAGTAACCAAAATATCCACTGAGGATGATGGA
TTGGTGCGGAACATTGATGGAATAGGTGATCAGGAAGTTATCAAACCTGAAAATGGAGAGCATCAAGATA
GTACCCTCAAAGAGGATGTTCCAGAACAGGGAAAGGATGATGTCCGGGAGGTTAAAGTAGAGCAGAACAG
TGAACCATGTGCCGGCTCTAGTTCTGAATCTGATCTCCAGACAGTGTTTAAGAATGAAAGCCTCAACGCA
GAATCAAAATCTGGCTGCAAACTTCAGGAGCTTAAAGCTAAGCAGCTTATAAAGAAAGACACTGCCACCT
ATTGGCCCCTGAACTGGCGTAGCAAGTTGTGTACCTGCCAAGACTGTATGAAAATGTATGGAGATCTAGA
TGTCTTATTCCTGACAGATGAATACGACACAGTTCTGGCTTATGAAAACAAAGGGAAGATTGCCCAGGCC
ACTGACAGGAGCGATCCCCTAATGGATACCCTTAGCAGCATGAATAGAGTCCAGCAAGTGGAACTCATTT
GTGAATACAATGATTTGAAGACTGAACTTAAAGACTATCTCAAGAGATTTGCTGATGAAGGCACGGTTGT
TAAGAGAGAGGACATTCAGCAGTTCTTTGAAGAGTTTCAGTCAAAAAAGAGAAGAAGAGTGGATGGGATG
CAGTATTACTGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218298 representing NM_175748
Red=Cloning site Green=Tags(s)

MAGAEGAAGRQSELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEE
PAGICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFGLYCICKRP
YPDPEDEIPDEMIQCVVCEDWFHGRHLGAIPPESGDFQEMVCQACMKRCSFLWAYAAQLAVTKISTEDDG
LVRNIDGIGDQEVIKPENGEHQDSTLKEDVPEQGKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLNA
ESKSGCKLQELKAKQLIKKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGKIAQA
TDRSDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGM
QYYCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_175748
ORF Size 1275 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175748.4
RefSeq Size 1576 bp
RefSeq ORF 1278 bp
Locus ID 55148
UniProt ID Q8N806
Cytogenetics 14q32.12
Protein Families Druggable Genome
MW 47.8 kDa
Gene Summary This gene encodes a UBR box-containing protein that belongs to the E3 ubiquitin ligase family. The protein also contains a plant homeodomain (PHD) in the C-terminus. In mammals, the encoded protein recognizes N-degrons, the destabilizing N-terminal residues of short-lived proteins, which results in ubiquitinylation, and proteolysis via the proteasome. [provided by RefSeq, Jul 2016]

Other Versions

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