L2HGDH (NM_024884) Human Tagged ORF Clone

CAT#: RC217631

L2HGDH (Myc-DDK-tagged)-Human L-2-hydroxyglutarate dehydrogenase (L2HGDH), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY410989) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217631 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified L2HGDH protein (Cat# TP317631). The protein was produced from HEK293T cells transfected with L2HGDH cDNA clone (Cat# RC217631) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2HGDH
• Synonyms: C14orf160; L2HGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217631 representing NM_024884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCAGCGCTGCGTTATTTGGTTGGTGCCTGCGGACGGGCCCGCGGGCGTTTCGCCGGTGGCTCCC
  CTGGGGCGTGCGGGTTCGCGTCTGGGAGGCCAAGACCGCTGTGTGGAGGTAGCCGCAGCGCCAGCACCAG
  CTCATTTGATATAGTCATTGTTGGTGGCGGAATTGTGGGGCTTGCCTCTGCCAGAGCACTCATCCTGCGA
  CATCCATCACTTTCTATTGGTGTTCTGGAAAAGGAGAAAGATTTAGCTGTTCACCAGACTGGACATAACA
  GTGGTGTCATACATAGTGGAATTTATTATAAACCTGAGTCTCTGAAAGCCAAATTATGTGTACAAGGTGC
  AGCCCTCCTCTATGAGTACTGTCAGCAAAAGGGAATTTCCTACAAGCAGTGTGGCAAGCTTATAGTAGCT
  GTTGAACAAGAAGAAATTCCCAGACTTCAGGCCCTATATGAGAAAGGCCTCCAGAATGGTGTCCCGGGCC
  TGAGGCTGATCCAGCAGGAGGATATAAAAAAGAAGGAGCCATATTGTAGGGGTCTAATGGCTATTGATTG
  TCCACATACTGGCATTGTGGACTATCGGCAGGTGGCTTTGTCATTTGCCCAGGATTTCCAAGAAGCAGGT
  GGCTCTGTCTTGACCAATTTTGAAGTAAAAGGTATTGAAATGGCTAAAGAAAGTCCTTCAAGAAGTATAG
  ATGGAATGCAATATCCAATTGTTATAAAGAATACAAAGGGAGAGGAAATTCGATGTCAGTATGTTGTGAC
  ATGTGCAGGACTTTACTCAGACCGTATTTCAGAGTTGAGTGGCTGCACTCCTGATCCTCGAATTGTACCA
  TTCCGGGGAGATTACCTGCTTTTGAAGCCAGAAAAATGTTATCTTGTAAAAGGAAATATTTATCCGGTCC
  CAGATAGCCGGTTTCCTTTCCTAGGAGTTCACTTCACACCAAGGATGGATGGCAGTATTTGGCTAGGGCC
  TAATGCAGTTCTTGCCTTTAAACGAGAGGGTTACAGACCCTTTGACTTCAGTGCCACAGATGTTATGGAT
  ATAATTATCAATAGTGGCTTGATTAAACTGGCATCCCAGAATTTTTCCTATGGAGTTACTGAAATGTATA
  AAGCATGTTTTCTTGGTGCAACAGTGAAGTATCTTCAAAAATTCATCCCTGAAATTACTATCAGTGATAT
  ACTTAGGGGCCCAGCTGGAGTAAGAGCCCAGGCCCTGGATAGAGATGGAAATCTGGTAGAAGATTTTGTA
  TTTGATGCAGGAGTTGGGGATATTGGAAATCGCATTCTTCATGTGAGAAATGCACCTTCTCCTGCTGCTA
  CTTCTTCCATTGCAATTTCTGGAATGATTGCAGATGAAGTACAACAAAGATTTGAATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217631 representing NM_024884
  Red=Cloning site Green=Tags(s)
  
  MVPALRYLVGACGRARGRFAGGSPGACGFASGRPRPLCGGSRSASTSSFDIVIVGGGIVGLASARALILR
  HPSLSIGVLEKEKDLAVHQTGHNSGVIHSGIYYKPESLKAKLCVQGAALLYEYCQQKGISYKQCGKLIVA
  VEQEEIPRLQALYEKGLQNGVPGLRLIQQEDIKKKEPYCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAG
  GSVLTNFEVKGIEMAKESPSRSIDGMQYPIVIKNTKGEEIRCQYVVTCAGLYSDRISELSGCTPDPRIVP
  FRGDYLLLKPEKCYLVKGNIYPVPDSRFPFLGVHFTPRMDGSIWLGPNAVLAFKREGYRPFDFSATDVMD
  IIINSGLIKLASQNFSYGVTEMYKACFLGATVKYLQKFIPEITISDILRGPAGVRAQALDRDGNLVEDFV
  FDAGVGDIGNRILHVRNAPSPAATSSIAISGMIADEVQQRFEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024884
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024884.3
• RefSeq Size: 2064 bp
• RefSeq ORF: 1392 bp
• Locus ID: 79944
• UniProt ID: Q9H9P8
• Cytogenetics: 14q21.3
• Protein Pathways: Butanoate metabolism
• MW: 45.2 kDa
• Gene Summary: This gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive neurometabolic disorder resulting in moderate to severe cognitive disability. [provided by RefSeq, Jul 2008]